L2 (NC_012485) Virus Tagged ORF Clone

CAT#: VC101780

Myc-DDK-tagged ORF clone of viral ORF for L2 protein [Human papillomavirus 109], codon optimized for human cell expression, YP_002756543

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101780 represents NCBI reference of YP_002756543 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTCAGATATTCCCCGCAAGCGAACTAAACGGGATTCTGCGACCAACCTGTACAATCAGTGTCGAA
  TAACTGGTAATTGTCCGGAGGACGTGAAAAATAAAATAGAGGGGAATACTCTTGCCGATAAGCTTCTCAA
  GATTCTGAGCAGCATTGTCTACTTCGGCGGTTTGGGCATCGGAACGGGCCGCGGGACGGGAGGTGCAACG
  GGATATAGACCTTTGGGAGGGGGAGGCGGCGGCGGTGGCGGGCGAGTAACTACTGACGGCATTGTGATCC
  GACCTAACATCGTAGTGGAACCTGTGGGACCTTCTGAGCTGGTGCCAATTGACGCCCTCAACCCCTCCTC
  CTCATCTGTCGTGCCTATGGTAGAGGTTGGACCTGAAATTGTCGTTCCCGATGCGATACCTGATATCAAC
  ACGGGCGAGCTCGATATCATCACACAGCCCGACATCATCGACGTGACAGGGCCACGCTCAACACCCGCTG
  TATCCACCACCGCCGACGACACTGCCGCGATAATCGAGGTGCAGCCGGCAGCCCCTATTCCGAGGCGAGT
  CGCCACCAGCACTAGCCGCTTTTCCAACCCAACATTCGTCGCCACGACCGACACAGTTCTCCCTGAAACC
  ACAAGCCTGGGGAATAACGTGTTCATCGATGCGGCCACCGGCGGTGAAGTTATCGGTGAAGAGATCCCCC
  TGGACACCTTCAACGAGCCTGAGGAATTCAATATTGAAGAACCAGCCTACCCTCGGAGTTCCACCCCAAG
  GGCATTTTCACGGGCTTTCTTTCGCGCACGGGAGCTCTACAATAGAAGGGTTCGGCAGGTCATGACCCAG
  AACTTGAATTTTCTGACTCGGGCACCCCAGGCTATTCAATTTAATTTCGAGAATCCTGCATTTGACAACG
  ACATCACGCTTGAGTTCGAACAAGATGTTAATCAGCTGGCTGCCGCCGCCCCCGACCCAGACTTTGCGGA
  TATCATCAAACTGCATAGGCCTGTTTTTAGTGAGACGGTCGGAGGAAATATTCGGTTGTCACGGCTCGGC
  CAGAAGGGGACTATTCGCCTGAGGTCAGGGACCCAGATTGGTGAGACAGTGCATCTGTATTATGACATCT
  CTTCTATAGAGAACGCCGATGCGATCGAGCTGTCCGTGCTGGGAGAGCATAGCGGAGACGCGACACTCAT
  CAATCCTGTGGCCGAGAGCACGTTTGTGGATGCTGAGAATTCAGACGCCCCCCTCCTGTTCCCTGAAGAA
  GAACTGGTGGACGATCTGACCGAGGATTTCTCTAATAGTCATGTGGTACTGAATACTGGATCCAGGCGCA
  GTATTCTTAACGTACCAACCTTGCCGAGAGGCGCCGCCCTTAAGGTGTTTATCGACGATGTGGGGGACGG
  TCTCTTTGTGAGTCACCCGACAAGTACCGACATGATTCCAGATAATTTCATCCCCGCTTTCGACGTCGGC
  CCAAGCATCTTGATTAATGAGAGTTCAAGCGACGACTTCGTTCTGCACCCAAGCCATATCCCTCGACGAA
  AGCGCAAGCGCGCCTACCCGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101780 representing YP_002756543
  Red=Cloning sites Green=Tags
  
  MTSDIPRKRTKRDSATNLYNQCRITGNCPEDVKNKIEGNTLADKLLKILSSIVYFGGLGIGTGRGTGGAT
  GYRPLGGGGGGGGGRVTTDGIVIRPNIVVEPVGPSELVPIDALNPSSSSVVPMVEVGPEIVVPDAIPDIN
  TGELDIITQPDIIDVTGPRSTPAVSTTADDTAAIIEVQPAAPIPRRVATSTSRFSNPTFVATTDTVLPET
  TSLGNNVFIDAATGGEVIGEEIPLDTFNEPEEFNIEEPAYPRSSTPRAFSRAFFRARELYNRRVRQVMTQ
  NLNFLTRAPQAIQFNFENPAFDNDITLEFEQDVNQLAAAAPDPDFADIIKLHRPVFSETVGGNIRLSRLG
  QKGTIRLRSGTQIGETVHLYYDISSIENADAIELSVLGEHSGDATLINPVAESTFVDAENSDAPLLFPEE
  ELVDDLTEDFSNSHVVLNTGSRRSILNVPTLPRGAALKVFIDDVGDGLFVSHPTSTDMIPDNFIPAFDVG
  PSILINESSSDDFVLHPSHIPRRKRKRAYPF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012485
• ORF Size: 1563 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012485.1, YP_002756543
• RefSeq ORF: 1563
• Locus ID: 7701332
• MW: 56.2 kDa