large T antigen (NC_015150) Virus Tagged ORF Clone

CAT#: VC102092

Myc-DDK-tagged ORF clone of viral ORF for large T antigen [Human polyomavirus 9], codon optimized for human cell expression, YP_004243706

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: large T antigen
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102092 represents NCBI reference of YP_004243706 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCAGACCCTGTCCCTGGAAGAAAGGAACGAACTGATGGACTTGCTCCAGCTGACCCGAGCAGCCT
  GGGGAAACCTGTCTCTTATGAAGAAGGCCTACAAGACCGTGTCTAAAATCTATCACCCCGATAAAGGCGG
  AAACCCTGAGAAAATGCAACGGCTGAACGAGCTTTTTCAGAAACTGCAAGTGACCCTGCTGGAAATCAGA
  AGTAACTGCGGTAGTAGCTCCTCCCAGGGCTATTATTCAGACTCTCCATACTTCACAGAGACTCCCTTTT
  CATATTGCGAGCGAAAGAACGAGGATCCAGAGGGAGGCTCTTGGGGCAAGTGGTGGCGCGAGTTCGTTAA
  TAAGGAATATGACGACCTCTTTTGCTCCGAGACTATCAGTTCTAGTGACGACGAAAACAATCCTGGCCCA
  AGCGCTCCACCACCATCTAGTGCCTCAGCAAGTGAGGATCCAGATCCTGAAGAGGAAGCCGGCTCCTCCC
  AGTCTTCCTTCACATGCACCCCGCCAAAAAGAAAAAAACCTGAACCCAACACTCCTGAGGATTTTCCCAT
  GTGTCTTTATAGCTTCCTGTCCCACGCTATATACTCCAACAAAACCATGAATTGTTTCCTCATCTATACC
  ACCGTCGAGAAATCTAAGCAGCTGTACCGCACCGTAGAGAAGTCAAAGATTAAGGTCGACTTTAAAGCCA
  TCTTCCTGTACAAAGACGATGGTATCGAGGGAGGACTGCTGTACTTCATCACCCTGGGGAAACATAGAGT
  GAGCGCGGTGAAGCACTTTTGTGTCGCACAGTGTACTTTTAGCTTTATCCATTGTAAAGCCGTGATAAAA
  CCACTCGAGCTGTATCGGGCTCTCGGTAAGCCCCCTTTCAAGTTGCTGGAGGAGAATAAGCCCGGCGTAA
  GTATGTTCGATTTCCAGGAGGAAAAGGAGCAGGCAGTTAACTGGCAGGAGATTTGTAACTATGCTGTGGA
  GGCTAAGATAACCGATGTGCTCCTTTTGCTGGGGATTTATCTCGACTTTGCTGTAGAACCAGGCACCTGC
  TCAAAGTGCGAAAAGAAGTCACACAAATTCCACTACAATTATCACTCAAAACATCATGCAAACGCTTGTC
  TTTTTCTCGAGAGCAAGAGCCAGAAAAATATCTGTCAACAGGCCGTTGATCAGGTTCTCGCAGCGAAAAG
  GTTGAAGTTGGTAGAATGTACAAGAATGGAGTTGTTGGAGGATAGATTTATTCAGCTTTTTGACGAGATG
  GAAGACTTTCTCCACGGCGAGATTGAAATCCTTAGATGGATGAGCGGCGTAGCGTGGTATACAATACTGC
  TCGACAATTCATGGGATGTATTTCAGAAGATCCTGCAGCTTGTGACTACCAGTCAGCCAAAGAAACGCAA
  TATCCTGTTTAAAGGTCCAATCAACTCCGGTAAAACTACACTCGCTTCAGCTTTTATGCATTTCTTCGAC
  GGCAAAGCTCTGAACATAAATTGCCCGGCGGAGAAACTGTCCTTCGAGCTGGGATGCGCCATAGACCAGT
  TCTGCGTGTTGCTCGACGACGTAAAAGGCCAAATAACCCTCAACAAACATCTCCAGCCAGGGCAGGGAGT
  CAACAATCTGGATAACCTTAGAGACCATCTTGACGGGACAATTAAGGTTAACCTTGAAAAAAAGCACGTA
  AATAAACGGTCACAGATCTTTCCCCCGGTCATCATGACTATGAATGAATATCTCCTGCCTCCAACCGTCG
  GGGTGCGCTTTGCCTTGCACATCCACTTCCACTGCAAAACATACCTGAAACAGTCCCTGGAGAAATCAGA
  CCTTATTGAGAAAAGGATACTGAACTCCGGCTACACAATCCTTCTCCTGCTGTTGTGGTACAACCCTGTG
  GACAGCTTCACGCCCAAGGTGCAAGAGTACGTGGTGAAATGGAAAGAGATTCTCGAACGCCATGTTTCAA
  TCACACAGTTCGGGAATATTCAACAAAATATTCTGGATGGCAAGGATCCCCTGCACGGTATTGTGATCGA
  AGAGCAGGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102092 representing YP_004243706
  Red=Cloning sites Green=Tags
  
  MDQTLSLEERNELMDLLQLTRAAWGNLSLMKKAYKTVSKIYHPDKGGNPEKMQRLNELFQKLQVTLLEIR
  SNCGSSSSQGYYSDSPYFTETPFSYCERKNEDPEGGSWGKWWREFVNKEYDDLFCSETISSSDDENNPGP
  SAPPPSSASASEDPDPEEEAGSSQSSFTCTPPKRKKPEPNTPEDFPMCLYSFLSHAIYSNKTMNCFLIYT
  TVEKSKQLYRTVEKSKIKVDFKAIFLYKDDGIEGGLLYFITLGKHRVSAVKHFCVAQCTFSFIHCKAVIK
  PLELYRALGKPPFKLLEENKPGVSMFDFQEEKEQAVNWQEICNYAVEAKITDVLLLLGIYLDFAVEPGTC
  SKCEKKSHKFHYNYHSKHHANACLFLESKSQKNICQQAVDQVLAAKRLKLVECTRMELLEDRFIQLFDEM
  EDFLHGEIEILRWMSGVAWYTILLDNSWDVFQKILQLVTTSQPKKRNILFKGPINSGKTTLASAFMHFFD
  GKALNINCPAEKLSFELGCAIDQFCVLLDDVKGQITLNKHLQPGQGVNNLDNLRDHLDGTIKVNLEKKHV
  NKRSQIFPPVIMTMNEYLLPPTVGVRFALHIHFHCKTYLKQSLEKSDLIEKRILNSGYTILLLLLWYNPV
  DSFTPKVQEYVVKWKEILERHVSITQFGNIQQNILDGKDPLHGIVIEEQA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_015150
• ORF Size: 2040 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_015150.1, YP_004243706
• RefSeq ORF: 2040
• Locus ID: 10352032
• MW: 78.0 kDa