VP1 (NC_000883) Virus Tagged ORF Clone

CAT#: VC102081

Myc-DDK-tagged ORF clone of viral ORF for capsid protein 1 [Human parvovirus B19], codon optimized for human cell expression, YP_004928146

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102081 represents NCBI reference of YP_004928146 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAAAAGAAAGCGGCAAATGGTGGGAGTCTGATGATGAGTTTGCTAAGGCTGTGTATCAGCAGTTTG
  TGGAGTTCTATGAGAAAGTTACTGGGACTGATCTCGAGTTGATTCAGATCCTGAAGGACCACTACAACAT
  ATCCCTGGATAATCCACTGGAAAATCCCTCCTCACTGTTTGACTTGGTCGCTCGCATTAAGAATAACTTG
  AAGAATAGTCCAGATCTGTACTCCCATCATTTCCAGAGCCACGGACAGCTGTCCGATCACCCTCACGCGC
  TGTCAAGTTCCTCAAGTCACGCAGAACCTCGCGGAGAGGACGCAGTGCTCTCTTCTGAGGACCTCCATAA
  ACCCGGGCAAGTCTCAGTTCAACTCCCCGGGACCAACTACGTGGGACCTGGCAACGAACTCCAAGCGGGA
  CCTCCCCAGAGCGCCGTCGACAGCGCCGCAAGAATTCATGACTTCCGCTACAGTCAGCTTGCTAAACTGG
  GGATCAACCCCTACACCCACTGGACTGTGGCCGACGAGGAACTCCTGAAAAACATTAAAAATGAGACCGG
  ATTTCAAGCCCAAGTCGTGAAGGACTACTTCACACTGAAAGGGGCTGCTGCTCCAGTGGCCCATTTCCAA
  GGAAGCCTGCCAGAGGTTCCAGCATATAATGCTTCTGAGAAATACCCCTCTATGACATCAGTGAATTCTG
  CTGAAGCGAGTACAGGCGCAGGAGGAGGTGGTAGTAACCCTGTCAAAAGTATGTGGTCTGAGGGAGCCAC
  TTTCAGTGCAAACTCAGTTACCTGTACGTTTTCAAGGCAGTTTCTGATCCCATATGATCCTGAACACCAT
  TACAAGGTATTTTCACCAGCCGCATCTTCATGTCACAATGCATCAGGCAAGGAAGCTAAGGTGTGCACCA
  TCTCACCCATCATGGGATACTCTACACCCTGGCGGTACCTGGATTTTAATGCACTGAACCTGTTCTTCTC
  CCCCCTGGAATTTCAGCATTTGATCGAGAACTACGGTTCAATAGCCCCCGATGCCCTGACCGTGACTATT
  AGTGAGATCGCCGTCAAGGATGTGACCGACAAGACTGGTGGTGGAGTGCAAGTGACCGACTCTACCACAG
  GCCGGCTGTGTATGCTGGTGGACCACGAATACAAATATCCCTATGTCCTGGGCCAAGGCCAGGATACGCT
  TGCTCCCGAATTGCCGATTTGGGTTTACTTCCCGCCTCAGTACGCTTACTTGACCGTCGGTGACGTGAAT
  ACCCAGGGCATTTCCGGAGACTCCAAGAAGCTGGCTTCTGAGGAGTCAGCTTTTTATGTGCTGGAGCACT
  CCTCTTTCCAGCTTTTGGGAACCGGTGGGACAGCTACCATGTCCTACAAATTTCCGCCCGTACCACCAGA
  AAATCTGGAAGGATGTAGTCAGCATTTCTATGAGATGTATAACCCCCTTTACGGCTCTCGCTTGGGGGTG
  CCGGACACACTGGGCGGCGACCCTAAATTCAGATCACTGACTCACGAGGATCATGCGATCCAACCTCAGA
  ACTTCATGCCCGGCCCCCTCGTGAACAGCGTGTCTACCAAGGAAGGCGACTCCTCTAACACCGGAGCAGG
  CAAGGCACTGACCGGGCTCAGCACAGGAACAAGCCAGAACACCAGGATCAGCCTCCGGCCCGGCCCAGTG
  AGCCAGCCATATCACCACTGGGATACCGATAAGTACGTCACCGGTATCAACGCTATCTCTCACGGCCAGA
  CTACATACGGAAATGCCGAGGACAAGGAATATCAGCAGGGCGTCGGACGCTTTCCTAACGAGAAGGAGCA
  GCTGAAGCAGCTTCAAGGCCTGAATATGCACACATACTTTCCCAATAAAGGCACCCAACAGTACACGGAC
  CAGATCGAGCGCCCACTGATGGTGGGTTCTGTATGGAACAGGAGGGCGCTGCACTACGAAAGCCAGCTGT
  GGAGCAAAATACCTAACCTCGACGACAGCTTCAAAACTCAGTTCGCTGCCTTGGGTGGCTGGGGCCTGCA
  TCAGCCACCACCCCAGATTTTCTTGAAGATCCTCCCACAATCAGGACCTATTGGCGGAATCAAAAGTATG
  GGCATCACCACTCTGGTACAGTATGCCGTGGGGATTATGACCGTGACTATGACTTTCAAGCTCGGCCCAC
  GAAAAGCCACCGGGCGATGGAACCCCCAACCTGGCGTGTACCCACCACACGCAGCCGGGCACCTTCCATA
  CGTGCTGTATGATCCCACCGCAACGGACGCTAAACAACACCACCGCCATGGGTACGAGAAACCTGAGGAG
  CTCTGGACGGCCAAATCAAGGGTGCACCCACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102081 representing YP_004928146
  Red=Cloning sites Green=Tags
  
