putative ribonuclease (NC_003494) Virus Tagged ORF Clone

CAT#: VC102377

Myc-DDK-tagged ORF clone of viral ORF for putative ribonuclease [Infectious spleen and kidney necrosis virus], codon optimized for human cell expression, NP_612309

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: putative ribonuclease
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102377 represents NCBI reference of NP_612309 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTAAGGCTTTCATCGTCGACTTTATCCTGGTGCGCTGTTGCGAGATGGACAGGGCTTTTGCGCAGG
  CTATGTACGATAAGAACACCCAGGTATTTCACAGCGTTTTTACCCACTCCAAAGCGCATCCCGTGAATAA
  TTATGAGTGGTACGAGAAGTTGGGCGATGCAATTATCGCCTCTGTGATGCCCCAGTACTACATGGAGCGA
  TTCCCCAAGCTCGCACACGACCCTAGCGCAACGGTAGCCAGGACAGTCTCACAACTCGCAATTACTTACC
  AAAGTGGCACCACAATGTCCAATATTGCCCAGAGGATGGGTTTCCTGAAGTGGGTTGTTGCCCATCGAGA
  TGGCGTTGCCCCCGCATCTTATAAGGAGCAGCAGCGGCTCCTGGAGGACACCTTCGAGGCAATGGTGGGT
  GCCATTAGCATTACAATGGACAATGCTACAGGAGTGAAGTGTAGCGGCCATATTGCGTGCTACAGATTCT
  TGAAGTATGCATACGGATTGATCAATATGAGACTGGACGGACTGATCGATAACAAATCAATGCTCAAGGA
  ACTGATCGATATGCATTCATCTGTACTGGACAGTTTCACTTTCGAATACACGAATAACGTCATGACACTC
  ACGGTAGCCCACGGCAAGTATACAGTGAGCGAAGCCAACGTGAACAACCTGAGTAAGAGAGAACTCGAAA
  AAGTACTCGCCGGCAAGGTGCTGGCTCAATTGGCTTCCGATGGATATGTGCACAAGTCTATGAGACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102377 representing NP_612309
  Red=Cloning sites Green=Tags
  
  MFKAFIVDFILVRCCEMDRAFAQAMYDKNTQVFHSVFTHSKAHPVNNYEWYEKLGDAIIASVMPQYYMER
  FPKLAHDPSATVARTVSQLAITYQSGTTMSNIAQRMGFLKWVVAHRDGVAPASYKEQQRLLEDTFEAMVG
  AISITMDNATGVKCSGHIACYRFLKYAYGLINMRLDGLIDNKSMLKELIDMHSSVLDSFTFEYTNNVMTL
  TVAHGKYTVSEANVNNLSKRELEKVLAGKVLAQLASDGYVHKSMRL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003494
• ORF Size: 768 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003494.1, NP_612309
• RefSeq ORF: 768
• Locus ID: 935341
• MW: 28.9 kDa