putative NTPase (NC_003494) Virus Tagged ORF Clone

CAT#: VC102353

Myc-DDK-tagged ORF clone of viral ORF for putative NTPase [Infectious spleen and kidney necrosis virus], codon optimized for human cell expression, NP_612285

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: putative NTPase
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102353 represents NCBI reference of NP_612285 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGTGCTGCTCAGCTTGTATCCATCACTCTCTGATCGACATTTCAGTGTGCATGGCGACATCAGGC
  TGACTGGCGGCCTCGATCCCGCAGACGATTTCGTCCAGCAGCAGTACCTGGCTCGCTTCTTGAGCCCAGA
  GACCCCTTATAATGAGCTCCTGCTTTTCCACGAGATGGGCACCGGAAAGACCTGCACCGCAGTGGCCATA
  GCACAATGCGCTGCCAAATACCTCAAAGGTGTGCTGGTGATCACCCGGGGAGAAGGGCTTATGCGGAATT
  TCCAGTCAGAGATAATGCACAAATGCCACTCTGTTTGGAGACGGGAGAGGAGGTCAATCGAACAATTCTT
  TCGGTTTGAGACCTACGATCGCTTCGCAGCCTGGTGTGGGCGCGTGTCCCGAGGGGTGCTGGCTACAAAA
  TATAGGAATCACCTCGTCATCGTCGACGAAGTACATAATCTGCGCGAGGATAATGCTTCATACAGGGCAC
  TGCTCGAATTCATGCATACAGTGCAGGGTGCCAAAAAAGTGCTTCTCTCAGGCACGCCTATGTCAGATCG
  GGCCGACGAGATTATAGACGTAATGAACTTGATTCTGCCCCCCGATGCTACTATGGATAAGGCCCATTAC
  TTCACTGCCGGCGGGGTGTTTGTCGACGGGCGAGAGTCCGAATTTATGGCCGCAGTCCGGGGGAGGGTAT
  CTTTTGTACTGGCCAAAGACTCAGGCGTGCGAGTCGTCTACGCCGGCGCCGCTGTGGATGGACTGCCTTT
  TAACGTGGTGCAGTTGGCTATGAGAAAGTATCAGCGGCGGGCCTACCGCAGGGCTGTGCGCGAAGACGCG
  GAGGCTAACACTATCTTCACAAGTACGAGGAAGGCTTCTTTGGCGGCAATGCCAGCCGAAGGCCAGCTCG
  TCGAGTATAGTGTGAAATATGATTACGTCCTCAGGAGCTTGGAGCGCGCTAGGAAGGCTTTTGTATACTG
  CGATGTGGTACGAGGCACTGGAGTGACGATGCTGGCGGACATCCTTCAGCGGAACGGGTGGCACATGGTT
  ACGAGCAGCAAATTCAACGGCGTACGGAGAGCGAAGCGATTTATCGTCCTTACTGGGGCAGAGTCTGCCA
  GAGCGCACAAGATGTTGGAGCGGTTCAATCACGAAAGAAATGTGGACGGGTCTGACATTTCTCTGGTCAT
  TGGATCCAGGGCAGTGGCTGAGGGCTTCACCATGCGGGATGTGACTGATGTTTTGGTCTTGACCCCACAC
  TGGAACTTTACCGAAACATTTCAAGCAATCTACCGCGCAGTGAGGGCACGGAGCCACGAGTACACCCGCA
  CACTTCTTGGACAACCTGATCCTCAGGTGACAGTGCACATGGTCGTGGCACTTTGCTCAGAGGAGTCCCT
  TGACCTGAATATGCTCAGGACCGCCATGGCTAAAGACGTCCAGATTAAAAGGGTCGAATATATGCTTAAA
  GTGGCCGCTGTCGATTGTGGATTTATGAAAGCCAGAAACATGAGACACACTGGCGACGGGTCCCGGGAGT
  GCCAGTACACGGTTTGTGAGTACCGCTGTAAGGGGCAGCGAACGGCCGAGCGGAGGAGGAGGCACTTTAT
  CCCAGTGGTGCCCAGTGAGAGAGCCAGACTCAACGCCGTCCAGGGTTCATCATTGATCGGATGGGGGGGC
  GAGGACGTACCACAACTCTGGTACATGTGGGCACACCGCGTCCCTGTCCGGAATTTTTCTGGGCATATCG
  CATATATGGTACCATCAATGATTAACGGCAACACCAGTATGTTGGTGACTACCGACCCCTATGCCACCGG
  TCTGACCGTTGAAGACGAATACTATGCTAACAACTTTGTGTGTAGCAACGAGCACGCTTTTACACTGGCA
  CAGCTGTGCGAGAGAAGGGACGCCAGAGCCGCACCAAGAATTGTGGCGAGGATTCTGGACGGGACTGACG
  GTCCTGAATGCATGCTGCGACTGCCGAGAGCGTCCCAACTCAAGGTCCTGAAGGCCCTGGTGCAGATGTA
  CGCCGAGCACGAAGCTCTCGACGAGCACCAGAGTGGAGTGCTCGACATGTACCGCGGATTTTGGGACCCT
  GAAAGTTACCAGCTCTGGCTGTACGAAGCTGATTTGGTACTCCAAAGATCCGACGCAACTGGGATGCTGC
  AATGGGGCACAGACCCTGCAAAGAGAGGAACCAGGCGCAAGCAGGAGTTGATGGCTTCTCCAGTTGGATA
  CTACGGTATGTACAACCCTGCCCGGGGGGATTTCTGCATTCGGGACGTGAGAGGGGCGGCCGACGGAGGG
  GATATGCGGCGACTGAAAGTCGGTCAGAGGTGCGTTGATATGGACAAGTGGACACTCCTGCACCTCCTGG
  TGACTAGGCTGAAACCTCGCGACGTTCCAGATTCACACCGGAGCACCCGCCGCTTGATGGCGATGTGCAT
  GGACATCGCACGGGCACAACGCGCACATAACGTGATTCGGAACTTTGTGCCGGCCGATAGACGAGAAGCT
  GTCTTGTTTGTACACTATGTGGACATGTCTCGAGAGCAACTCTGCAAAGTCATCAAAAGGTGGATGAGGC
  GACACGGACTGCTCGAGCACAACTTTGAGTGCGGGCACGCTTTCAAACACAGGGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102353 representing NP_612285
  Red=Cloning sites Green=Tags
  
