E2 (NC_005134) Virus Tagged ORF Clone

CAT#: VC102037

Myc-DDK-tagged ORF clone of viral ORF for E2 [Human papillomavirus type 96], codon optimized for human cell expression, NP_932322

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102037 represents NCBI reference of NP_932322 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCCTTAATCAGCGCTTCAATGCTTTGGAGGACCAGCTGATGGATATATATGAACAGGGTTCTG
  ATAAGCTGGAAGATCAAATTAAGCACTGGAACTTCCTGAGAAAGGAACAGATTCTGCTCCACTACGCACG
  ACGCAGGGGAATCATGCGCCTCGGGTACACGCCGGTGCCTTCTCTGGCGGCCAGTGAAACCAAAGCCAAA
  GACGTAATCTACATGGTGCTGCAGCTTGAAAAGCTGCGAGATTCACCTTACAAGGATGAGCCCTGGACAC
  TCATAAACACTAGCCTGGAGACCTACAGGGCACCTCCGGTGAACTGTTTCAAAAAAGGTCCGCAGAACGT
  TGAGGTCATTTTCGATGCCGATCCGGAGAACATTATGTTGTACACCTCTTGGCAGTTTATTTACTTTGAG
  GATACCGACGGGCACTGGCAGAAAACCGAAGGTAGGATTGACTATGCAGGGCTCTATTACCTGGAAGGCC
  AGCTGAGGCACTACTATGTCGAATTTAAGGTGGATGCCCGCCGGTTTGGAACATCCGGCGAATGGGAGGT
  ACGGTTCAACGGGGAAACCATCTTTGCCCCAGTGACATCATCTTCTCCATCAAGTTATGAGGAGGTCAGA
  GAACGGGCAGAACCCCCGGACGTGCCTGAGGTCGGGACTGCCCCCGATTCTGGCACTCTCCAGACAGGTC
  GGCCCGGCACTGAAACAGCAAAGGGACGACGGTATGGGAGGAAGGAGAGCTCACCCACTGTGGCATCCTT
  GAGAGTCAGGCAAAAGGTGAGCCGATCCACAACCAGAAAGGCTGAGGAGCGACGCTCCAGATCCCCCAGC
  TCAGGGACCGAACAGGCTGGCGCCCGCGGACGCGGCCAAAGAGGAACAAGAGCCAGGAGTAGAACTAGGA
  GCAGATCAAGGCGGCGATCCCGCAGTAGTTCCAGAGCCCGATCACGGCGCGCGCGAGAACATAGCGAAAG
  CAGGGGTGGACGGGGACGGGGAGCATACGCTACCAGGAGTAGATCTAGGTCTGCAGAATGCAGGTCAATA
  GACCAGTGTGGCATCCCCGCTTCACAAGTTGGCCGATCAGTACAGTCCGTGGGCCGCAATAATTCTAGTC
  GCCTGAAGAGACTCCTGGACGAGGCCCTCGACCCCCCTGTGATACTGCTCCGCGGCGAACCCAATACGCT
  GAAATGTTACCGATATCGCGTGAAAGATAAATTGCGCGGCAGTTTCGACAAGATATCTACTACGTGGTCT
  TGGGTTGCCAGCCAGGGCAATACGCGGATCGGGAGAGCAAGGATGCTGCTGTCATTCGTCAGTGAGGATC
  AACGCGAAAAATTTATTAACGTGATGAAGCTGCCTAAAGGGGTGGACTGGAGTTTCGGCAGCTTCGACAG
  TCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102037 representing NP_932322
  Red=Cloning sites Green=Tags
  
  MEALNQRFNALEDQLMDIYEQGSDKLEDQIKHWNFLRKEQILLHYARRRGIMRLGYTPVPSLAASETKAK
  DVIYMVLQLEKLRDSPYKDEPWTLINTSLETYRAPPVNCFKKGPQNVEVIFDADPENIMLYTSWQFIYFE
  DTDGHWQKTEGRIDYAGLYYLEGQLRHYYVEFKVDARRFGTSGEWEVRFNGETIFAPVTSSSPSSYEEVR
  ERAEPPDVPEVGTAPDSGTLQTGRPGTETAKGRRYGRKESSPTVASLRVRQKVSRSTTRKAEERRSRSPS
  SGTEQAGARGRGQRGTRARSRTRSRSRRRSRSSSRARSRRAREHSESRGGRGRGAYATRSRSRSAECRSI
  DQCGIPASQVGRSVQSVGRNNSSRLKRLLDEALDPPVILLRGEPNTLKCYRYRVKDKLRGSFDKISTTWS
  WVASQGNTRIGRARMLLSFVSEDQREKFINVMKLPKGVDWSFGSFDSL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_005134
• ORF Size: 1404 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_005134.2, NP_932322
• RefSeq ORF: 1404
• Locus ID: 2943331
• MW: 53.6 kDa