E4 (NC_012485) Virus Tagged ORF Clone

CAT#: VC101779

Myc-DDK-tagged ORF clone of viral ORF for E4 protein [Human papillomavirus 109], codon optimized for human cell expression, YP_002756542

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101779 represents NCBI reference of YP_002756542 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGAGGGCCTGGCCGGACAGGATATCGGCCTGTTGAACATCAAGATAGTTCCTTTCTTGCTGCTGC
  TTTTGCCAGCTCTCGCTGGTCCTTTGCCCTCTACACCCAGACCACCGGGCCGAGCTCCCACACCTTGGTT
  GGATAAACCAAAAAGCCATCACAAACGCGATGAGGATCCAGATCATCCTCAACGGGTGCCCTCCGGGATA
  CTTGATAGGGTACGGCGAAAAGCTACTGGCACCCCAGTACGACGGCGGCTTGAGGATGACTTTGACGACG
  ACGACGAGAAGGAGAACCAGCCTCCTAACGGTGAGAATAACGAGAAACCAATCCCACCCGATCACCTGAC
  AAGACTCCTCAGACAGTGGGGTGAGGACATCGAGTGGCTGAAGGAAAAGGTATCTCAAGATCTTGACGAC
  TACAAAAAGCGCCTTGGCGTTCCACCTCTGTTCGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101779 representing YP_002756542
  Red=Cloning sites Green=Tags
  
  MLEGLAGQDIGLLNIKIVPFLLLLLPALAGPLPSTPRPPGRAPTPWLDKPKSHHKRDEDPDHPQRVPSGI
  LDRVRRKATGTPVRRRLEDDFDDDDEKENQPPNGENNEKPIPPDHLTRLLRQWGEDIEWLKEKVSQDLDD
  YKKRLGVPPLFA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012485
• ORF Size: 456 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012485.1, YP_002756542
• RefSeq ORF: 456
• Locus ID: 7701331
• MW: 17.3 kDa