E2 (NC_012485) Virus Tagged ORF Clone

CAT#: VC101778

Myc-DDK-tagged ORF clone of viral ORF for E2 protein [Human papillomavirus 109], codon optimized for human cell expression, YP_002756541

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101778 represents NCBI reference of YP_002756541 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGAAACGTTGACCGAACGGTTCGACGCCCTGGAAGAGAACCTCCTCCAATTGTATGAGGCCGGAT
  CCGATAAGATTGACGATCAGATTCTGTACTGGGACATTATTCGCCAGGAAAACGTTCTTTTCTTCTACGC
  TAGGAAGAAGGGCCTTCAGAGGATTTCCCTGCAGCCAGTTCCCAGCCTGACCGTCTCCGAACATAAGGCT
  AAACAAGCTATCATGATGAGTATACAGTTGACTAGCCTGAAGAACAGCGCTTTCGGTAACGAACCTTGGA
  CTCTGCAGGACACTTCCTTTGAGCTCTATAATGCGCCACCGCAGAATACATTTAAAAAACAGGCTTTTAC
  TGTGGATGTTTTTTATGACAACGACGAGGACAATTATTACCCGTATACTGCCTATAAATTTATTTATTAC
  CAGAATGGGGATAACATCTGGCATAAAGTGGAGAGCGACGTGGACTACGAGGGTCTTTTCTACATAACTC
  ATGACTATGAAAAAGTGTATTACGTGACATTCGACCAGGACGCTCGGCGGTTTTCCAGAACCGGGCACTG
  GACTGTTAAGTACAAGAATCGAACCATCTCATCCACCTCAGTGACAAGCACCTCTGGCACCCCACCTGAA
  CATCCCAGTTCCAGTCGCTCTGGTGCCAACACATTGGCTAGACAGACTGAGATAGCCCCACAGGAACGCA
  GACGGCCCCGATCCAGTTCAGAAAGCCCTCAGAGACATACTCGCCCTCGAACCTCCCAGTCCGACGGGGA
  TACTAGTGCGTCAACCCCCCGGCGGAGGCTCAGACGAAGAAGAAGAGAGAGGGAGCCAGCCGCACAGCGC
  AGAGAACAGAGAAAGACTAATACCACTCGGTCCTCCTACCCAAGCCCGGAGGCAGTCGGACGCAGGCATA
  GGGTTGTGGAGGGTAAGGGACTGTCTCGGCTCGGAAGGTTGCAGGAGGAAGCACGAGGTCCACCTCTGAT
  CTGTCTGAAGGGACCTGCAAACACCCTGAAGTGTTGGAGGTATCGGTGTAAACTGAAATATCGCGGCATG
  TTCTACAGGATCAGTACCGGATTCAGCTGGGTGGCTGAAGGTCCAGCTCGCCTGGGCGACCAACGAATGT
  TGATCGCTTTTCACAACTCTGCTCAGCGCCAAGCCTTCCTCAAGATCGCCGCGTTTCCTAAAGGGACCCA
  ATTCGCGCTCGGAAATCTGGATTCACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101778 representing YP_002756541
  Red=Cloning sites Green=Tags
  
  MMETLTERFDALEENLLQLYEAGSDKIDDQILYWDIIRQENVLFFYARKKGLQRISLQPVPSLTVSEHKA
  KQAIMMSIQLTSLKNSAFGNEPWTLQDTSFELYNAPPQNTFKKQAFTVDVFYDNDEDNYYPYTAYKFIYY
  QNGDNIWHKVESDVDYEGLFYITHDYEKVYYVTFDQDARRFSRTGHWTVKYKNRTISSTSVTSTSGTPPE
  HPSSSRSGANTLARQTEIAPQERRRPRSSSESPQRHTRPRTSQSDGDTSASTPRRRLRRRRREREPAAQR
  REQRKTNTTRSSYPSPEAVGRRHRVVEGKGLSRLGRLQEEARGPPLICLKGPANTLKCWRYRCKLKYRGM
  FYRISTGFSWVAEGPARLGDQRMLIAFHNSAQRQAFLKIAAFPKGTQFALGNLDSL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012485
• ORF Size: 1218 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012485.1, YP_002756541
• RefSeq ORF: 1218
• Locus ID: 7701330
• MW: 47.1 kDa