VP3 protein (NC_001428) Virus Tagged ORF Clone

CAT#: VC100742

Myc-DDK-tagged ORF clone of viral ORF for VP3 protein [Human enterovirus C], codon optimized for human cell expression, NP_740585

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP3 protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100742 represents NCBI reference of NP_740585 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGATTGCCCACTATGAACACACCGGGGAGCAATCAGTTTCTGACTAGCGACGATTTCCAGTCCCCTT
  GCGCATTGCCAAATTTCGATGTGACCCCCCCTATTCACATACCCGGAGAGGTGAAAAATATGATGGAGCT
  TGCAGAGATTGACACACTCATTCCCATGAACGCTGTCGACGGGAAGGTGAACACAATGGAGATGTATCAG
  ATCCCACTGAACGACAATCTCAGCAAGGCACCCATTTTCTGTCTTTCCCTGTCTCCTGCCTCCGACAAAC
  GGTTGAGCCACACTATGCTGGGAGAGATCCTGAATTACTACACCCACTGGACCGGATCAATCCGATTCAC
  GTTCTTGTTCTGTGGCTCTATGATGGCCACTGGCAAGTTGCTCCTGTCCTACTCCCCCCCGGGGGCCAAG
  CCCCCCACAAATCGGAAGGACGCTATGCTTGGGACACATATCATTTGGGACCTCGGGTTGCAGTCTTCAT
  GTTCCATGGTGGCTCCGTGGATCTCTAATACCGTTTACAGGCGGTGCGCCCGAGATGATTTTACGGAAGG
  CGGATTTATCACCTGTTTTTACCAGACCCGGATTGTCGTTCCTGCCTCCACTCCAACTTCCATGTTCATG
  CTCGGTTTCGTGTCTGCCTGCCCTGATTTCTCTGTTCGCCTGCTTCGAGACACTAGTCACATCTCACAGA
  GTAAACTGATCGCACGCACACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100742 representing NP_740585
  Red=Cloning sites Green=Tags
  
  MGLPTMNTPGSNQFLTSDDFQSPCALPNFDVTPPIHIPGEVKNMMELAEIDTLIPMNAVDGKVNTMEMYQ
  IPLNDNLSKAPIFCLSLSPASDKRLSHTMLGEILNYYTHWTGSIRFTFLFCGSMMATGKLLLSYSPPGAK
  PPTNRKDAMLGTHIIWDLGLQSSCSMVAPWISNTVYRRCARDDFTEGGFITCFYQTRIVVPASTPTSMFM
  LGFVSACPDFSVRLLRDTSHISQSKLIARTQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001428
• ORF Size: 723 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001428.1, NP_740585
• RefSeq ORF: 723
• MW: 26.7 kDa