VP1 (NC_015150) Virus Tagged ORF Clone

CAT#: VC102089

Myc-DDK-tagged ORF clone of viral ORF for VP1 [Human polyomavirus 9], codon optimized for human cell expression, YP_004243705

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102089 represents NCBI reference of YP_004243705 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCCCAAAGAAAGAGACAAGAATGCGGCGCATGTCCTGTGAAAAAGACGTGTCCAACTCCTGCCC
  CCGTGCCTAAACTGCTCGTGAAGGGGGGCGTCGAAGTTCTGGAGGTTAGAACGGGACCAGATGCGATCAC
  CCAGATCGAGGCATATCTGAACCCACGGATGGGCAACAACAACCCTACCGACGAGCTGTATGGGTACTCC
  GCGGACATCAACGTCGCTTCCAGCAAAGCAAGCGACAATCCTAATGCAACAACCCTTCCTACATATTCTG
  TCGCTGTAATAAAGCTGCCAATGCTCAACGAAGATATGACATGTGACACCCTGTTGATGTGGGAGGCAGT
  CTCCGTCAAGACCGAGGTTATGGGAATCAGCAGTCTGGTCAACTTGCATCAGGGTGGCAAATATATTTAT
  GGCTCTTCTTCTGGTACCATACCCGTTCAGGGCACTACTCTTCACATGTTTAGTGTCGGCGGCGAACCAC
  TCGAGCTTCAGGGGCTCGTGGCATCTTCCACAACTACATACCCGACCGACATGGTGACTATTAAGAATAT
  GAAGCCCGTGAACCAGGCTCTGGACCCTAATGCTAAGGCGCTTCTGGACAAGGATGGGAAGTATCCGGTG
  GAAGTCTGGAGCCCCGATCCATCCAAAAACGAGAATACCCGCTACTATGGAAGCTTTACAGGGGGCGCCA
  CCACCCCACCAGTCATGCAGTTCACGAATTCCGTGACAACTGTGCTGCTGGATGAGAACGGCGTGGGTCC
  CCTGTGTAAAGGAGACAAGCTCTTCCTTAGTGCGGTGGATATCGTCGGGATCCATACCAATTATTCCGAA
  TCACAGAACTGGAGGGGGCTGCCCCGGTATTTCAACGTTACGCTCAGAAAGAGAGTGGTCAAAAATCCTT
  ATCCCGTTTCAAGTTTGCTCAATTCCCTGTTCTCAGGGCTGATGCCACAGATCCAGGGACAACCAATGGA
  GGGGACATCTGGACAAGTCGAGGAAGTGAGAATCTACCAGGGAACCGAGGGCCTGCCCGGCGACCCCGAC
  TTGGACCGGTATGTGGACAAGTTCTGTCAGAACCAAACCGTGCCCCCCCGAAGTAACGATCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102089 representing YP_004243705
  Red=Cloning sites Green=Tags
  
  MAPQRKRQECGACPVKKTCPTPAPVPKLLVKGGVEVLEVRTGPDAITQIEAYLNPRMGNNNPTDELYGYS
  ADINVASSKASDNPNATTLPTYSVAVIKLPMLNEDMTCDTLLMWEAVSVKTEVMGISSLVNLHQGGKYIY
  GSSSGTIPVQGTTLHMFSVGGEPLELQGLVASSTTTYPTDMVTIKNMKPVNQALDPNAKALLDKDGKYPV
  EVWSPDPSKNENTRYYGSFTGGATTPPVMQFTNSVTTVLLDENGVGPLCKGDKLFLSAVDIVGIHTNYSE
  SQNWRGLPRYFNVTLRKRVVKNPYPVSSLLNSLFSGLMPQIQGQPMEGTSGQVEEVRIYQGTEGLPGDPD
  LDRYVDKFCQNQTVPPRSNDQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_015150
• ORF Size: 1113 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_015150.1, YP_004243705
• RefSeq ORF: 1113
• Locus ID: 10352034
• MW: 40.3 kDa