VP2 (NC_000883) Virus Tagged ORF Clone

CAT#: VC102082

Myc-DDK-tagged ORF clone of viral ORF for capsid protein 2 [Human parvovirus B19], codon optimized for human cell expression, YP_004928148

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102082 represents NCBI reference of YP_004928148 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAAGCGTTAACTCTGCGGAGGCTTCCACAGGCGCTGGCGGTGGTGGTAGTAACCCAGTGAAATCTA
  TGTGGTCTGAAGGAGCAACTTTTTCCGCCAACTCCGTGACTTGTACCTTTAGTAGGCAGTTTCTGATCCC
  CTACGACCCGGAGCACCACTACAAGGTTTTCTCCCCCGCAGCCAGTAGCTGTCATAACGCCAGCGGGAAA
  GAGGCGAAGGTGTGTACCATTAGTCCAATTATGGGTTATTCCACCCCCTGGCGCTACCTCGACTTTAATG
  CATTGAACCTGTTTTTCAGCCCACTGGAGTTCCAGCACCTGATTGAAAACTACGGGAGCATCGCACCTGA
  CGCTTTGACCGTTACCATCTCTGAAATTGCTGTGAAAGATGTTACTGATAAGACTGGCGGCGGTGTGCAA
  GTAACTGACTCCACCACTGGGCGGTTGTGCATGCTGGTGGACCACGAGTACAAGTATCCATATGTGTTGG
  GCCAGGGACAGGACACCCTGGCACCGGAACTCCCAATCTGGGTATACTTTCCCCCGCAATACGCTTACCT
  CACCGTTGGCGACGTGAATACCCAGGGAATTAGTGGGGATTCTAAAAAATTGGCCAGCGAGGAGTCAGCC
  TTCTATGTACTGGAGCACTCATCTTTCCAGCTCTTGGGAACGGGCGGTACGGCGACAATGTCATACAAGT
  TCCCCCCAGTACCACCTGAGAATCTCGAGGGATGTTCCCAGCACTTTTACGAGATGTATAATCCACTGTA
  TGGGTCTAGGCTTGGTGTCCCCGACACACTCGGTGGAGATCCTAAGTTTAGATCTCTCACACACGAGGAC
  CATGCTATCCAACCTCAGAACTTCATGCCTGGACCCCTTGTTAATTCTGTCTCCACTAAAGAGGGGGACT
  CTAGCAACACGGGTGCTGGCAAGGCCCTGACCGGCCTGAGTACTGGCACCAGCCAGAATACGAGAATTTC
  CCTTAGACCTGGACCGGTGAGTCAACCATACCATCATTGGGATACCGATAAGTATGTAACTGGGATTAAC
  GCCATCTCCCACGGTCAGACCACCTACGGCAACGCCGAGGACAAGGAATACCAACAAGGAGTGGGCCGGT
  TCCCTAACGAAAAAGAGCAACTGAAGCAGCTGCAGGGCTTGAATATGCATACATATTTTCCTAATAAAGG
  CACCCAGCAGTATACCGACCAGATCGAACGCCCCTTGATGGTTGGTTCCGTCTGGAATAGGAGGGCTCTG
  CATTATGAGTCTCAACTCTGGTCCAAAATCCCAAACCTGGATGACTCTTTCAAGACTCAGTTCGCAGCTC
  TCGGCGGATGGGGACTGCATCAGCCCCCTCCCCAAATTTTCCTGAAGATCCTGCCCCAAAGTGGCCCCAT
  TGGGGGAATCAAGTCCATGGGTATAACCACCCTGGTCCAGTACGCCGTTGGAATCATGACTGTGACGATG
  ACCTTCAAGCTGGGGCCAAGGAAAGCTACAGGCAGGTGGAATCCCCAGCCAGGAGTGTACCCTCCACATG
  CAGCCGGTCATCTGCCTTATGTTCTCTATGACCCCACGGCTACGGATGCCAAACAGCATCACAGACACGG
  ATACGAAAAGCCAGAAGAGCTGTGGACTGCTAAGAGCCGGGTGCATCCTTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102082 representing YP_004928148
  Red=Cloning sites Green=Tags
  
  MTSVNSAEASTGAGGGGSNPVKSMWSEGATFSANSVTCTFSRQFLIPYDPEHHYKVFSPAASSCHNASGK
  EAKVCTISPIMGYSTPWRYLDFNALNLFFSPLEFQHLIENYGSIAPDALTVTISEIAVKDVTDKTGGGVQ
  VTDSTTGRLCMLVDHEYKYPYVLGQGQDTLAPELPIWVYFPPQYAYLTVGDVNTQGISGDSKKLASEESA
  FYVLEHSSFQLLGTGGTATMSYKFPPVPPENLEGCSQHFYEMYNPLYGSRLGVPDTLGGDPKFRSLTHED
  HAIQPQNFMPGPLVNSVSTKEGDSSNTGAGKALTGLSTGTSQNTRISLRPGPVSQPYHHWDTDKYVTGIN
  AISHGQTTYGNAEDKEYQQGVGRFPNEKEQLKQLQGLNMHTYFPNKGTQQYTDQIERPLMVGSVWNRRAL
  HYESQLWSKIPNLDDSFKTQFAALGGWGLHQPPPQIFLKILPQSGPIGGIKSMGITTLVQYAVGIMTVTM
  TFKLGPRKATGRWNPQPGVYPPHAAGHLPYVLYDPTATDAKQHHRHGYEKPEELWTAKSRVHPL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000883
• ORF Size: 1662 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000883.2, YP_004928148
• RefSeq ORF: 1662
• Locus ID: 11293627
• MW: 60.9 kDa