small T antigen (NC_015150) Virus Tagged ORF Clone

CAT#: VC102093

Myc-DDK-tagged ORF clone of viral ORF for small T antigen [Human polyomavirus 9], codon optimized for human cell expression, YP_004243707

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: small T antigen
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102093 represents NCBI reference of YP_004243707 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCAGACCCTGTCACTGGAGGAGCGCAATGAGCTGATGGACCTCCTGCAACTCACTCGCGCTGCTT
  GGGGCAACCTGTCCCTGATGAAGAAGGCTTATAAAACTGTGTCCAAAATTTACCACCCAGATAAAGGAGG
  CAATCCTGAAAAGATGCAGCGCTTGAACGAGTTGTTCCAGAAGCTCCAAGTGACCCTGTTGGAAATCCGC
  TCCAACTGTGGTTCTTCAAGCTCTCAGGTAGCCTGGTATTTTTGGGACGAGAATTTTCGAACACTTGGCG
  CATTCCTCGGCGAAAAATTCAATCAGAGAATTATCGGGGGATACCCAGATTGTATCACCTATAATAAGCC
  TTCCTGCTGCTGCATAGTGTGCCTGCTCAAACAGCAGCACAAGTCAACCAAAATCAACAAAAAGAAACCC
  TGCCTCGTCTGGGGTGAGTGCTTTTGCTACAAGTGCTACCTGCTGTGGTTTGGGTTTCCTGAAGACTTTA
  CCTCCTTCAATTACTGGACACTTCTGATGAGAAATATGGACCTCAGCCTTCTGAGACTGTGGACCGAGCT
  CGGGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102093 representing YP_004243707
  Red=Cloning sites Green=Tags
  
  MDQTLSLEERNELMDLLQLTRAAWGNLSLMKKAYKTVSKIYHPDKGGNPEKMQRLNELFQKLQVTLLEIR
  SNCGSSSSQVAWYFWDENFRTLGAFLGEKFNQRIIGGYPDCITYNKPSCCCIVCLLKQQHKSTKINKKKP
  CLVWGECFCYKCYLLWFGFPEDFTSFNYWTLLMRNMDLSLLRLWTELGF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_015150
• ORF Size: 567 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_015150.1, YP_004243707
• RefSeq ORF: 567
• Locus ID: 10352035
• MW: 22.2 kDa