VP3 (NC_015150) Virus Tagged ORF Clone

CAT#: VC102091

Myc-DDK-tagged ORF clone of viral ORF for VP3 [Human polyomavirus 9], codon optimized for human cell expression, YP_004243704

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102091 represents NCBI reference of YP_004243704 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTGACGCCTTGGTTTCCTCAGGTGGATTATCTGTTCCCCGGACTGTCCAGCTTTAGTTACTACC
  TGAATGCTGCACTGGATTGGGGCGAAAGTCTCTTCCACGCCGTCGGTCGAGAGATCTGGAGGAACATCAT
  GCGGCAGGCAACACAACAGATTGGCTACACTTCAAGAGCACTTGCCGTTCGAGGAACAAATGAGTTTCAG
  CATATGCTCGCGCAGATCGCGGAAAATGCCCGCTGGGCTCTTACTAACGGACCTATTCACATCTATAGTA
  GCGTCGAGGAGTATTACAGGGGATTGCCTAGCGTGAATCCAATTCAGCTGAGACAGCAGTATAGGTCAAG
  AGGCGAGCTTCCCCCCACCCGAGAGCAATTCGAGTACCAGGAACAGGTGCGCCTGCGGAGAGAAATTGGC
  GGGTCAGAGCCCCGGAGTGGGCACTATGTTCAGCATTACGCAGCCCCTGGCGGGGCTAATCAGAGGGTGA
  GTCAGGATTGGATGCTGCCCCTTATTCTCGGTCTGTACGGGGACATCACACCCACATGGGAGAAAGAGCT
  GTCCAAGTTGGAGAAAGAAGAGTACGGACCCCCCAAAAAAAAAGCTCGGGTGCGGTCAATGAGCTGTAAA
  AAGAACCTGTCCAATACACGCAGCAGGAGCCAGACACCCTGTCAACGCAGGGGGAGATCCTCTCGGTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102091 representing YP_004243704
  Red=Cloning sites Green=Tags
  
  MALTPWFPQVDYLFPGLSSFSYYLNAALDWGESLFHAVGREIWRNIMRQATQQIGYTSRALAVRGTNEFQ
  HMLAQIAENARWALTNGPIHIYSSVEEYYRGLPSVNPIQLRQQYRSRGELPPTREQFEYQEQVRLRREIG
  GSEPRSGHYVQHYAAPGGANQRVSQDWMLPLILGLYGDITPTWEKELSKLEKEEYGPPKKKARVRSMSCK
  KNLSNTRSRSQTPCQRRGRSSRS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_015150
• ORF Size: 699 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_015150.1, YP_004243704
• RefSeq ORF: 699
• Locus ID: 10352033
• MW: 26.9 kDa