L2 (NC_005134) Virus Tagged ORF Clone

CAT#: VC102039

Myc-DDK-tagged ORF clone of viral ORF for L2 [Human papillomavirus type 96], codon optimized for human cell expression, NP_932324

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102039 represents NCBI reference of NP_932324 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCGGGCTAGACGGGTGAAGCGCGATTCCGTCACAAATATCTATAGGGGCTGTAAGGCTGCGGGGA
  CATGTCCTCCCGACGTGATCAACAAAGTGGAGCAAAAGACTATCGCAGATCAGATCCTGAAGTATGGATC
  AACTGCCGCCTTCTTCGGGGGCCTTGGGATCAGCACAGGAAAAGGAACCGGAGGATCAACCGGATACGTC
  CCTCTCCCAGAAGGCCCAGCGCCCGGTGTGCGGGTAGGCGGTACTCCAACCGTGGTCCGACCAGGCGTAA
  TCCCCGAAGCAATCGGACCCACAGACATCATACCTCTCGATACAGTAAACCCAATTGACCCTGTCGCCCC
  CAGTGTTGTACCCCTCACCGATACCGGACCCGACCTGTTGCCTGGGGAGATTGAGACAATCGCTGAAGTG
  CACCCGGTTAGTGACGTCACTCCCGTTGACACTCCAGTGGTAACCGGCGGCCGGGGCAGCTCAGCTGTGT
  TGGAGGTTGCAGACCCAAGTCCCCCGACGAGGGCACGGGTCTCACGGACCCAATACCACAATCCAGCCTT
  TCAGATCATAAGCGAGACAACACCCACTACCGGCGAGGCATCCCTTAGTGACCAAATTATCGTGCAGTCC
  GGCAGCGGCGGGCAGAATATTGGTGGCAGTGGCCCGTCTGTCGAAATTGAGCTGGAGGAGTTTCCTACCC
  GATATTCCTTCGAGATCGAGGAGCCAACCCCCCCGAGAAAGACTTCAACCCCCGTTAGAATGGCTCAGCA
  GGCTTCAAGAGCTCTGAGGCGGGCACTCTATAATCGAAGGCTTACACAACAGGTGAGCGTTGAAAATCCC
  CTCTTTCTTCAGCAGCCCTCCAAGCTGGTTACCTTCCAGTTCGATAACCCGGCGTACGAGGAGGAAATCA
  CGCAGATTTTTGAACGAGACCTGTCCAGTATCGAGGAGCCCCCCGATCGACAATTCATGGACGTCGTGAA
  ACTGGGAAGGCCGACTTACGCCGAGACCCCCGAAGGGTATATCCGCGTGTCCCGCCTGGGAAAGAGGGCC
  ACCATCCGCACACGCAGCGGAGCGCAAGTGGGAACTCAGGTTCACTTCTACCGCGACATAAGCACTATCG
  ACACCGAACCATCAATCGAACTTCAGCTGTTGGGAGAGCATTCTGGCGACGCTAGCATCGTGCAGGGTCC
  AGTAGAGTCAACGTTTGTTAATATGGACGTGCAAGAGATACCAACCCTTGAGGAGGTGCCCGAACTGCAC
  AGTGAAGATGTGCTCCTGGAAGAAGCCTTGGAGGATTTTTCCGGAGCTCAACTGGTCTTTGGCAATTCCA
  GACGGTCCAACGTTATCACAATCCCGAGGTTCGAAACGCCAAGAGAAATTAACATTTATACCCCAGATCT
  GGACGGATACTATATTTCCTACCCTGAAACACGCAATATTCCCGAAGTCATTTACACTGAGCCAGACACG
  ACACCTACCATAATCATCCACACGGAAGACTTCAGCGGCGATTATTACCTGCACCCTTCACTCCGCCGCC
  GGAAACGCAAGAGAGCCTACTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102039 representing NP_932324
  Red=Cloning sites Green=Tags
  
  MARARRVKRDSVTNIYRGCKAAGTCPPDVINKVEQKTIADQILKYGSTAAFFGGLGISTGKGTGGSTGYV
  PLPEGPAPGVRVGGTPTVVRPGVIPEAIGPTDIIPLDTVNPIDPVAPSVVPLTDTGPDLLPGEIETIAEV
  HPVSDVTPVDTPVVTGGRGSSAVLEVADPSPPTRARVSRTQYHNPAFQIISETTPTTGEASLSDQIIVQS
  GSGGQNIGGSGPSVEIELEEFPTRYSFEIEEPTPPRKTSTPVRMAQQASRALRRALYNRRLTQQVSVENP
  LFLQQPSKLVTFQFDNPAYEEEITQIFERDLSSIEEPPDRQFMDVVKLGRPTYAETPEGYIRVSRLGKRA
  TIRTRSGAQVGTQVHFYRDISTIDTEPSIELQLLGEHSGDASIVQGPVESTFVNMDVQEIPTLEEVPELH
  SEDVLLEEALEDFSGAQLVFGNSRRSNVITIPRFETPREINIYTPDLDGYYISYPETRNIPEVIYTEPDT
  TPTIIIHTEDFSGDYYLHPSLRRRKRKRAYL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_005134
• ORF Size: 1563 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_005134.2, NP_932324
• RefSeq ORF: 1563
• Locus ID: 2943336
• MW: 57.1 kDa