E4 (NC_005134) Virus Tagged ORF Clone

CAT#: VC102038

Myc-DDK-tagged ORF clone of viral ORF for E4 [Human papillomavirus type 96], codon optimized for human cell expression, NP_932323

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102038 represents NCBI reference of NP_932323 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGCTGACCTCGAATTGGTTGAAAACGGCAAGTGTGTCTTGACAGGCAAACTGTCTCTGCTGCTGC
  TCCCTGCACCACGACGACCTCATACTAAACGGTCCGAAAATGGACTCAGCCCTCCAACTTCTCCGAGGTC
  CGAGCCTCCACTTACTCCGGCCCCGTATCGCTTGGGGGATCTGGAGCCAAAACCCCCCAAAGGGGAGGGG
  ACGGACGAGAAAAATTTGGTCCTGCAACTGCCTCCATCAGGATCTGGGAAGAAATATCACGATTTGCTCC
  ACGGTAAGCCAAAGAACGATGGACCTGGCGTGCACAGGCAGGGGCAGTCCAAGCCCGTCCATGGCGATGG
  GGATAACGGGGAACCAGGTCCGGTCCCAGAACCTGAGGCGGGTGCTGGCGCAGGAGCAGGTGCCGCAGCT
  GAACCTGAGGCCGGAGGCCAGGGAAACACACAGAACCGAGGGGAGGGGGAGGTTGAAGGCAGGACCCCGC
  AGGACCCTGAGGTAGACCTCCAAAGCGCCGATCTGCTGACCAGCGTAGCATTTCAGCTCCATAAGTGGGA
  GGGACAGTTTAACCAGCTGGTAGAGACTATACTCCGCGACCTGAGGGATTACTGGATGAAGTTGTCAACC
  CCCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102038 representing NP_932323
  Red=Cloning sites Green=Tags
  
  MLADLELVENGKCVLTGKLSLLLLPAPRRPHTKRSENGLSPPTSPRSEPPLTPAPYRLGDLEPKPPKGEG
  TDEKNLVLQLPPSGSGKKYHDLLHGKPKNDGPGVHRQGQSKPVHGDGDNGEPGPVPEPEAGAGAGAGAAA
  EPEAGGQGNTQNRGEGEVEGRTPQDPEVDLQSADLLTSVAFQLHKWEGQFNQLVETILRDLRDYWMKLST
  PQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_005134
• ORF Size: 636 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_005134.2, NP_932323
• RefSeq ORF: 636
• Locus ID: 2943335
• MW: 22.6 kDa