L2 (NC_012486) Virus Tagged ORF Clone

CAT#: VC101787

Myc-DDK-tagged ORF clone of viral ORF for L2 protein [Human papillomavirus 112], codon optimized for human cell expression, YP_002756550

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101787 represents NCBI reference of YP_002756550 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACAAGGCGAAGCGGGTGAAACGGGATACAGTGGAGAACATTTACCGCCAATGTAAAATTTCCGGCA
  CATGTCCCGATGACGTAGTGAATAAGGTGGAACAAAACACACTGGCCGACATCCTCTTGAAAATTTTTGG
  CTCAGTAATCTACCTCGGTGGCCTTGGTATAGGGTCCGGATCCGGAGCTGGGGCTACAGGCTTCCGCCCA
  ATAACTGAGAACATACCACTGGAAACACTTCCACCTATTGACCCAATCCCAGATTCCGACATCCCAGTGG
  TCAGGCCCAGCAGACCAACACGCCCTTTCGGTAGCAACACCTTCGGTACACGGATCGACCCTATCGGATC
  CGCTGCAAATCGCCCCCGACCCGTCGACCCACAGGGGCCTGCGATCGTACCTTTGAACGAGGGAGGTCTC
  CCCGATAGCACGATCATCTCTACGGGCGCCGGCCCAGGAACGGGAATTTCAGACTACGAAATCCTGACTA
  CCACGGACGTCTTTGAAGACCTGTCTACGGTTAACGGCCACCCAACCGTGCTGCACGGCCAGGAAGACAT
  CGCTATCCTCCAGGTCACTCCACCAGATCCAATACCCCCTCGCCTTGCTTTTGAAAACCCAAGAGAGGCT
  AATGCCGTTGTGATCGAGTCTAGTGTGCCACCACCGGAGTTCCTGAACGTGTACGTGGATCCCAGCTACA
  CCGGAATCCAGGTTGGAGAGGAAATTGAGTTGGAACCCATCAATACAATAGCCGAGTTTGAAATTGAAGA
  GAATATTCCCAGGACCTCCACCCCTGCCCGGGTTCTGGACTCAGCTGTTAAGAGGGTGCGGGGCCTTTAC
  AATCGGATCGTTGACCAAGTCGCCACCAGGAATATTGATTTTCTTGGACAGCCGAGTCGAGCAATCCTTT
  TTGAATACGACAATCCTGCATTTGATAACGACATTACATTGACATTCGAGCGCGACCTCCAGGAAGTTGC
  AGCGGCTCCTGACGCAGCCTTTACAGACGTGATCCAATTGGACAGGCCGATTTATTCTGAGACCAAGGAG
  GGACTGGTGCGGTTTTCCAGACTGGGGACGAAAGGCAAAATCAGCACCAGGTCAGGAAACATACTGACCC
  AGAAGGTCCATTACCATTATGATATCAGCCCGATTTCCACAATCGTCGAGGAGATTGAGTTGCAACCTAT
  CCATGACACTTCTGACATGCTCACAATCGTCGACGAGCTCAGCCATAACACATTCATCAATCCGGTCTTT
  GAGGATAACTTTCCAATTGAAACACTGGACGATGACTTTACCGAATCATTCGCTGATAGTCAGCTGGCAA
  TCTTGGACGCAGAGGAGAATGAAAACATTATTGTGCCCACTCTGGTATCAGACATCTCCCCTAAGGTGGT
  AAGCTCTGATTATTTCAGCACATTCACTTCAATCGGCACAACCACGTACCCTGACATCCCACTGAACACT
  AATAGTATCCCATACACCCCAGTGATCTACATCGACCCCTTCGGGTCAGATTTCTATTTGCATCCTGCCC
  TTCTGAAGCGGCGAAAAAGGAAATACTCTGAAATATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101787 representing YP_002756550
  Red=Cloning sites Green=Tags
  
  MYKAKRVKRDTVENIYRQCKISGTCPDDVVNKVEQNTLADILLKIFGSVIYLGGLGIGSGSGAGATGFRP
  ITENIPLETLPPIDPIPDSDIPVVRPSRPTRPFGSNTFGTRIDPIGSAANRPRPVDPQGPAIVPLNEGGL
  PDSTIISTGAGPGTGISDYEILTTTDVFEDLSTVNGHPTVLHGQEDIAILQVTPPDPIPPRLAFENPREA
  NAVVIESSVPPPEFLNVYVDPSYTGIQVGEEIELEPINTIAEFEIEENIPRTSTPARVLDSAVKRVRGLY
  NRIVDQVATRNIDFLGQPSRAILFEYDNPAFDNDITLTFERDLQEVAAAPDAAFTDVIQLDRPIYSETKE
  GLVRFSRLGTKGKISTRSGNILTQKVHYHYDISPISTIVEEIELQPIHDTSDMLTIVDELSHNTFINPVF
  EDNFPIETLDDDFTESFADSQLAILDAEENENIIVPTLVSDISPKVVSSDYFSTFTSIGTTTYPDIPLNT
  NSIPYTPVIYIDPFGSDFYLHPALLKRRKRKYSEIF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012486
• ORF Size: 1578 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012486.1, YP_002756550
• RefSeq ORF: 1578
• Locus ID: 7701323
• MW: 57.9 kDa