  MDVLLSLYPSLSDRHFSVHGDIRLTGGLDPADDFVQQQYLARFLSPETPYNELLLFHEMGTGKTCTAVAI
  AQCAAKYLKGVLVITRGEGLMRNFQSEIMHKCHSVWRRERRSIEQFFRFETYDRFAAWCGRVSRGVLATK
  YRNHLVIVDEVHNLREDNASYRALLEFMHTVQGAKKVLLSGTPMSDRADEIIDVMNLILPPDATMDKAHY
  FTAGGVFVDGRESEFMAAVRGRVSFVLAKDSGVRVVYAGAAVDGLPFNVVQLAMRKYQRRAYRRAVREDA
  EANTIFTSTRKASLAAMPAEGQLVEYSVKYDYVLRSLERARKAFVYCDVVRGTGVTMLADILQRNGWHMV
  TSSKFNGVRRAKRFIVLTGAESARAHKMLERFNHERNVDGSDISLVIGSRAVAEGFTMRDVTDVLVLTPH
  WNFTETFQAIYRAVRARSHEYTRTLLGQPDPQVTVHMVVALCSEESLDLNMLRTAMAKDVQIKRVEYMLK
  VAAVDCGFMKARNMRHTGDGSRECQYTVCEYRCKGQRTAERRRRHFIPVVPSERARLNAVQGSSLIGWGG
  EDVPQLWYMWAHRVPVRNFSGHIAYMVPSMINGNTSMLVTTDPYATGLTVEDEYYANNFVCSNEHAFTLA
  QLCERRDARAAPRIVARILDGTDGPECMLRLPRASQLKVLKALVQMYAEHEALDEHQSGVLDMYRGFWDP
  ESYQLWLYEADLVLQRSDATGMLQWGTDPAKRGTRRKQELMASPVGYYGMYNPARGDFCIRDVRGAADGG
  DMRRLKVGQRCVDMDKWTLLHLLVTRLKPRDVPDSHRSTRRLMAMCMDIARAQRAHNVIRNFVPADRREA
  VLFVHYVDMSREQLCKVIKRWMRRHGLLEHNFECGHAFKHRA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003494
• ORF Size: 2646 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003494.1, NP_612285
• RefSeq ORF: 2646
• Locus ID: 935386
• MW: 100.7 kDa