E4 (NC_014185) Virus Tagged ORF Clone

CAT#: VC101800

Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein, partial [Human papillomavirus 121], codon optimized for human cell expression, YP_003668029

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101800 represents NCBI reference of YP_003668029 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  AACATTAAGACCAAACTCTTTCTGCCGCTTCTGCCTCTGGCACCCCTGCCGCTGCCAGGCAATCTCCTCC
  GGGGGACCCCTGCTATCCCGCCTGGGACACCCAAGTTGAGTAGGCGCTACGACGAGTCTAGGCGCGGCCC
  TAGAATCCCCTTGTGCCCCCCTGTCCGCAAGCACCTTGATTTTGACATCACCGAGGACGACGAGAAGGAA
  AACCAGGAGCTGCCCGGAGCGCAGAGAGAAGAAGAGCCTACGTCCCAGACGCCCCCAGATCTGCTGACTC
  AGCTTTTGCACAAATGGGGCGTCGCCATCGATCAGTTGGCTGATATAATTCTGCAGGACCTGAAAGACTG
  TAAGCAAAAGCTGGGTATTCCTCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101800 representing YP_003668029
  Red=Cloning sites Green=Tags
  
  NIKTKLFLPLLPLAPLPLPGNLLRGTPAIPPGTPKLSRRYDESRRGPRIPLCPPVRKHLDFDITEDDEKE
  NQELPGAQREEEPTSQTPPDLLTQLLHKWGVAIDQLADIILQDLKDCKQKLGIPQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014185
• ORF Size: 375 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_014185.1, YP_003668029
• RefSeq ORF: 375
• Locus ID: 9231102
• MW: 14.0 kDa