X (NC_004295) Virus Tagged ORF Clone

CAT#: VC100783

Myc-DDK-tagged ORF clone of viral ORF for X protein [Human erythrovirus V9], codon optimized for human cell expression, NP_694866

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: X
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100783 represents NCBI reference of NP_694866 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGCTACCTCACAACACCTATGCCTTACCACCCCGTGACAGTCGTTCAGAATCTCGAAGAAAAGA
  TGCAGTACTACCTGGTAAAAACTTATACCAGCCTTGGCAAATTGGCTTACAATTATCCAGTGCTCACAAT
  GCTCGGTCTGGCCATGTCCTATAAACTCGGTCTCCGCAGGATGCTCTGGACGGTGCTCCAGGGATTTATG
  ACACTGGGTATCGCTAACTGGCTGTCCTGGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100783 representing NP_694866
  Red=Cloning sites Green=Tags
  
  MDSYLTTPMPYHPVTVVQNLEEKMQYYLVKTYTSLGKLAYNYPVLTMLGLAMSYKLGLRRMLWTVLQGFM
  TLGIANWLSWE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_004295
• ORF Size: 243 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_004295.1, NP_694866
• RefSeq ORF: 243
• Locus ID: 955443
• MW: 9.5 kDa