E1 (NC_005134) Virus Tagged ORF Clone

CAT#: VC102041

Myc-DDK-tagged ORF clone of viral ORF for E1 [Human papillomavirus type 96], codon optimized for human cell expression, NP_932321

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102041 represents NCBI reference of NP_932321 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGACAATAGAGGCATCGATCCTAAGGAGGGGTGTAGCAATTGGTTCAGCCTGGAAGCCGACTGCA
  GCGATCTCGACGATGACCTTGAGAAACTGTTTGAGGAGGGCACTAACAGCGACCTCTCAGACCTGATCGA
  CGACGGAGATGCCGTGGATAATGCAAACGAACAGGGTATTAGCAGAGACCTCTTCCGACAGCAAGGCAGC
  GAGGAATTCGAACAACAAATCCAAGACCTTAAGCGCAAATATTTTAGCCCAAAGGAGCTGCAGCAGCTTT
  CACCTAGACTTGAAGCCATTCCACTGAGCCCACAGCATAAGTGTAAAAAGCGCCTGTTCATGGAGCAGGA
  TAGCGGTCTGGAACTGAGCCTGACTCACGAATGTGAAGATACTGCACCTGAAGTTGAAGTGCCCCCCAAT
  ACCGACGGCACTGCCCGGACGGAAGTCCAGGCCAGTAACGAACACTATCGACTGCTGCTGAAATGTTCCA
  ATTTGAGAGCTACTATGCTGTCCAAGTTCAAGAATTCATTCGGGGTGGGCTTCATGGAACTGTGTAGGAA
  GTTTAATTCAAATAAGACCTGCTGCAGGGACTGGGTAGTAACAGCTTACGGGGTGAAGGAAGAGCTGCTG
  GAAGGATGCAAGCAGCTTCTCCAGGAGCACTGTGGATACATTTGGTTGCACACGCTGTGTCCCATGAGCC
  TTTTTCTTCTCTGTTTCAAAACTGGTAAGAGCCGGGACACCGTGGTAAGACTGTTGCAGCGCATGTTGTC
  AATCCACAAGGAGCAGCTGCTCACCGAGCCTCCCAAGCTCAGGAGCGTTATGGCCGTTTTGTACTGGTAT
  AAAGGCTCCATGAATCCGAATATCTACGCATTTGGGGAGTATCCCGACTGGATAGTCCAGCAGACAATGA
  TCAGCCACCACGAGGGCGATAATTTGCAGTTTGAGCTCTCACCTATGGTCCAGTGGGCTTATGATAACGA
  TTACATTGAGGATTCCGATATCGCCTATAACTACGCCAAGCTGGCGGACGAGGACATCAACGCGCGGGCG
  TTCCTTGCTCACAATAACCAGGCTAAAATCGTTCGAGACTGTGCGTGGATGGTCAGGCACTATAAACGGG
  GGGAAATGCGCTACATGTCAATCTCTAAATGGATATGGTATAAGTTGAAAAAAGTGGAAAACGGAGGACA
  CTGGAGCAACATTGTCAAGTTTGTACGCTTTCAGGGTATAAACTTTATTATGTTCCTGGATGCATTTAAG
  CACTTTCTGCTGTCCCGCACTAAGAAGAACTGTATCCTTTTTTACGGACCCAGCGACTGCGGAAAAACCA
  TGTTTTGTATGAGCCTCATTAAGGCACTGGGCGGCAGAGTTATTTCTTTCGCCAACGCCAAATCTCAGTT
  CTGGCTGCAGCCTCTCACAGAATCTAAGATAGCGATGCTGGACGACGCCACAGAAGCATGCTGGAACTAC
  CTCGATACTTACTTGCGCAACGGGCTGGACGGGAATTGGGTTAGCGTGGACTGCAAGCACAAAGCACCCA
  TTCAGATTAGGTTCCCGCCCTTGCTGATAACCAGTAACTACGATATCTTGAAAAATGAGAGGTACAGGTT
  CCTGGTTTCACGCATCAAGATTTTCGAGTTCAAGAACAAATTCCCATTCAATGAGGACGGGACCCCAATG
  TTTGAGCTCACCGACCAAAGCTGGAAGAGCTTTTTCCAGAGGCTGTGGAAGCAGTTGGACCTTAGTGACC
  AAGAGGACGAAGGTGAAGACGGAGGATCCGAGCCCGCATTTCAATGCTCACGCCGCCCGGCCCATGGGCA
  TATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102041 representing NP_932321
  Red=Cloning sites Green=Tags
  
  MADNRGIDPKEGCSNWFSLEADCSDLDDDLEKLFEEGTNSDLSDLIDDGDAVDNANEQGISRDLFRQQGS
  EEFEQQIQDLKRKYFSPKELQQLSPRLEAIPLSPQHKCKKRLFMEQDSGLELSLTHECEDTAPEVEVPPN
  TDGTARTEVQASNEHYRLLLKCSNLRATMLSKFKNSFGVGFMELCRKFNSNKTCCRDWVVTAYGVKEELL
  EGCKQLLQEHCGYIWLHTLCPMSLFLLCFKTGKSRDTVVRLLQRMLSIHKEQLLTEPPKLRSVMAVLYWY
  KGSMNPNIYAFGEYPDWIVQQTMISHHEGDNLQFELSPMVQWAYDNDYIEDSDIAYNYAKLADEDINARA
  FLAHNNQAKIVRDCAWMVRHYKRGEMRYMSISKWIWYKLKKVENGGHWSNIVKFVRFQGINFIMFLDAFK
  HFLLSRTKKNCILFYGPSDCGKTMFCMSLIKALGGRVISFANAKSQFWLQPLTESKIAMLDDATEACWNY
  LDTYLRNGLDGNWVSVDCKHKAPIQIRFPPLLITSNYDILKNERYRFLVSRIKIFEFKNKFPFNEDGTPM
  FELTDQSWKSFFQRLWKQLDLSDQEDEGEDGGSEPAFQCSRRPAHGHI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_005134
• ORF Size: 1824 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_005134.2, NP_932321
• RefSeq ORF: 1824
• Locus ID: 2943334
• MW: 70.7 kDa