L1 (NC_012486) Virus Tagged ORF Clone

CAT#: VC101788

Myc-DDK-tagged ORF clone of viral ORF for L1 protein [Human papillomavirus 112], codon optimized for human cell expression, YP_002756551

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101788 represents NCBI reference of YP_002756551 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCTTGTGGCTCCAGCAATCCGGAACCATTTACCTGCCTCCCGCAAAGCCTGTGGCGAAAGTTCTCT
  CTACCGACGAGTATGTCATCGGGACCAATCTTTTCTTTTATGCGTCCTCTGAGAGGCTGCTCACAGTAGG
  GCACCCATACTTCGAGATTCCAAACCCCGCGCTGCCTGGCGCATTGGCCGTGCCCAAAGTGAGCGGTAGC
  CAATACCGCGTGTTTAGATGCATGCTGCCGGACCCCAACAAATTTGCGCTGATCGAGCGGTCATTCTATA
  ATCCTGAAAGGGAGCGGCTGGTTTGGAAAGTAAGAGGGCTCCAGCTGGGCAGAGGGGGGCCTCTCGGGCT
  CGGAACTTCAGGACACCCATTGTTTAATAAGGTAGCTGATACAGAGAACCCCACCACATACCCCGCGGCC
  GAAACTGACGAGCAAAGAGTGAACCTGTCTATGGATCCCAAGCAGGTGCAGCTCCTGATCGTGGGTTGCG
  AGCCTGCATTGGGAGAACATTGGGACGTTGCTAAACCTTGCTCTGACGAAGTGAACCAGAACCAGGGGGA
  CTGCCCCCCAATTCAGTTGCTCAATACTGTCATTCAAGACGGCGACATGTGTGATATCGGCTTTGGGGCC
  GCGAACTTCAAAACCTTGCAGCAGGACAAATCAGGCGTCCCACTCGACATCGTCGACTCTATTTGCAAAT
  GGCCGGATCTCATTAAGATGGAGCAGAACATTTATGGCGATAACTTGTTCTTTTTCACTAAGAAGGAACA
  GGCTTACGCCCGGCACTATTTTGCTCGAGCGGGAACCAATGGGGACCCGATCCCAGATGGCACCACTCCT
  AATCAGGATGCTTTTTACCTCTCACCCGACCAGGCAGGCCAAGCCCCACAAAAGACGTTGGCTAGCTATA
  CCTATTTCCCCACAGTCAGCGGGTCACTGGTGTCTTCTGAAGGACAGCTCTTCAACCGACCTTACTGGAT
  ACAACGCGCCCAAGGAACAAACAATGCTATTTGCTGGAATAATAATCTTTTCCTGACCGTGGTCGATAAC
  ACCAGGAACACTAACTTTACAATTTCTGTTTCTAAAACCGCCATCGAAAATCCTAGTCAGTACAACTACA
  AAGCAGACAATTTTAAGCAATATTTGAGACACGTCGAAGAGTACGATGTGGAACTCGTGTTCCAGCTGTG
  TAAAGTCCCGCTGGAGCCGGACATCCTCGCTCATCTGAATGTCATGGACCCCACTATACTTGAAAACTGG
  CAACTGGCTTTTGTGCCACCCCCACCGCAGTCTATCGAGGATAATTACCGGTACATAAGGTCCTTGGCGA
  CAAGGTGCCCACCCGAGGAATCTGAAACTCAGTCAAAAGACCCCTATAAGGAATTCAACTTCTGGAACGT
  CGATCTCACCGAGCGGTTTAGTGCCGAGCTGAGCCAATTCCCCTTGGGACGGAAGTTTTTGTTCCAGACA
  GGACTGTTGAAAAGCTACACTCGCAGCGGAACGTCCAATAGTACAAAGCGCTTGCGCAGCATCTCTAGTA
  GTGGGACTACCCGGGCTAAACGCCGCAGAGTCCGCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101788 representing YP_002756551
  Red=Cloning sites Green=Tags
  
  MTLWLQQSGTIYLPPAKPVAKVLSTDEYVIGTNLFFYASSERLLTVGHPYFEIPNPALPGALAVPKVSGS
  QYRVFRCMLPDPNKFALIERSFYNPERERLVWKVRGLQLGRGGPLGLGTSGHPLFNKVADTENPTTYPAA
  ETDEQRVNLSMDPKQVQLLIVGCEPALGEHWDVAKPCSDEVNQNQGDCPPIQLLNTVIQDGDMCDIGFGA
  ANFKTLQQDKSGVPLDIVDSICKWPDLIKMEQNIYGDNLFFFTKKEQAYARHYFARAGTNGDPIPDGTTP
  NQDAFYLSPDQAGQAPQKTLASYTYFPTVSGSLVSSEGQLFNRPYWIQRAQGTNNAICWNNNLFLTVVDN
  TRNTNFTISVSKTAIENPSQYNYKADNFKQYLRHVEEYDVELVFQLCKVPLEPDILAHLNVMDPTILENW
  QLAFVPPPPQSIEDNYRYIRSLATRCPPEESETQSKDPYKEFNFWNVDLTERFSAELSQFPLGRKFLFQT
  GLLKSYTRSGTSNSTKRLRSISSSGTTRAKRRRVRQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012486
• ORF Size: 1578 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012486.1, YP_002756551
• RefSeq ORF: 1578
• Locus ID: 7701324
• MW: 59.4 kDa