NS1 (NC_004295) Virus Tagged ORF Clone

CAT#: VC100780

Myc-DDK-tagged ORF clone of viral ORF for NS1 protein [Human erythrovirus V9], codon optimized for human cell expression, NP_694863

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100780 represents NCBI reference of NP_694863 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTGTTTAGGGGTGTTCTGCATATCTCTTCCAATATTCTCGACTGTGCAAATGATAACTGGTGGT
  GCTCCATGTTGGATCTGGATACAAGTGATTGGGAACCACTGACTCACAGTAACCGACTGATGGCCATTTA
  TCTGTCATCTGTGGCGAGCAAGCTGGACTTCACAGGAGGCCCCCTTGCAGGCTGTCTGTATTTTTTTCAA
  GTGGAATGTAATAAATTCGAGGAGGGATACCACATCCATGTAGTGATTGGTGGACCCGGCCTGAACGCCA
  GGAACTTGACCGTCTGTGTGGAAGGCCTTTTTAACAACGTGCTCTATCATCTGGTGACTGAGTCCGTCAA
  GCTGAAGTTCCTGCCGGGAATGACCACAAAAGGCAAGTACTTCAGGGACGGCGAGCAATTCATTGAAAAC
  TATCTCATGAAAAAAATACCCCTGAATGTGGTCTGGTGTGTCACAAATATAGATGGGTACATAGACACCT
  GTATTAGCGCCTCCTTTAGACGCGGGGCTTGCCATGCAAAAAGGCCACGGATCACTGCTAATACAGACTC
  AGCAACCAATGAGACAGGTGAGTCATCTTGTGGCGGCGGTGACGTCGTTCCGTTTGCCGGAAAGGGCACC
  AAGGCTGGCCTGAAATTTCAGACAATGGTTAACTGGCTGTGCGAGAACCGCGTCTTCACGGAGGATAAAT
  GGAAATTGGTCGATTTCAATCAGTATACTCTGTTGTCAAGCAGCCATTCCGGCAGCTTCCAGATCCAGTC
  TGCTCTGAAGCTGGCGATATATAAGGCTACAAACCTGGTTCCTACTTCAACCTTCCTTCTGCATAGTGAT
  TTCGAGCAAGTTACCTGTATAAAGGAGAATAAGATCGTGAAGCTGCTGCTGTGTCAGAACTACGATCCTC
  TGCTCGTGGGACAACACGTCTTGAGGTGGATCGACAAAAAGTGCGGGAAGAAAAACACACTGTGGTTCTA
  TGGCCCCCCCTCCACCGGGAAGACCAATCTTGCCATGGCCATTGCTAAAACAGTGCCGGTTTACGGCATG
  GTTAATTGGAACAATGAGAATTTCCCCTTCAATGACGTGGCGGGAAAGAGCTTGGTCGTGTGGGACGAGG
  GAATCATCAAGTCAACGATCGTCGAGGCAGCCAAGGCAATTTTGGGTGGCCAGCCAACCCGGGTAGACCA
  GAAAATGAGAGGTTCCGTAGCCGTGCCCGGCGTCCCCGTGGTGATCACTTCTAACGGTGACATTACCTTT
  GTAGTGTCTGGCAACACCACCACCACAGTACACGCTAAGGCCCTGAAGGAGCGCATGGTGAAGCTGAACT
  TCACTATTAGGTGCAGCCCTGACATGGGCTTGCTTACGGAAGCTGATGTGCAGCAATGGCTTACGTGGTG
  CAACGCTCAATCATGGTCTCATTACGAGAACTGGGCGATCAATTACACGTTCGACTTCCCAGGAATTAAC
  GCAGACGCCTTGCATCCTGATTTGCAGACCACACCCATCGTCCCGGACACCAGCATATCTAGTTCTGGCG
  GCGAGAGCTCCGAAGAACTGTCCGAATCCTCCTTCTTCAATCTTATCACGCCTGGGGCATGGAACTCCGA
  AACACCCCGGTCCTCCACCCCAGTGCCCGGAACTAGTAGCGGAGAGAGCTTTGTAGGCTCCCCAGTGAGT
  TCAGAAGTAGTGGCCGCAAGCTGGGAGGAGGCTTTTTACACACCTCTGGCCGATCAGTTTCGCGAGCTGC
  TTGTGGGTGTCGATTTCGTCTGGGATGGTGTGAGGGGGTTGCCCGTCTGTTGTGTGGAACATATTAACAA
  TAGTGGGGGAGGGCTGGGCTTGTGTCCTCACTGTATCAACGTGGGCGCATGGTATAATGGCTGGAAATTT
  CGGGAATTCACTCCGGACTTGGTCCGGTGTTCCTGCCATGTTGGCGCTAGTAATCCATTTTCCGTGCTTA
  CTTGTAAGAAATGTGCCTATCTTAGTGGCCTCCAATCCTTTGTTGACTACGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100780 representing NP_694863
  Red=Cloning sites Green=Tags
  
  MELFRGVLHISSNILDCANDNWWCSMLDLDTSDWEPLTHSNRLMAIYLSSVASKLDFTGGPLAGCLYFFQ
  VECNKFEEGYHIHVVIGGPGLNARNLTVCVEGLFNNVLYHLVTESVKLKFLPGMTTKGKYFRDGEQFIEN
  YLMKKIPLNVVWCVTNIDGYIDTCISASFRRGACHAKRPRITANTDSATNETGESSCGGGDVVPFAGKGT
  KAGLKFQTMVNWLCENRVFTEDKWKLVDFNQYTLLSSSHSGSFQIQSALKLAIYKATNLVPTSTFLLHSD
  FEQVTCIKENKIVKLLLCQNYDPLLVGQHVLRWIDKKCGKKNTLWFYGPPSTGKTNLAMAIAKTVPVYGM
  VNWNNENFPFNDVAGKSLVVWDEGIIKSTIVEAAKAILGGQPTRVDQKMRGSVAVPGVPVVITSNGDITF
  VVSGNTTTTVHAKALKERMVKLNFTIRCSPDMGLLTEADVQQWLTWCNAQSWSHYENWAINYTFDFPGIN
  ADALHPDLQTTPIVPDTSISSSGGESSEELSESSFFNLITPGAWNSETPRSSTPVPGTSSGESFVGSPVS
  SEVVAASWEEAFYTPLADQFRELLVGVDFVWDGVRGLPVCCVEHINNSGGGLGLCPHCINVGAWYNGWKF
  REFTPDLVRCSCHVGASNPFSVLTCKKCAYLSGLQSFVDYE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_004295
• ORF Size: 2013 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_004295.1, NP_694863
• RefSeq ORF: 2013
• Locus ID: 955445
• MW: 74.0 kDa