E1 (NC_012485) Virus Tagged ORF Clone

CAT#: VC101777

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for E1 protein [Human papillomavirus 109], codon optimized for human cell expression, YP_002756540


  View other clones from "Virus" (6)

Reconstitution Protocol

USD 780.00

3 Weeks*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Symbol E1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101777 represents NCBI reference of YP_002756540 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGACAAGGGGATCAACAGCCCCAACTCTCTGGAAGGATTCTCTGGATGGTACGTAGTGGAAGAAG
CTGAGTGCGAGGACAAGCTGACTGATCTTGAGAAGTTGTTCGATGAGAGCGATTGCTCCAATATTTCCAA
CTTGATTGACGATGGTGATGTGACCGACCAAGGCAATAGTCTGGCCCTGTTCAATCAGCAATTGCTGGAA
GATTGTGACCAGCAGCTGGTGGAGTTGAAGAGGAAGTACTTTAGCCCAGCAAAGGAAACTGATGTGGACC
TTTCTCCTCAGTTGCAGTGCGTGAGTCTGTCCGCTAGTAGCAAGAACATTAAACGGCGGCTGTTTGTTGA
TTCAGGCCTCGGGAATGAAGCAGAGGACAGTAACGAGTCAACCCAGGTGGCCGAAAGCGATTCCAATGGC
GACAAAGCTGCGGAGCAAAGTCAATCTACCGGCGATACCGATCTCAATGCTAATGGAGGCATCGTCCAGG
AGCTGCTTAAATCCCGGAACGCCACCATAACTTTTCTGGGCAAATTTAAGGAGACATTCGGCCTCTCTTA
CAAGGACCTGACTAGACCCTTCAAAAGCCACAAAACGTGTTGTCACAGCTGGGTGGCCAGTATCATCGGA
GTGCAAGATGATGTTTACGAGGCCAGTAAGACCCTGCTGCAGACACATTGCGACTTCTTTCAGGTGATTA
ACTATAGTCTGGCAAGCTACGTTTTCGTTCTCTACCTCTTTGAGTTCAAAAATAGTAAAAATCGCGAGAC
ACTCCTGAAGCTCTTGTGTCAGATTCTGTGCGTGAAGGATATACAAATCATGGCTGACCCGCCTAAACAC
AAGAATATGGTGGTAGCTCTGTATTTTTATAAGCAGGCACTGTCCTCTACCTGTTACAAGTACGGCGATT
TCCCAAATTGGTTGCTGAAGCAGACGGTGGTCCAGCACCAAAACGAGGCGGAAACTTTCATCCTCGCCAA
GATGGTTCAGTACGCCTACGACCACGACCTGGTGGACGACTCAGAGATCGCCTACCAGTATGCTCTGCTC
GCCGACGATGACCCCAACGCCGCTGCCTGGCTGAACTCTAATAATCAGGCTAAACACGTGAAAGATTGTG
CCACAATGGTCCGACATTACAAAAAATACGAAATGAGAAAAATGTCCATGTCAGAGTGGATCTTTTATTG
TTGCGATAAGGTGAAAGAAGAATCAGACTGGAAAGTGATCGCTAGGTTCCTGAAGTACCAAACGGTTAAC
TTTGTGAGCTTCCTGACAGCACTCCGCGACATGTTCAAGGGCATTCCGAAAAAACAGTGTATAGTTATCA
CCGGACCTCCCAATACCGGCAAATCTTATTTCTGTGTGAGCCTTGTCACGTTTCTGAGGGGCAGAGTGGT
TTCTTTCATGAACCATAAGTCTCACTTTTGGCTTCAGCCCCTGGCCGAAGGAAAGGTGGGATTTCTGGAT
GATGCGACCCATGCATGTTGGCAGTTCATTGACATACATATGAGAAACGGATTTGATGGGAATCCCGTTT
GCCTTGATAGCAAACATAAGAATCCTACACAGATGCGACTTCCGGCTCTGTTTATAACTACGAATGTAGA
GGTCGACAAGGAAGACCAGTACTATTATTTGAAGTCAAGGCTCCAGGTTTTCAACTTCCCTAATGTGATG
CCCCTGGACGATCTGGGAAACCCTGTGTATGAAATTACTGATAGCAGTTGGAATTCTTTCTTCAGGCGCT
TGGAAAAGCAGTTGGATATTTGCAGACCGACTGAAAGCAACGATGGCGACCCCGACAGGGCCTTCAGATG
TTTTAGGGGTGAGTCAGCCTCTACTATC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101777 representing YP_002756540
Red=Cloning sites Green=Tags

MADKGINSPNSLEGFSGWYVVEEAECEDKLTDLEKLFDESDCSNISNLIDDGDVTDQGNSLALFNQQLLE
DCDQQLVELKRKYFSPAKETDVDLSPQLQCVSLSASSKNIKRRLFVDSGLGNEAEDSNESTQVAESDSNG
DKAAEQSQSTGDTDLNANGGIVQELLKSRNATITFLGKFKETFGLSYKDLTRPFKSHKTCCHSWVASIIG
VQDDVYEASKTLLQTHCDFFQVINYSLASYVFVLYLFEFKNSKNRETLLKLLCQILCVKDIQIMADPPKH
KNMVVALYFYKQALSSTCYKYGDFPNWLLKQTVVQHQNEAETFILAKMVQYAYDHDLVDDSEIAYQYALL
ADDDPNAAAWLNSNNQAKHVKDCATMVRHYKKYEMRKMSMSEWIFYCCDKVKEESDWKVIARFLKYQTVN
FVSFLTALRDMFKGIPKKQCIVITGPPNTGKSYFCVSLVTFLRGRVVSFMNHKSHFWLQPLAEGKVGFLD
DATHACWQFIDIHMRNGFDGNPVCLDSKHKNPTQMRLPALFITTNVEVDKEDQYYYLKSRLQVFNFPNVM
PLDDLGNPVYEITDSSWNSFFRRLEKQLDICRPTESNDGDPDRAFRCFRGESASTI

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_012485
ORF Size 1848 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Reference Data
RefSeq NC_012485.1, YP_002756540
RefSeq ORF 1848
Locus ID 7701329
MW 70.4 kDa

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