E1 (NC_014185) Virus Tagged ORF Clone

CAT#: VC101798

Myc-DDK-tagged ORF clone of viral ORF for replication protein E1 [Human papillomavirus 121], codon optimized for human cell expression, YP_003668027

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101798 represents NCBI reference of YP_003668027 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGACAACAAGCCTGGAACTAATAATGCTGAGTCTCTTGCCGGCTGCTCTGACTGGTATATCGTGC
  GCGAGGCTGAGTGTAACGATAGTTTGGATACCCTTAACGACCTGGAGGAGCTTTTTGACAACAGCACAGG
  CTCCGATATTTCTAATCTGATCGATGATTTTGATGAAGTGGATCAAGGAAATTCTTTGGCTCTGTTTAAC
  GAGCAATGCACAGAGGATTGCAACAAAGCTATTGCCGAACTGAAGAGGAAATACGCGACCCCAAGCCCCA
  AAAAGTCATGCGCGGATATAGACCTCTCCCCCCGCCTGCAGACGGTCTCTCTGAGCTCTCAAAAAAATTC
  CAAGCGCCGGCTGTTCGAAAACTCCGGCAGCGCTGAGAACGAGGCTGAAGATTCCATTGAGGAGCAGGTA
  GAGACTGGAACGGAGAATAATAATGGGGCGGGGGACAATGGAGGCGACGCTATCGTGCAACTGCTTTCCA
  AAAGTAACTCAAAGGCAATTCTTCTGGCGAAGTTTAAGAGTGTATTTGGAGTGAGTTACAATGAGCTGAC
  TAGGCCTTTCAAATCAAACAAAACTATGTGTGATATTTGGGTTGTCATCGTGTATGGCGCCAACGATGAT
  ATGCTGGAGGCATCCAAGCAGATTCTGGAGCAGCAGTGTACTTATATTTTGCAGAAGAACTACGAGATTA
  TCTGCCTTTACCTCGTGGAGTTTAAGACCGGAAAATGTCGCGACACTGTTACCAAACTGTTCTGTTCTAT
  TCTGACCGTTAATGACATCCAAATTATGTGCGATCCTCCAAAACACCGGTCCGTCGCCACAGCATTGTAC
  TTCTATAAGTGCACAACTGGAAACGCTACTTATACGAAGGGTATAATGCCCGAGTGGATAACAAAGCAGC
  TCATGGTGGAGCATCAGTCTGCAACTGCATCTGAATCATTTGATTTCAGTGAGATGGTGCAGTGGGCCTA
  TGATCACGATTTCACCGAAGAGCCTGCCATAGCCTACGAGTACGCCTTGCTGGCACAAACTAATGCCAAT
  GCCGCTGCCTGGCTCAACCACAATAACCAGGTTAAGTTTGTGAGGGACTGCGCCTACATGTGCAAGCTCT
  ATAAGCGACAGGAAATGAAAGAAATGACGATGAGTAATTGGATTTGGAAGTGTTGTAGAAAACATGACGG
  GGAAGGTGACTGGAAGGTGATCCCGCAGTTCTTGAAATACCAGCAGGTGAATTTCATCGCATTTCTGAGT
  GCTTTGAAACCATTCCTCAAAGGCATCCCCAAGAAGAACTGTATACTCTTTTTTGGCAAGCCGAATACTG
  GCAAGAGCTACTTCGTTTACAGTCTTATTCGGTTCCTCGAGGGTAAAGTGGTGAGTTACATGAATTCTCA
  TTCTCATTTTTGGATACAACCACTGATTGATAGTAAAATTGGATTGATTGACGACGTCACCTACGCATGT
  TGGAGCTTCATTGATGTCCACATGAGGACCGGCCTGGATGGCAATCCTATCAGCATCGATTCAAAACACA
  AGGCCCCTCAACAGATGAGATTGCCCCCGCTGTTGGTGACCAGCAACATTGATCTCCACAAAGAGCAGTC
  CTTGGTCTACTTGCACTCTCGCGTCGTGGCTTTTGAATTCACCAAAGAAATGCCTTTCGATGACAATGGA
  GACCCTGTCTACAAGATTACCGATGTGTCTTGGAAGTATTTCTTTATAAAGCTGGCCAGACAACTGGAAC
  TGAGCCCTGAAGAGGAGCAGGACGGTGAAGCCGAACGGGCATTGCGATGCTGCGCTCGGAGCTCCATCGA
  TAGCATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101798 representing YP_003668027
  Red=Cloning sites Green=Tags
  
  MGDNKPGTNNAESLAGCSDWYIVREAECNDSLDTLNDLEELFDNSTGSDISNLIDDFDEVDQGNSLALFN
  EQCTEDCNKAIAELKRKYATPSPKKSCADIDLSPRLQTVSLSSQKNSKRRLFENSGSAENEAEDSIEEQV
  ETGTENNNGAGDNGGDAIVQLLSKSNSKAILLAKFKSVFGVSYNELTRPFKSNKTMCDIWVVIVYGANDD
  MLEASKQILEQQCTYILQKNYEIICLYLVEFKTGKCRDTVTKLFCSILTVNDIQIMCDPPKHRSVATALY
  FYKCTTGNATYTKGIMPEWITKQLMVEHQSATASESFDFSEMVQWAYDHDFTEEPAIAYEYALLAQTNAN
  AAAWLNHNNQVKFVRDCAYMCKLYKRQEMKEMTMSNWIWKCCRKHDGEGDWKVIPQFLKYQQVNFIAFLS
  ALKPFLKGIPKKNCILFFGKPNTGKSYFVYSLIRFLEGKVVSYMNSHSHFWIQPLIDSKIGLIDDVTYAC
  WSFIDVHMRTGLDGNPISIDSKHKAPQQMRLPPLLVTSNIDLHKEQSLVYLHSRVVAFEFTKEMPFDDNG
  DPVYKITDVSWKYFFIKLARQLELSPEEEQDGEAERALRCCARSSIDSI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014185
• ORF Size: 1827 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_014185.1, YP_003668027
• RefSeq ORF: 1827
• Locus ID: 9231100
• MW: 69.3 kDa