7.5-kDa (NC_004295) Virus Tagged ORF Clone

CAT#: VC100781

Myc-DDK-tagged ORF clone of viral ORF for 75-kDa protein [Human erythrovirus V9], codon optimized for human cell expression, NP_694864

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 7.5-kDa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100781 represents NCBI reference of NP_694864 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGATGCCCAGCACTCAAATCAGTAAACCCCCCCCCCTCTCACAGACACCCGTCTCAGCTGCCGTCG
  TCGTGAAAGCACTGAAAAACTCCGTAAAGGCGGCCTTTTCAACATCAAGCCTGCAGGCGCCCGGTACCGT
  GAAGCCAAGAGCACTCGTTCGGCCTAGTCCAGGCCCCGTCCAGGAAAACCATCTGTCCGAAGCCCAATTC
  CCTCCAAAATGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100781 representing NP_694864
  Red=Cloning sites Green=Tags
  
  MQMPSTQISKPPPLSQTPVSAAVVVKALKNSVKAAFSTSSLQAPGTVKPRALVRPSPGPVQENHLSEAQF
  PPKW
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_004295
• ORF Size: 222 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_004295.1, NP_694864
• RefSeq ORF: 222
• Locus ID: 955441
• MW: 7.8 kDa