L1 (NC_012485) Virus Tagged ORF Clone

CAT#: VC101781

Myc-DDK-tagged ORF clone of viral ORF for L1 protein [Human papillomavirus 109], codon optimized for human cell expression, YP_002756544

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101781 represents NCBI reference of YP_002756544 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCATATTGGCTGCCCGCTTCTGGTAAAGTCTACCTGCCTCCCTCAAGACCCGTTGCAAGAGTCCTTA
  GTACCGATGATTACGTTCAAGAGACCAGTCTGTTTTTCCATGCTAGTTCCGATCGGCTTCTTACCGTGGG
  ACACCCCTACTTTCCTGTCAAGGATCCGATTACAAAGGCAATCAATGTGCCCAAAGTGTCTGGAAATCAG
  TTTCGCGTTTTCCGGCTGCAGTTCCCTGACCCGAATCGATTCGCCCTGGTAGATCCCTCAGTGTATAATC
  CAGACACTGAGAGACTTGTCTGGAAACTCAGAGGAATAGAAGTCATTAGAGGCGGACCTCTGGGAATTGG
  CTCTACAGGCCATCCACTGTTCAACAAGCTGAATGATACTGAGAACCCAAATAATTACCTGAAGGGAAGC
  ACCGATAATAGACAGAATGTCTCACTGGATCCTAAACAAACCCAACTTTTGATTGTCGGCTGTGTGCCAT
  GTACCGGTGCACACTGGGATGCTGCCAAGGCCTGCGCTGAGCCTGTACCCAAAAAGGGCGACTGCCCCCC
  TCTGCAGCTGATCAATAGTATCATTGAGGATGGAGATATGTGTGACATTGGTTTCGGAGCCATGAACTTC
  AACGCCCTGCAACAGGATCGGTCCGGAGCTCCCTTGGACATCGTCGCTACTACCTGTAAGTGGCCGGACC
  TGGTCAAGATGTCCAACAACGTGTATGGCGACGATTTGTTCTTTTTCGGGAAAAGGGAGCAGGAATATGC
  CCGACACTATTTCACTCGGCATGGGGTTGTTGGAGATAGCATTCCCCAGGTTAATGAAGATCCCCAGACA
  TTGTATGTTAGACCTGGCAAATCCGGGCAACAACAGAATACAGTAAGTAGTCCCGTGTATTTCGCAACAC
  CCAGCGGATCCTTGGTTACTAGTGATGCACAAATCCTGAATCGGCCTTACTGGATGCAGCGGGCACAAGG
  AATGAACAACGGAGTCTGCTGGAACAACAATTTGTTTGTGACTGTCGTTGACAACACCCATAATACAAAC
  TTTACAATCTCTCAATACAGCGGAGCTCAAGAGCAGCCCCCACAAGAATATGACTCAAGCGACTATAAAG
  TTTATCTGCGCCACGTGGAAGAGTTTGACATAAGCGTCATCGTGCAAATCTGTAAGATATCCCTGGATGC
  AGACATCCTGGCTCATTTGAATACTATGAATCCTACCATCCTCGAGAATTGGAACCTGGCCTTCGTCCCC
  CCCCCTGCCAGTGGTATTGAAGATCATTATCGCTATATTAACAGCCTTGCCACAAGGTGCCCAGATCAGA
  ACCCACCACCAGAGAAAGAAGATCCTTACGCCAAATATAACTTCTGGGCCGTCGATATGACGGACAAAAT
  GTCAAACGACTTGACGCAGACCAGTCTGGGGAGACGATTTGTGTACCAGATAGGGCTGGCCGCCCGCACA
  AATTCACTTACAGGCAAGCGGAAAAGAGTGGCAATAGGCTCTAGTAATACCAGCAAAAGTTCTAAGCGCA
  AACGGAAATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101781 representing YP_002756544
  Red=Cloning sites Green=Tags
  
  MSYWLPASGKVYLPPSRPVARVLSTDDYVQETSLFFHASSDRLLTVGHPYFPVKDPITKAINVPKVSGNQ
  FRVFRLQFPDPNRFALVDPSVYNPDTERLVWKLRGIEVIRGGPLGIGSTGHPLFNKLNDTENPNNYLKGS
  TDNRQNVSLDPKQTQLLIVGCVPCTGAHWDAAKACAEPVPKKGDCPPLQLINSIIEDGDMCDIGFGAMNF
  NALQQDRSGAPLDIVATTCKWPDLVKMSNNVYGDDLFFFGKREQEYARHYFTRHGVVGDSIPQVNEDPQT
  LYVRPGKSGQQQNTVSSPVYFATPSGSLVTSDAQILNRPYWMQRAQGMNNGVCWNNNLFVTVVDNTHNTN
  FTISQYSGAQEQPPQEYDSSDYKVYLRHVEEFDISVIVQICKISLDADILAHLNTMNPTILENWNLAFVP
  PPASGIEDHYRYINSLATRCPDQNPPPEKEDPYAKYNFWAVDMTDKMSNDLTQTSLGRRFVYQIGLAART
  NSLTGKRKRVAIGSSNTSKSSKRKRKS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012485
• ORF Size: 1551 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012485.1, YP_002756544
• RefSeq ORF: 1551
• Locus ID: 7701333
• MW: 57.9 kDa