L1 (NC_014185) Virus Tagged ORF Clone

CAT#: VC101802

Myc-DDK-tagged ORF clone of viral ORF for major capsid protein L1 [Human papillomavirus 121], codon optimized for human cell expression, YP_003668031

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101802 represents NCBI reference of YP_003668031 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTGCTGTGGATGCAAAATCCCGGTAAGCTGTATCTCCCACCAAGCCGCCCAGTCGCGAGGATCT
  TGAACACTGAGGAATATGTTAAGGGAACTAGTATATACTTTCACGCAGGCTCTGACAGGCTCCTTACTGT
  TGGCCACCCCTATTTTAACGTGTTCAATAACGGCGAAGATAAAGTGGTTGTGCCGAAGGTGAGCGGTAAT
  CAATATCGGGTCTTGCGAGTTATGCTCCCCGACCCAAACAAATTCGCTCTCGTGGACACAGACGTGTATA
  ATCCAGAAACTCAGCGGCTGGTCTGGAGACTGCGGGGAATAGAAATCCAGCGCGGTGGCCCTCTCGGGAT
  TGGGACCACCGGCCATCCACTGTTTAACAAGCTTGGCGACACCGAGAACCCTAACAAATACCTCACACAA
  AGTACAGATGACAGGCAGAATATGTCTCTTGACCCTAAACAAACCCAGCTGTTCATCGTAGGATGCAAAC
  CCTCTCTGGGTGCCCATTGGGATTTGGCCAAGCCATGTGTCACCGATCATCGCGAGAATGGGGACTGTTA
  TCCAATCGAGCTTGTGCACACTGTGATCGAGGATGGCGACATGGGGGACATTGGATTCGGGGCCGTCAAT
  TTCAAGAAATTCCAACAAGACAACAGCGGCGTTCCACTGGATATTGTGGCCTCCACTTGTAAGTGGCCGG
  ACTTCGTGAAGATGACGAAGGAATCTTACGGGGATAGTATGTTCTTCTACGGCAAACGGGAACAGCTGTA
  CGCACGGCATTACTACACACGGGCCGGAACAATGGGAGATGCCATCCCAGGACCATTCAACAACAAAACT
  GATTTTATCGTGCAACCCGACACAAAACAAAACCAGAAATCTATCTCATCACACATTTACTTTGGCACCC
  CATCCGGTAGTCTGGTGTCCAGTGAGGCATCTCTGTTTAACAGACCCTATTGGCTCCAAAGGGCTCAAGG
  CCAGAATAACGGGGTGCTCTGGAACAACCAACTTTTCATCACACTCTTCGACAACACACATAACACTAAT
  TTCACACTCAATTTTTATAAAAATGGAACGGATGTTCCTGAGCAGTACACTTACAAGGCCGAAGACTTTA
  AGCATTACCTTAGGCATGTTGAGGAGATAGAGTTCGAATTGATCCTTCAACTCTGTATTGTGCCGCTGGA
  ACCAGACGTTCTCTCACACATCAATGCAATGAATCCCTCCATCCTCGAAGACTGGAACCTGGCCTTTGTA
  CCCCAACCTACTAGTTCTATCGAGGATAAGTACCGCTTTTTGAGCTCTCTGGCTACACCATGCCCCGAAC
  AAGCAGTGACCTCCGAAAAGGTAGATCCCTATGCAAAGTATAACTTCTGGGTGGTTGACCTGAGCGAGAA
  GCTGAGTAGTGAACTCAGCCAGTTTTCATTGGGACGGCGATTCTTGTACCAGACAGGTCTGATGACAAAC
  GGAAACGGTGTGGTGAATCCAAGGAAACGAAGCCGGGTATCCCTCACAAAGAAGAGCGTTAAGAGGCGAA
  AGGTTGCCAAAGTTATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101802 representing YP_003668031
  Red=Cloning sites Green=Tags
  
  MALLWMQNPGKLYLPPSRPVARILNTEEYVKGTSIYFHAGSDRLLTVGHPYFNVFNNGEDKVVVPKVSGN
  QYRVLRVMLPDPNKFALVDTDVYNPETQRLVWRLRGIEIQRGGPLGIGTTGHPLFNKLGDTENPNKYLTQ
  STDDRQNMSLDPKQTQLFIVGCKPSLGAHWDLAKPCVTDHRENGDCYPIELVHTVIEDGDMGDIGFGAVN
  FKKFQQDNSGVPLDIVASTCKWPDFVKMTKESYGDSMFFYGKREQLYARHYYTRAGTMGDAIPGPFNNKT
  DFIVQPDTKQNQKSISSHIYFGTPSGSLVSSEASLFNRPYWLQRAQGQNNGVLWNNQLFITLFDNTHNTN
  FTLNFYKNGTDVPEQYTYKAEDFKHYLRHVEEIEFELILQLCIVPLEPDVLSHINAMNPSILEDWNLAFV
  PQPTSSIEDKYRFLSSLATPCPEQAVTSEKVDPYAKYNFWVVDLSEKLSSELSQFSLGRRFLYQTGLMTN
  GNGVVNPRKRSRVSLTKKSVKRRKVAKVI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014185
• ORF Size: 1557 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_014185.1, YP_003668031
• RefSeq ORF: 1557
• Locus ID: 9231104
• MW: 59.1 kDa