E7 (NC_012486) Virus Tagged ORF Clone

CAT#: VC101783

Myc-DDK-tagged ORF clone of viral ORF for E7 protein [Human papillomavirus 112], codon optimized for human cell expression, YP_002756546

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101783 represents NCBI reference of YP_002756546 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTGGAAACCAGCCGACTGCCGAGAATTTGGAGATCAATCTGGAAGAATTGGTTCTTCCCGAGAACT
  TGCTCGCGGAAGAATCTTTGAGCCCAGATGTGGACCCTGAAGAAGAAGAGAGACAGCCCTTCTGGGTAGA
  CACTTGCTGTGGCACCTGCAACGCTTCCGTACGGCTCAGCATCTTGGCCACACAGTCCGCCGTTTGCCTG
  ATGAGAGTACTGCTGCAGGGAGAGCTGAGTTTCGTATGTATCCGATGCTCAAAGGATCGCTTGCGACATG
  GCAGAACAAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101783 representing YP_002756546
  Red=Cloning sites Green=Tags
  
  MIGNQPTAENLEINLEELVLPENLLAEESLSPDVDPEEEERQPFWVDTCCGTCNASVRLSILATQSAVCL
  MRVLLQGELSFVCIRCSKDRLRHGRTS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012486
• ORF Size: 291 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012486.1, YP_002756546
• RefSeq ORF: 291
• Locus ID: 7701319
• MW: 10.8 kDa