2C protein (NC_001428) Virus Tagged ORF Clone

CAT#: VC100746

Myc-DDK-tagged ORF clone of viral ORF for 2C protein [Human enterovirus C], codon optimized for human cell expression, NP_740589

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 2C protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100746 represents NCBI reference of NP_740589 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGACGGGTGGATGAAGAAATTCACAGAGGCCTGCAATGCTGCCAAGGGCCTGAGATGGGTGTCCA
  ATAAAATCTCCAAATTTGTGGACTGGCTGAAGTGTAAAATAATCCCAGAAGCGAAGGACAAAGTGGAATT
  CCTTACAAAGCTCAAGCAACTGGATATGCTGGAGAATCAGATCGCGACTATCCACCAGAGTTGCCCATCA
  CAGGAACAACAAGAAATTCTCTTCAACAACGTGCGGTGGCTGGCTGTTCAGAGCCGGAGGTTTGCTCCTC
  TTTATGCTGTTGAAGCCAGACGGATAAGCAAGATGGAGAGCACCATCAATAATTATATTCAGTTCAAATC
  CAAACATCGCATTGAGCCGGTATGCATGTTGGTACACGGCTCACCTGGGACTGGGAAAGGTATAGCCAGC
  TCCCTGATTGGGAGGGCCATCGCTGAGCGCGAAACCACCTCCGTCTACTCCGTACCTCTCGCCCCCTCTC
  ACTTCGATGGCTATAAGCAACAAGGTTACGACATGGACGACTTGAACCAGAACCCTGATGGTATGGATAT
  GAAGCTGTTCTGCCAGATGGTAAGCACCGTCGAGTTTATCCCTCCCATGGCATCACTCGAAGAAAAAGGC
  ATATTGTTTACAAGCGACTATGTGCTCGCCTCCACAAATAGCCATTCAATCGCGCCTCCCACCGTCGCCC
  ACTCCGATGCACTCACAAGGCGGTTCGCGTTCGACGTAGAGGTTTACACCATGTCTGAACATAGTGTAAA
  AGGTAAGCTCAACATGGCGACCGCCACACAGCTCTGTAAGGACTGTCCTACTCCAGCTAATTTCAAAAAG
  TGCTGCCCCCTTGTGTGCGGCAAGGCACTGCAGCTGATGGATCGCTACACAAGACAGCGGTTTACCGTGG
  ATGAAATTACAACACTGATCATGAACGAGAAGAATCGGCGAGCAAATATTGGAAACTGTATGGAAGCTTT
  GTTCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100746 representing NP_740589
  Red=Cloning sites Green=Tags
  
  MGDGWMKKFTEACNAAKGLRWVSNKISKFVDWLKCKIIPEAKDKVEFLTKLKQLDMLENQIATIHQSCPS
  QEQQEILFNNVRWLAVQSRRFAPLYAVEARRISKMESTINNYIQFKSKHRIEPVCMLVHGSPGTGKGIAS
  SLIGRAIAERETTSVYSVPLAPSHFDGYKQQGYDMDDLNQNPDGMDMKLFCQMVSTVEFIPPMASLEEKG
  ILFTSDYVLASTNSHSIAPPTVAHSDALTRRFAFDVEVYTMSEHSVKGKLNMATATQLCKDCPTPANFKK
  CCPLVCGKALQLMDRYTRQRFTVDEITTLIMNEKNRRANIGNCMEALFQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001428
• ORF Size: 987 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001428.1, NP_740589
• RefSeq ORF: 987
• MW: 37.2 kDa