L2 (NC_014185) Virus Tagged ORF Clone

CAT#: VC101801

Myc-DDK-tagged ORF clone of viral ORF for minor capsid protein L2 [Human papillomavirus 121], codon optimized for human cell expression, YP_003668030

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101801 represents NCBI reference of YP_003668030 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATACTAGTAAAAGAGTTAAGCGCGATTCTGTCAAGCACCTGTACGAGCAGTGTAAGATTACAGGTA
  ATTGCCCGGACGACGTCAAAAATAAAGTCGAAGGAACCACCTTGGCGGACAGGCTGCTGCAATGGCTCGG
  ATCAGTTATCTACCTTGGAGGTCTTGGAATCGGAACTGGCAAGGGATCTGGCGGATCAACCGGCTATACA
  CCTCTCGGCCGGACACCCGGAGTTGGGTCCAGTGGGACCGTGGTACGCCCCACTATTCCCCTGGATCCGC
  TGGGGCCTACCGATATCATACCCATTGATACGGTGGATCCTGCAGGCCCTGCACTGGTGCCCCTCAATGA
  AGGCAACTTCCCAGATGTGACGGTGGTCGATGCTGGGGGAGCTACGGAGGACGTGACTGTAAGCGGCCCC
  GACCTCGGGTCAGGGGAAATCGCAATTATAACGGAGTCAGACCCAATTAGTGATGTCACAACCACTGGGG
  GGCACCCCACGGTTTCAACCACCGAGGACAATGTTGCTGTTATTGACGTCCAGACCGAGCCACCTCCTCC
  TAAGCGGCTCGCCTTCGAGAGAGGACCGCGGACATCCACCCCAACGCACATATCTGTTACACAGTCTATA
  GCAAACATAAGCGATCCCGGGAACATATTTGTAGATGCGCACTTCACTGGCCAGACAATCGGAGAAGAGA
  TTCCATTGACTAACCTGGGTTCTTTCGCTGAATTCGAGATCGAAGAGGCAGCTTCTGCTCCCAAACATTC
  AACCCCGAAAGCTTTCCAACGAGCCTATAGTAAGGCACGCGATTTGTACAACCGGAGGGTTCAGCAGGTT
  CAGACAAGAAATGTTCATTTTTTGAATCAGCCATCACGGCTGGTCCAATTCGAGTTTACAAACCCCGCCT
  TTGAGGAGGAGCAAATCTCCCTTCAGTTTCAACAGGACGTGCAACAGCTCGCAGCCGCACCCGACCCTGA
  CTTCCAAGACATTATCACACTCGGACGACCCCGGTTTTCCGAGACTGCGGATAATAGGATTCGGGTTAGT
  CGCCTGGGTCGACGGGGCACCATTCGCACCCGATCTGGCCTGCAGATTGGGCAGCAGGTGCACTTCTATC
  TCGATCTGTCCACTATTGACAATTCCGAGGCAATCGAATTGGGTGTGCTTGGGGAACATACCGGCGATGC
  TAGTATCATTAACCCCCAGGCCGAGTCCTCTTTTATTGATCTTCTGAGTGAGGAGCCAATCGCCCACACG
  GAGGATGAACTGTTGGACGATGTAGTGGAAGATTTCGGGAACGCGCATCTGATTCTGACGAGCAACACTC
  GCACACAGAGCATTACTATGCCGACCATTCCTCCGGGAGTCGCCCTGAAGGTATTCGTAACAGACATCGC
  CCAGCCCCTTTTTGTCTCATATCCACAAACCAACATTATTCCGGGCATACTGCCGACGACACCGACCTCC
  AACATCGAACCTGCAATCATTATCGACCTGGAGTCCGCTTCCGACTTCTTCATCCACCCAAGTCTCCTGA
  AGCGAAAAAGGAAGAGACCACTGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101801 representing YP_003668030
  Red=Cloning sites Green=Tags
  
  MNTSKRVKRDSVKHLYEQCKITGNCPDDVKNKVEGTTLADRLLQWLGSVIYLGGLGIGTGKGSGGSTGYT
  PLGRTPGVGSSGTVVRPTIPLDPLGPTDIIPIDTVDPAGPALVPLNEGNFPDVTVVDAGGATEDVTVSGP
  DLGSGEIAIITESDPISDVTTTGGHPTVSTTEDNVAVIDVQTEPPPPKRLAFERGPRTSTPTHISVTQSI
  ANISDPGNIFVDAHFTGQTIGEEIPLTNLGSFAEFEIEEAASAPKHSTPKAFQRAYSKARDLYNRRVQQV
  QTRNVHFLNQPSRLVQFEFTNPAFEEEQISLQFQQDVQQLAAAPDPDFQDIITLGRPRFSETADNRIRVS
  RLGRRGTIRTRSGLQIGQQVHFYLDLSTIDNSEAIELGVLGEHTGDASIINPQAESSFIDLLSEEPIAHT
  EDELLDDVVEDFGNAHLILTSNTRTQSITMPTIPPGVALKVFVTDIAQPLFVSYPQTNIIPGILPTTPTS
  NIEPAIIIDLESASDFFIHPSLLKRKRKRPLL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014185
• ORF Size: 1566 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_014185.1, YP_003668030
• RefSeq ORF: 1566
• Locus ID: 9231103
• MW: 56.4 kDa