E2 (NC_012486) Virus Tagged ORF Clone

CAT#: VC101785

Myc-DDK-tagged ORF clone of viral ORF for E2 protein [Human papillomavirus 112], codon optimized for human cell expression, YP_002756548

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101785 represents NCBI reference of YP_002756548 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCGAGACCCAGGAAACCCTTACACAACGCTTCAACGCTTTGCAGGAAGAAATCTTGAATATAATTG
  AAGAGGGTCCAACCGATCTGCAGAGCATTATTAAATTCTGGCAGCTGTCTCGGAAGGAGAATGTTATCCT
  GTACTATGCAAGGAAAGAAAATTATACCACCCTGGGCCTGCAGCCCGTACCGGCCCTGGCCGTGTCCGAG
  TACAAGGCTAAAGAAGCTATCCAGATCCAGCTGTTGGTGACAAGCCTGTCCAAGTCTGCTTACGCCGCCG
  AAACCTGGACTCTCCAGGACTGCTCTGCAGAGCTGCTGAACACACAGCCCAAAAATTGTTTTAAGAAAGC
  TGGCTATTCAGTTGATGTGTGGTTCGATCATGACAAGGCGAAGGCTTTCCCCTATACTAATTGGAAATAC
  ATTTATTACCAGGATGCCAGCGATACATGGCACAAAGTGAATGGTAAAGTGGATGCAAATGGACTCTACT
  ATGATGAGATCAACGGGGATCGCGTGTATTTCACTCTGTTCGAAGCTGATAGCACAAAGTACGGGCTGAC
  CGGTGAGTGGACCGTTCACTATGAAAACACAACCATCGTCTCTTCAAGCAGCTCAAATCGCCGAATCGGG
  CAGAGCCAGAAATTCGCCGATTCTACATCTTCACGGGACAGTACAACACCATCACCTAAGAGACGCCGAA
  TCACACAGGAAGCTGATTCTGCCACACCCAGTACTTCAACTGACAGTATCGCCACAGTCTCCCTGCGGGC
  AGGCGGGCGGAGACAGCAAAGAAAACCTACATCTACAGTCTCTACTCGGCGCAGAACGCGCTCACCACAG
  AGATCAGCTGCTCCCACACCGTCCGAGGTGGGTACCGGCCATCGATTGCCACCGCGCCAGGGACTCGGAC
  GCCTTCAGCGCCTGCAAGCCGAGGCATGGGATCCATACCTCCTTATTCTCAAGGGACCAAGCAACAATCT
  CAAGTGTTGGAGGAACAAAGTGAAACTGCAGAACTGGTTTCGAGATAGCAGTAATGTGTGGCGGTGGCTG
  GGGCCAGAGACAACTCAGAGCAGGATGCTCCTGGCGTTTGACAGTACGATTCAGCGCGAAAGGTTCCTGC
  TGCACGTCAAACTTCCAAAAAACACTACGTTTGCGTACGGTTCTTTGGACTCCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101785 representing YP_002756548
  Red=Cloning sites Green=Tags
  
  MVETQETLTQRFNALQEEILNIIEEGPTDLQSIIKFWQLSRKENVILYYARKENYTTLGLQPVPALAVSE
  YKAKEAIQIQLLVTSLSKSAYAAETWTLQDCSAELLNTQPKNCFKKAGYSVDVWFDHDKAKAFPYTNWKY
  IYYQDASDTWHKVNGKVDANGLYYDEINGDRVYFTLFEADSTKYGLTGEWTVHYENTTIVSSSSSNRRIG
  QSQKFADSTSSRDSTTPSPKRRRITQEADSATPSTSTDSIATVSLRAGGRRQQRKPTSTVSTRRRTRSPQ
  RSAAPTPSEVGTGHRLPPRQGLGRLQRLQAEAWDPYLLILKGPSNNLKCWRNKVKLQNWFRDSSNVWRWL
  GPETTQSRMLLAFDSTIQRERFLLHVKLPKNTTFAYGSLDSL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012486
• ORF Size: 1176 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012486.1, YP_002756548
• RefSeq ORF: 1176
• Locus ID: 7701321
• MW: 44.8 kDa