E1 (NC_012486) Virus Tagged ORF Clone

CAT#: VC101784

Myc-DDK-tagged ORF clone of viral ORF for E1 protein [Human papillomavirus 112], codon optimized for human cell expression, YP_002756547

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101784 represents NCBI reference of YP_002756547 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAGCTTCCGAAGGGAACCGATGATTCAATCGAGAATGTTGAAACAGACTCTTCCTGGTTCGTGG
  TCACCGAAGCCGAATGTATAAACGACCTCGATAGTTTCGAGAACCTGTTTGAGGAAAGTACAGATGGAAG
  TCAGGTTAGTGATCTGATCGATGATGTCGACGACGCCTCACAAGGAAACAGCTTGGCCTTTTTCAACTCT
  CAGCTCGCCGAGGACTGTAATAAAGCCATCACTGACCTTAAGCGAAAATATTTGACTAGTCCCCAGCAGT
  GTGTGGCGGAGCTCTCCCCCCGGCTGGAGGCGGTCAAGATCACCGGAGAGGGCAAAAGTAAGAGGCGGCT
  GTTTCAAGACTCCGGCATTGAAGAGGATGAAATAGCATCCACCAGCCAGGTGTTGTCCGAGACGAATAAT
  AGCATTCCAGCCGATACCGACGAAACCCTCGACCTCCTGAAGTGCAATAACAGGAAGGCAACCTTTCTTT
  CAAAATTTAAAGAGGTATTCGCAGTGAGTTTCAATGAGCTGACTGGACTGTATAAAAACGATAAGACATG
  TTCCATTTGCTGGGTTGTAGCTGTATGTAACGCTAAGGAGGACTTGTTGAACAGTAGCAAGACCCTGCTG
  CAAACCCACGTGGACTTCGTGCAGATCATTACCGTCGGACTCTATGCACTCATGCTGATTGAATTCAAAA
  CGGGTAAAAGCCGAGAGACGGTAACCAATATGCTTTGTAAGCTGCTGAATATTAAGGACTATGAAGTGAT
  GTGCCAACCACCACGACTGAGAAGCGTGGCCGTCGCACTGTATTTTTACAAGAGGTCCCTGTCAAACGTT
  AGTTTCAAGCACGGTGAATTCCCCGAGTGGCTCGCCAAACAGATCCTGCTGGACCACAATGTCGGAAGTC
  AGGAAAGTTTTAGTCTCTCCGACATGGTGCAGTGGGCCTTTGACCATGAATACCTGGACGAATCTGAGAT
  TGCCTACAACTACGCCCTGGCAGCCACAGAAAACGCAAATGCTGCTGCCTTTCTCCAGTCTAACAATCAG
  GCTAAGCACGTAAAGGACTGTGCCATAATGGTGAAGCATTACAAGAGATATGAGATGCGACAGATGACAA
  TGGCCGCATGGATCAACAAATGTTGCCTGGAGTACGACGACACCGTGGACTGGAAGCCCATCGCCCACTT
  TTTGAAGTATCAGGGCATTAACTTCCTGGAATTCCTTATAGCATTTAAGCCTTTCTTGAAGGGCATTCCC
  AAGAAAAATTGCATGGTCTTCTGGGGGCCTCCTGATTCCGGTAAAAGTATGTTTTGTTTTAGCTTGCTGA
  AGTTCTTTAAGGGTAAGGTCATCAGTTACATGAACTCTCAGTCTCACTTCTGGTTGATGCCTTTGTCAGA
  TTGCAAGCTTGGACTTCTGGATGACGCTACGTACCCATGTTGGCAATACATGGATGTCCATCTGCGGAAC
  GCAATGGATGGCAACGCTATGTCAATAGATCTGAAACACAGGGCTCCCGTGCAGCTCAACCTGCCCCCTT
  TGCTTGTGACCACAAACACAGACGTGCTGAATGAACAGAGTCTGAAGTATCTGCACTCACGAATTAGATG
  TTTTAATTTCCCACACAAGGTACCGATTCTGGAGACCGGGGAGATCGCCTATAATATAACAAACATCAAT
  TGGGCCTGCTTTTTTCGGAAGTTCGCGAACCACCTCGAATTGCGGGAGGAGGATGGAGGCGATACCGGAA
  ATATTAACACAACCTTCCAATGTACAACACGCAGAAACTTTGAGCACTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101784 representing YP_002756547
  Red=Cloning sites Green=Tags
  
  MAELPKGTDDSIENVETDSSWFVVTEAECINDLDSFENLFEESTDGSQVSDLIDDVDDASQGNSLAFFNS
  QLAEDCNKAITDLKRKYLTSPQQCVAELSPRLEAVKITGEGKSKRRLFQDSGIEEDEIASTSQVLSETNN
  SIPADTDETLDLLKCNNRKATFLSKFKEVFAVSFNELTGLYKNDKTCSICWVVAVCNAKEDLLNSSKTLL
  QTHVDFVQIITVGLYALMLIEFKTGKSRETVTNMLCKLLNIKDYEVMCQPPRLRSVAVALYFYKRSLSNV
  SFKHGEFPEWLAKQILLDHNVGSQESFSLSDMVQWAFDHEYLDESEIAYNYALAATENANAAAFLQSNNQ
  AKHVKDCAIMVKHYKRYEMRQMTMAAWINKCCLEYDDTVDWKPIAHFLKYQGINFLEFLIAFKPFLKGIP
  KKNCMVFWGPPDSGKSMFCFSLLKFFKGKVISYMNSQSHFWLMPLSDCKLGLLDDATYPCWQYMDVHLRN
  AMDGNAMSIDLKHRAPVQLNLPPLLVTTNTDVLNEQSLKYLHSRIRCFNFPHKVPILETGEIAYNITNIN
  WACFFRKFANHLELREEDGGDTGNINTTFQCTTRRNFEHY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012486
• ORF Size: 1800 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012486.1, YP_002756547
• RefSeq ORF: 1800
• Locus ID: 7701320
• MW: 68.5 kDa