E6 (NC_005134) Virus Tagged ORF Clone

CAT#: VC102035

Myc-DDK-tagged ORF clone of viral ORF for E6 [Human papillomavirus type 96], codon optimized for human cell expression, NP_932319

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102035 represents NCBI reference of NP_932319 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGTATCTGATTCTGGCAGTGACCCCTGGCACCACACCGATACAGTGGCTGTGTCAGAAGCAGTTCA
  AGCCGGCCAAGAAATCCCCCCCCAAAAGGCTGCCCACCCTGGAACCCCTTACCGTGTCATTCGCTACCCT
  TTCAGTTGCTAAATCCTTCATCCTGAGAGGATGCTGGCAACTCCAGCCCGCAACGCACAAGCAGGTTACA
  GCCTGCGTCTCACGAACGTTCTATATCCAAACATACAGAAGCCTGTTCATCGTCGAGATGGACTTGAAAA
  CCGTCCAACAGCTGCGGAGTCATCTGGGCATCCCAACTGAGGACCTGCTCCTTGCTTGCAATTTCTGTAA
  GAAGTTTTTGACCTTTGAGGAGCTTCTGGGCTTTGATTCCAAGACTCTTAACCTTATCTGGAAAGAAGGT
  TATGCCTACGCTTGCTGTTTTAACTGCGCCAGAACTGTCGCTAAAGTAGAGTTTGAACACTTTTACGAGA
  AAACAGTGAAGGGCCGGGAAATAGAGAGCAGGTCCGGTTCACTCCTGTGTTGCATAGTAGTGAGATGCCA
  GTTCTGTCTCAGACTCCTCGACTACCTGGAAAAGCTGAATATTTGTGGTTACCAGGAGAATTTTTACCTG
  GTGAGGGGGGGCTGGAAAGGAATTTGCCGCTACTGCCGGGAGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102035 representing NP_932319
  Red=Cloning sites Green=Tags
  
  MQYLILAVTPGTTPIQWLCQKQFKPAKKSPPKRLPTLEPLTVSFATLSVAKSFILRGCWQLQPATHKQVT
  ACVSRTFYIQTYRSLFIVEMDLKTVQQLRSHLGIPTEDLLLACNFCKKFLTFEELLGFDSKTLNLIWKEG
  YAYACCFNCARTVAKVEFEHFYEKTVKGREIESRSGSLLCCIVVRCQFCLRLLDYLEKLNICGYQENFYL
  VRGGWKGICRYCREI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_005134
• ORF Size: 675 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_005134.2, NP_932319
• RefSeq ORF: 675
• Locus ID: 2943332
• MW: 26.0 kDa