VP1 protein (NC_001428) Virus Tagged ORF Clone

CAT#: VC100743

Myc-DDK-tagged ORF clone of viral ORF for VP1 protein [Human enterovirus C], codon optimized for human cell expression, NP_740586

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP1 protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100743 represents NCBI reference of NP_740586 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCATTGAAGACCTTATAGACACAGCCATCAAGAATGCACTTCGCGTGTCCCAACCTCTCCGACCCA
  GCCAGTTGAAACAGCCAAACGGGGTCAATAGTCAAGAAGTCCCGGCTCTTACGGCCGTGGAGACCGGCGC
  CTCCGGGCAGGCTATCCCCTCCGATGTCGTCGAAACCAGGCATGTAATTAATTACAAGACCCGATCTGAA
  TCTTGCCTGGAATCATTTTTCGGCAGAGCCGCTTGCGTGACCATCTTGTCCCTGACAAATAGCTCTAAAA
  GTGGCGAGGAAAAAAAGCACTTCAATATTTGGAATATCACCTATACCGACACCGTGCAGCTGAGACGGAA
  ACTTGAATTTTTCACTTATAGTAGGTTCGACCTGGAGATGACATTCGTCTTCACTGAGAATTATCCAAGC
  ACTGCAAGTGGAGAAGTGAGAAATCAGTGCGATCAGATTATGTACATACCACCCGGCGCACCCAGGCCCT
  CCAGTTGGGACGACTATACTTGGCAGAGTTCCAGCAATCCCTCTATATTTTATATGTACGGGAACGCTCC
  ACCAAGGATGTCCATTCCCTATGTCGGCATCGCCAACGCCTACTCTCATTTCTATGACGGGTTTGCACGG
  GTCCCCCTCGAGGGCGAGAATACCGACGCTGGGGATACTTTTTACGGCTTGGTAAGTATAAACGATTTTG
  GCGTACTCGCAGTGAGAGCGGTGAACCGATCCAATCCACATACCATTCATACTAGTGTCCGCGTCTATAT
  GAAGCCTAAGCACATTAGGTGTTGGTGCCCTCGCCCCCCCCGCGCCGTGCTGTATAGAGGCGAAGGGGTG
  GACATGATCAGTTCTGCCATCCAACCCCTGACAAAAGTGGATTCCATTACGACCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100743 representing NP_740586
  Red=Cloning sites Green=Tags
  
  MGIEDLIDTAIKNALRVSQPLRPSQLKQPNGVNSQEVPALTAVETGASGQAIPSDVVETRHVINYKTRSE
  SCLESFFGRAACVTILSLTNSSKSGEEKKHFNIWNITYTDTVQLRRKLEFFTYSRFDLEMTFVFTENYPS
  TASGEVRNQCDQIMYIPPGAPRPSSWDDYTWQSSSNPSIFYMYGNAPPRMSIPYVGIANAYSHFYDGFAR
  VPLEGENTDAGDTFYGLVSINDFGVLAVRAVNRSNPHTIHTSVRVYMKPKHIRCWCPRPPRAVLYRGEGV
  DMISSAIQPLTKVDSITTF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001428
• ORF Size: 897 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001428.1, NP_740586
• RefSeq ORF: 897
• MW: 33.5 kDa