2A protein (NC_001428) Virus Tagged ORF Clone

CAT#: VC100744

Myc-DDK-tagged ORF clone of viral ORF for 2A protein [Human enterovirus C], codon optimized for human cell expression, NP_740587

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 2A protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100744 represents NCBI reference of NP_740587 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTTTGGTCACCAAAATAAAGCCGTATATGTCGCTGGGTATAAAATCTGCAATTACCACCTTGCTA
  CACCCTCTGACCACCTCAATGCTATATCCGTGCTCTGGGATCGCGATCTGATGGTCGTTGAGTCAAGGGC
  ACAAGGCACTGATACCATAGCCAGGTGCAGCTGCAGGTGCGGAGTCTACTATTGCGAGTCACGGAGGAAA
  TACTATCTGGTGACTTTCACCGGACCAACATTTAGGTTTATGGAGGCCAACGATTATTACCCCGCACGCT
  ACCAATCCCATATGTTGATTGGGTGTGGGTTCGCGGAACCTGGTGATTGCGGGGGGATTCTGAGGTGCAC
  ACACGGTGTGATCGGCATTATCACAGCTGGGGGCGAAGGTATCGTGGCCTTTGCTGATATCAGGGATTTG
  TGGGTATACGAAGAGGAGGCGATGGAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100744 representing NP_740587
  Red=Cloning sites Green=Tags
  
  MGFGHQNKAVYVAGYKICNYHLATPSDHLNAISVLWDRDLMVVESRAQGTDTIARCSCRCGVYYCESRRK
  YYLVTFTGPTFRFMEANDYYPARYQSHMLIGCGFAEPGDCGGILRCTHGVIGIITAGGEGIVAFADIRDL
  WVYEEEAMEQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001428
• ORF Size: 450 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001428.1, NP_740587
• RefSeq ORF: 450
• MW: 16.8 kDa