11-kDa (NC_004295) Virus Tagged ORF Clone

CAT#: VC100785

Myc-DDK-tagged ORF clone of viral ORF for 11-kDa protein [Human erythrovirus V9], codon optimized for human cell expression, NP_694868

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 11-kDa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100785 represents NCBI reference of NP_694868 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATTGCATGACACCTCAACTGCAAATGCAGAGTAACACCACGGATACGGATATGAAGAGCCTGAAAA
  ACTGCGGCCTGCCGAAGGCCGTATGCACACACTGTAAACATTCCCCACCGTGTCCCCAACCAGGTACGGT
  TACTCACCGCCCACCTGTGCCACCTCGGCTGTACGTTCCACCCCCTATACCTAGACGACAGCCCTCTATT
  AAAGATACAGACGCTGTCGAGTATAAGCTCCTTACCCGCTACGAGCAGCATGTCATAAGAATGCTGAGGC
  TGTGCAACATGTACACATCTCTGGAAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100785 representing NP_694868
  Red=Cloning sites Green=Tags
  
  MYCMTPQLQMQSNTTDTDMKSLKNCGLPKAVCTHCKHSPPCPQPGTVTHRPPVPPRLYVPPPIPRRQPSI
  KDTDAVEYKLLTRYEQHVIRMLRLCNMYTSLEK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_004295
• ORF Size: 309 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_004295.1, NP_694868
• RefSeq ORF: 309
• Locus ID: 955440
• MW: 11.8 kDa