E1 (NC_012485) Virus Tagged ORF Clone

CAT#: VC101777

Myc-DDK-tagged ORF clone of viral ORF for E1 protein [Human papillomavirus 109], codon optimized for human cell expression, YP_002756540

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101777 represents NCBI reference of YP_002756540 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGACAAGGGGATCAACAGCCCCAACTCTCTGGAAGGATTCTCTGGATGGTACGTAGTGGAAGAAG
  CTGAGTGCGAGGACAAGCTGACTGATCTTGAGAAGTTGTTCGATGAGAGCGATTGCTCCAATATTTCCAA
  CTTGATTGACGATGGTGATGTGACCGACCAAGGCAATAGTCTGGCCCTGTTCAATCAGCAATTGCTGGAA
  GATTGTGACCAGCAGCTGGTGGAGTTGAAGAGGAAGTACTTTAGCCCAGCAAAGGAAACTGATGTGGACC
  TTTCTCCTCAGTTGCAGTGCGTGAGTCTGTCCGCTAGTAGCAAGAACATTAAACGGCGGCTGTTTGTTGA
  TTCAGGCCTCGGGAATGAAGCAGAGGACAGTAACGAGTCAACCCAGGTGGCCGAAAGCGATTCCAATGGC
  GACAAAGCTGCGGAGCAAAGTCAATCTACCGGCGATACCGATCTCAATGCTAATGGAGGCATCGTCCAGG
  AGCTGCTTAAATCCCGGAACGCCACCATAACTTTTCTGGGCAAATTTAAGGAGACATTCGGCCTCTCTTA
  CAAGGACCTGACTAGACCCTTCAAAAGCCACAAAACGTGTTGTCACAGCTGGGTGGCCAGTATCATCGGA
  GTGCAAGATGATGTTTACGAGGCCAGTAAGACCCTGCTGCAGACACATTGCGACTTCTTTCAGGTGATTA
  ACTATAGTCTGGCAAGCTACGTTTTCGTTCTCTACCTCTTTGAGTTCAAAAATAGTAAAAATCGCGAGAC
  ACTCCTGAAGCTCTTGTGTCAGATTCTGTGCGTGAAGGATATACAAATCATGGCTGACCCGCCTAAACAC
  AAGAATATGGTGGTAGCTCTGTATTTTTATAAGCAGGCACTGTCCTCTACCTGTTACAAGTACGGCGATT
  TCCCAAATTGGTTGCTGAAGCAGACGGTGGTCCAGCACCAAAACGAGGCGGAAACTTTCATCCTCGCCAA
  GATGGTTCAGTACGCCTACGACCACGACCTGGTGGACGACTCAGAGATCGCCTACCAGTATGCTCTGCTC
  GCCGACGATGACCCCAACGCCGCTGCCTGGCTGAACTCTAATAATCAGGCTAAACACGTGAAAGATTGTG
  CCACAATGGTCCGACATTACAAAAAATACGAAATGAGAAAAATGTCCATGTCAGAGTGGATCTTTTATTG
  TTGCGATAAGGTGAAAGAAGAATCAGACTGGAAAGTGATCGCTAGGTTCCTGAAGTACCAAACGGTTAAC
  TTTGTGAGCTTCCTGACAGCACTCCGCGACATGTTCAAGGGCATTCCGAAAAAACAGTGTATAGTTATCA
  CCGGACCTCCCAATACCGGCAAATCTTATTTCTGTGTGAGCCTTGTCACGTTTCTGAGGGGCAGAGTGGT
  TTCTTTCATGAACCATAAGTCTCACTTTTGGCTTCAGCCCCTGGCCGAAGGAAAGGTGGGATTTCTGGAT
  GATGCGACCCATGCATGTTGGCAGTTCATTGACATACATATGAGAAACGGATTTGATGGGAATCCCGTTT
  GCCTTGATAGCAAACATAAGAATCCTACACAGATGCGACTTCCGGCTCTGTTTATAACTACGAATGTAGA
  GGTCGACAAGGAAGACCAGTACTATTATTTGAAGTCAAGGCTCCAGGTTTTCAACTTCCCTAATGTGATG
  CCCCTGGACGATCTGGGAAACCCTGTGTATGAAATTACTGATAGCAGTTGGAATTCTTTCTTCAGGCGCT
  TGGAAAAGCAGTTGGATATTTGCAGACCGACTGAAAGCAACGATGGCGACCCCGACAGGGCCTTCAGATG
  TTTTAGGGGTGAGTCAGCCTCTACTATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101777 representing YP_002756540
  Red=Cloning sites Green=Tags
  
  MADKGINSPNSLEGFSGWYVVEEAECEDKLTDLEKLFDESDCSNISNLIDDGDVTDQGNSLALFNQQLLE
  DCDQQLVELKRKYFSPAKETDVDLSPQLQCVSLSASSKNIKRRLFVDSGLGNEAEDSNESTQVAESDSNG
  DKAAEQSQSTGDTDLNANGGIVQELLKSRNATITFLGKFKETFGLSYKDLTRPFKSHKTCCHSWVASIIG
  VQDDVYEASKTLLQTHCDFFQVINYSLASYVFVLYLFEFKNSKNRETLLKLLCQILCVKDIQIMADPPKH
  KNMVVALYFYKQALSSTCYKYGDFPNWLLKQTVVQHQNEAETFILAKMVQYAYDHDLVDDSEIAYQYALL
  ADDDPNAAAWLNSNNQAKHVKDCATMVRHYKKYEMRKMSMSEWIFYCCDKVKEESDWKVIARFLKYQTVN
  FVSFLTALRDMFKGIPKKQCIVITGPPNTGKSYFCVSLVTFLRGRVVSFMNHKSHFWLQPLAEGKVGFLD
  DATHACWQFIDIHMRNGFDGNPVCLDSKHKNPTQMRLPALFITTNVEVDKEDQYYYLKSRLQVFNFPNVM
  PLDDLGNPVYEITDSSWNSFFRRLEKQLDICRPTESNDGDPDRAFRCFRGESASTI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012485
• ORF Size: 1848 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012485.1, YP_002756540
• RefSeq ORF: 1848
• Locus ID: 7701329
• MW: 70.4 kDa