NS (NC_000883) Virus Tagged ORF Clone

CAT#: VC102080

Myc-DDK-tagged ORF clone of viral ORF for non-structural protein NS1 [Human parvovirus B19], codon optimized for human cell expression, YP_004928144

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102080 represents NCBI reference of YP_004928144 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACTTTTCCGGGGAGTTCTCCAAGTTAGTTCCAATGTATTGGACTGCGCCAACGACAACTGGTGGT
  GTAGCCTTTTGGACCTGGACACATCAGATTGGGAGCCCCTGACACATACAAATCGGTTGATGGCTATTTA
  CCTGTCCTCAGTAGCAAGCAAGCTGGACTTGACTGGCGGCCCTCTGGCAGGCTGCCTGTACTTCTTTCAG
  GCCGAGTGTAATAAGTTTGAAGAGGGTTATCACATTCACGTGGTCATTGGTGGGCCTGGTCTTAACCCGC
  GCAATCTTACGGTGTGTGTTGAAGGGCTCTTCAATAACGTATTGTATCACTTCGTTACTGAAAATGTCAA
  ATTGAAATTCCTGCCTGGCATGACCACCAAAGGGAAATACTTTCGGGATGGCGAGCAGTTTATCGAGAAC
  TACCTGATGAAAAAGATTCCACTGAACGTGGTATGGTGCGTGACAAATATCGACGGCTATATCGACACAT
  GTATATCAGCCACCTTCCGCAGAGGGGCTTGTCACGCTAAGAAGCCCCGGATCACCACCGCCATAAACGA
  CACTAGCTCCGATGCCGGCGAATCCTCCGGTACCGGAGCCGAGGTCGTGCCGTTTAATGGGAAAGGCACT
  AAAGCCAGCATCAAATTTCAGACTATGGTTAATTGGCTTTGCGAGAACAGAGTTTTTACTGAAGATAAAT
  GGAAGCTGGTCGACTTCAACCAGTACACATTGCTCAGCTCATCACATTCAGGATCCTTCCAAATACAATC
  AGCGCTGAAGCTGGCAATTTACAAGGCGACCAATCTCGTGCCGACAAGCACTTTCCTGTTGCACACAGAC
  TTCGAGCAGGTAATGTGTATAAAGGATAACAAGATCGTCAAATTGCTTCTGTGCCAGAACTATGATCCCT
  TGCTGGTGGGCCAACATGTGCTGAAGTGGATAGACAAGAAGTGCGGTAAAAAAAACACATTGTGGTTCTA
  CGGCCCCCCGAGCACTGGTAAGACTAACCTCGCAATGGCAATCGCCAAAAGTGTGCCTGTTTATGGAATG
  GTCAATTGGAACAACGAGAACTTTCCATTCAATGACGTGGCCGGCAAGTCCCTTGTGGTGTGGGACGAGG
  GGATCATCAAGAGTACCATTGTGGAAGCCGCAAAAGCCATACTGGGCGGCCAGCCTACGCGCGTGGATCA
  GAAAATGCGCGGCAGCGTCGCCGTCCCGGGAGTCCCCGTTGTAATTACAAGTAATGGAGATATTACTTTC
  GTTGTGAGCGGGAATACCACAACAACGGTTCACGCCAAGGCGCTTAAGGAGCGAATGGTGAAACTTAACT
  TCACAGTGCGGTGTTCCCCCGACATGGGGCTTCTGACAGAGGCAGACGTTCAGCAATGGTTGACATGGTG
  TAATGCTCAGTCCTGGGATCACTATGAGAATTGGGCTATCAACTACACCTTTGATTTCCCAGGGATTAAT
  GCTGATGCCCTTCATCCGGACCTCCAGACTACGCCTATTGTGACAGACACCAGCATCAGCAGCAGTGGCG
  GGGAAAGTAGCGAAGAACTTTCTGAGAGCTCTTTTTTCAACCTCATAACCCCGGGGGCATGGAACACGGA
  GACTCCCAGGTCTAGCACTCCTATCCCAGGTACGTCTTCCGGCGAAAGCTTTGTCGGCTCCCCGGTCAGC
  AGCGAGGTGGTCGCCGCTTCATGGGAAGAAGCCTTCTACACGCCTCTGGCCGATCAGTTCAGGGAACTGC
  TGGTCGGTGTAGATTACGTCTGGGATGGGGTGAGGGGCCTGCCTGTCTGTTGTGTGCAGCACATCAATAA
  CTCCGGCGGAGGGCTGGGTCTGTGCCCACACTGTATCAATGTAGGAGCGTGGTATAACGGATGGAAGTTT
  AGGGAATTTACCCCGGACCTGGTGCGATGCAGCTGCCACGTCGGGGCGTCCAATCCGTTTAGTGTATTGA
  CCTGTAAGAAGTGCGCTTACTTGTCAGGCCTGCAATCCTTTGTGGACTATGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102080 representing YP_004928144
  Red=Cloning sites Green=Tags
  
  MELFRGVLQVSSNVLDCANDNWWCSLLDLDTSDWEPLTHTNRLMAIYLSSVASKLDLTGGPLAGCLYFFQ
  AECNKFEEGYHIHVVIGGPGLNPRNLTVCVEGLFNNVLYHFVTENVKLKFLPGMTTKGKYFRDGEQFIEN
  YLMKKIPLNVVWCVTNIDGYIDTCISATFRRGACHAKKPRITTAINDTSSDAGESSGTGAEVVPFNGKGT
  KASIKFQTMVNWLCENRVFTEDKWKLVDFNQYTLLSSSHSGSFQIQSALKLAIYKATNLVPTSTFLLHTD
  FEQVMCIKDNKIVKLLLCQNYDPLLVGQHVLKWIDKKCGKKNTLWFYGPPSTGKTNLAMAIAKSVPVYGM
  VNWNNENFPFNDVAGKSLVVWDEGIIKSTIVEAAKAILGGQPTRVDQKMRGSVAVPGVPVVITSNGDITF
  VVSGNTTTTVHAKALKERMVKLNFTVRCSPDMGLLTEADVQQWLTWCNAQSWDHYENWAINYTFDFPGIN
  ADALHPDLQTTPIVTDTSISSSGGESSEELSESSFFNLITPGAWNTETPRSSTPIPGTSSGESFVGSPVS
  SEVVAASWEEAFYTPLADQFRELLVGVDYVWDGVRGLPVCCVQHINNSGGGLGLCPHCINVGAWYNGWKF
  REFTPDLVRCSCHVGASNPFSVLTCKKCAYLSGLQSFVDYE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000883
• ORF Size: 2013 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000883.2, YP_004928144
• RefSeq ORF: 2013
• Locus ID: 1489597
• MW: 74.1 kDa