VP2 (NC_015150) Virus Tagged ORF Clone

CAT#: VC102090

Myc-DDK-tagged ORF clone of viral ORF for VP2 [Human polyomavirus 9], codon optimized for human cell expression, YP_004243703

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102090 represents NCBI reference of YP_004243703 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGGCGTCTTGACCATCCTGCTCGACATCTTCGAACTGGCAGAGCAACTCAGCCTGTCTACGGGGT
  TCACCGTAGACGCAATTCTGAGCGGAGAAGCCATTGCAGCGGCCACTACTGAGGCAGCCTGGCTCATCGA
  GATCGAGGCTGTAGATGTGGCCGGGTTGGGGGCTCTCGAAGCATTGACCCTTACAGGACTCAGCGCAGAG
  GAGTTCTCACTGCTGTCCGCCCTCCCCACAGCCCTGAATAATGCTATCGGGATAGGGATCTTCTTTCAGA
  CTGTCACAGGCGCCAGCGCAGTGGTGGCCGCTGGGGTCACGACATTCGGGTATTCAAAAGAGGTGCCCCT
  GGTGAGCATGGCGCTGACGCCCTGGTTCCCACAGGTAGACTACTTGTTCCCAGGACTGTCCTCTTTCTCC
  TACTATCTGAACGCTGCCCTGGACTGGGGTGAATCACTGTTCCATGCCGTCGGTAGGGAAATTTGGCGGA
  ACATCATGAGACAGGCCACCCAGCAGATTGGGTATACATCCAGAGCTCTGGCAGTACGCGGAACCAACGA
  GTTTCAGCATATGCTGGCACAGATTGCTGAAAACGCACGATGGGCTCTCACTAATGGCCCAATCCACATT
  TACTCCAGCGTCGAAGAATATTATAGGGGGTTGCCCAGTGTTAATCCTATACAGTTGAGGCAGCAATACA
  GGTCTCGCGGCGAGCTGCCTCCTACCAGGGAGCAGTTTGAGTACCAGGAGCAAGTGCGGCTGAGACGGGA
  GATCGGCGGCAGCGAACCTAGATCAGGCCACTATGTCCAGCATTACGCTGCCCCAGGGGGGGCGAACCAA
  AGAGTGAGCCAGGACTGGATGCTCCCACTGATCCTTGGCCTTTACGGGGATATCACCCCAACATGGGAAA
  AGGAACTCTCCAAGCTTGAGAAGGAAGAGTACGGACCACCTAAGAAGAAAGCTCGGGTGAGAAGTATGTC
  CTGTAAAAAGAATTTGTCTAACACACGCAGTCGCTCTCAGACACCATGTCAGAGACGGGGCAGGTCCTCA
  AGGTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102090 representing YP_004243703
  Red=Cloning sites Green=Tags
  
  MGGVLTILLDIFELAEQLSLSTGFTVDAILSGEAIAAATTEAAWLIEIEAVDVAGLGALEALTLTGLSAE
  EFSLLSALPTALNNAIGIGIFFQTVTGASAVVAAGVTTFGYSKEVPLVSMALTPWFPQVDYLFPGLSSFS
  YYLNAALDWGESLFHAVGREIWRNIMRQATQQIGYTSRALAVRGTNEFQHMLAQIAENARWALTNGPIHI
  YSSVEEYYRGLPSVNPIQLRQQYRSRGELPPTREQFEYQEQVRLRREIGGSEPRSGHYVQHYAAPGGANQ
  RVSQDWMLPLILGLYGDITPTWEKELSKLEKEEYGPPKKKARVRSMSCKKNLSNTRSRSQTPCQRRGRSS
  RS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_015150
• ORF Size: 1056 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_015150.1, YP_004243703
• RefSeq ORF: 1056
• Locus ID: 10352036
• MW: 38.9 kDa