(NC_003494) Virus Tagged ORF Clone

CAT#: VC102401

Myc-DDK-tagged ORF clone of viral ORF for putative tumor necrosis factor receptor associated factor [Infectious spleen and kidney necrosis virus], codon optimized for human cell expression, NP_612333

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102401 represents NCBI reference of NP_612333 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGTGTCAGCCAAACAACAGACTGCTTTGTGCCACATGCAAGGGTATCATGCGCGGACCTGTGC
  AAGCTGCCTGCGGGCATAGATTTTGTGAAGGATGTCTCACTGAGAGCGTGTGTCGGCACTGTTTGTGTAC
  TACCGCGCCCGTCTTCAGGGATAATGCTGTTGCTCACGAGCTGGTTGCCGCAGTGGTGAGATGTGTGGTG
  TGTCCATGGGTTGGAACAGTGGCCGAATACAGGGAGGGCCACGTCATGGAGCACGAGGGAATGCAGCCCT
  GCCCCTTTGCAGTCTTGGGATGCAATACGGATGACTGCGATGATACCGTTGAGGACCACCAGGAGCTGCT
  GGTGAAGTGTTTGAAACGAGCAACTGACAGGATTAAGGCCCTCGAAGCTAGCGTGAACAACCTGAATATG
  GAGCGCCAGGCCATAGCTGCCAGGTTTGCTCATCTGGAGATGGCAACTCAAAACGGTACTCTTGTCTGGC
  TGGTGGCCGTGAGCTCCGACTGTCCGTTGCCGATGGTCCTCAGCCCCGTCTTCTATACACACCGGAATGG
  CTACGCTATGTGTGTCAGAATGTACCCAAAGGGCGACGGGAGCGGGACTGGAACACACGTTAGCATGTAT
  TTCAATGTACTCAAGGGACCAGCGGATGATACCCTGGAGTGGCCTTTCTCCAAACACGTTACCGTGTCTC
  TGGTGGGACAGGAGCCCGGAGTGCCCGACATCGTGCACAGCTTCCGGTCCAACTTTGATTCTGCATCTTT
  CAAGAAACCCGTTGGCGCAAATATTCCGATTGGCTGGCCAACCTTCTGCCCACTCACCTGTGTTCCCATG
  TATACTAAGAATGGCCAACTGTGCATTAAGGTCCATGTGTGTGATTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102401 representing NP_612333
  Red=Cloning sites Green=Tags
  
  MELCQPNNRLLCATCKGIMRGPVQAACGHRFCEGCLTESVCRHCLCTTAPVFRDNAVAHELVAAVVRCVV
  CPWVGTVAEYREGHVMEHEGMQPCPFAVLGCNTDDCDDTVEDHQELLVKCLKRATDRIKALEASVNNLNM
  ERQAIAARFAHLEMATQNGTLVWLVAVSSDCPLPMVLSPVFYTHRNGYAMCVRMYPKGDGSGTGTHVSMY
  FNVLKGPADDTLEWPFSKHVTVSLVGQEPGVPDIVHSFRSNFDSASFKKPVGANIPIGWPTFCPLTCVPM
  YTKNGQLCIKVHVCDL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003494
• ORF Size: 888 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003494.1, NP_612333
• RefSeq ORF: 888
• Locus ID: 935406
• MW: 32.5 kDa