  MSKESGKWWESDDEFAKAVYQQFVEFYEKVTGTDLELIQILKDHYNISLDNPLENPSSLFDLVARIKNNL
  KNSPDLYSHHFQSHGQLSDHPHALSSSSSHAEPRGEDAVLSSEDLHKPGQVSVQLPGTNYVGPGNELQAG
  PPQSAVDSAARIHDFRYSQLAKLGINPYTHWTVADEELLKNIKNETGFQAQVVKDYFTLKGAAAPVAHFQ
  GSLPEVPAYNASEKYPSMTSVNSAEASTGAGGGGSNPVKSMWSEGATFSANSVTCTFSRQFLIPYDPEHH
  YKVFSPAASSCHNASGKEAKVCTISPIMGYSTPWRYLDFNALNLFFSPLEFQHLIENYGSIAPDALTVTI
  SEIAVKDVTDKTGGGVQVTDSTTGRLCMLVDHEYKYPYVLGQGQDTLAPELPIWVYFPPQYAYLTVGDVN
  TQGISGDSKKLASEESAFYVLEHSSFQLLGTGGTATMSYKFPPVPPENLEGCSQHFYEMYNPLYGSRLGV
  PDTLGGDPKFRSLTHEDHAIQPQNFMPGPLVNSVSTKEGDSSNTGAGKALTGLSTGTSQNTRISLRPGPV
  SQPYHHWDTDKYVTGINAISHGQTTYGNAEDKEYQQGVGRFPNEKEQLKQLQGLNMHTYFPNKGTQQYTD
  QIERPLMVGSVWNRRALHYESQLWSKIPNLDDSFKTQFAALGGWGLHQPPPQIFLKILPQSGPIGGIKSM
  GITTLVQYAVGIMTVTMTFKLGPRKATGRWNPQPGVYPPHAAGHLPYVLYDPTATDAKQHHRHGYEKPEE
  LWTAKSRVHPL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000883
• ORF Size: 2343 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000883.2, YP_004928146
• RefSeq ORF: 2343
• Locus ID: 1489598
• MW: 86.0 kDa