E7 (NC_014185) Virus Tagged ORF Clone

CAT#: VC101797

Myc-DDK-tagged ORF clone of viral ORF for transforming protein E7 [Human papillomavirus 121], codon optimized for human cell expression, YP_003668026

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101797 represents NCBI reference of YP_003668026 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCGGTCCAGAGAGCAAAGTAGGCGATATCGAGCTGCAGCTGGAGGAGCTGGTTATGCCAGCAGATC
  TGCTGTCAGATGAGGTTCTGAGCCCAGACGAGGACGAAGAGGAAGAGCTCGACGAACAACCTTACCGGAT
  CGATTCAGTCTGTCGCGTCTGTAAAACCAGTATTAGATTTTATGTGAGCGCTAGCGATCAGGGGATCCGG
  GGGCTTCAGCAGCTCCTCCTTTCACAGCTGTCCTTCATTTGCACTCGCTGTACACGCACTTCCTTCAGGC
  AGAACGGCAGGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101797 representing YP_003668026
  Red=Cloning sites Green=Tags
  
  MIGPESKVGDIELQLEELVMPADLLSDEVLSPDEDEEEELDEQPYRIDSVCRVCKTSIRFYVSASDQGIR
  GLQQLLLSQLSFICTRCTRTSFRQNGRQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014185
• ORF Size: 294 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_014185.1, YP_003668026
• RefSeq ORF: 294
• Locus ID: 9231099
• MW: 11.2 kDa