putative RNA guanylytransferase (NC_003494) Virus Tagged ORF Clone

CAT#: VC102354

Myc-DDK-tagged ORF clone of viral ORF for putative RNA guanylytransferase [Infectious spleen and kidney necrosis virus], codon optimized for human cell expression, NP_612286

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: putative RNA guanylytransferase
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102354 represents NCBI reference of NP_612286 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCTCCGCACGCTTGGGTTTCATGTCCCGCACAGGGCACCGAGATCTGCGGCTTTATCACCCCCG
  GTAAGACATTCCTGGATAGTCGGTATGATACTTATATAGCCGCTGAAGCACATTACCGCCCCCCCCTTCC
  CGACATGTACTCCGCAGTAATAGATCTGACTAATACCGCCAGATACTACAACGGACGCGCCTTGGGCGCA
  TGCTACCACAAGATTAAATGCAAAGGACACAATCAGTGCCCGTCTCCCAGAGCTGTTAAGGCCTTCATCG
  ACACCGTCGTAGCGGCCCCCGGGTTGGTGTACGTCCACTGTACCTATGGGTTTAACCGGACTGGGTATTT
  GATTTGCTGCTACCTGGTGGAGTGTCGGAAAATGTCCGTGCACGATGCCATCCGACTGTTCGCCGAAGCT
  AGACCTCCAGGAATGTATAAAGCCGATTACATTAAGACGCTGTGTGTAAAATACAACGCAAGGGTCCTGG
  CCGTCCCTAGCAATCTTCCGAGTTGGCTCCCCGAGCAAGTTGTTGTGACCCACACCGCCTCCGGCCCAAA
  AGCAGCTCGGCACGTGCTGAGATGCATCTCTGTCGAGGATCCCGTGCTCGCCGCCGAGATCGTGAGAGTG
  GTCGCGACCACGGTTGGATACCCCTACACCGACCAGTTCGCCGGTTGTATGCCCGTGTCAATGTCTAAGC
  ACAATTACGCTAGTATCCTGTGCCTGAAGGACGATGCGCGGGCCACCTGGAAGGCTGACGGCGTTAGGTA
  CATGATGTATATCGACGGTAAGGACCGCGTCTATGTGATAGATCGCTGCGCCCACGTATCCCGGCTGACC
  GCCGACGTTTTCGTGGGGCCAGATGGAATGCACCTTGTTGATACCCTGGTGGATTGCGAGTATACCTCAA
  ACGGCACAATGTACATTTTCGACGCAGTATACGTGAGAGGAGTTTATGTGTGCGATGCTCCACTGGACTC
  ACGGCTGGCTAGCGTCCAGTGTGTGGCTCAGGCCCATCGGGGTCCCATGTCTGTTAGGGTCAAATGCTCT
  GTAGGAATTGGCGATTTTGAGCTGCTGTACTCTACCTATGCAGATCGAAGCGACTGCGATGGATTCATCT
  TTCAGTCAAACAGCCGGCCATACTACGGCGGTCGGGATGTGCAGATCATCAAGTACAAGCCTGTACACAA
  GAATACTGTTGACTTTATGCTCGTTCCATCCGATGCACAGTGCGCCGGGTTGTATCTCCACAATGATACA
  ACACCATTTGCACAGGTCAGCGCCTGGGATCCAGCGATGGCAGGTAGAGTAGTGGAGTGTGCGTATGTCA
  ATGGTACTTGGAGTGTACTGAGGGTTCGGGCCGACAAATCACAGGCGAATACGAGGGTGACAGCTCTGCG
  GGTTTATGAGTCTATTATCCACCCTGTTGACTTCGATCTCTTTCGGGAAACGATTAGCCTTGCCATGCTG
  TTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102354 representing NP_612286
  Red=Cloning sites Green=Tags
  
  MLPPHAWVSCPAQGTEICGFITPGKTFLDSRYDTYIAAEAHYRPPLPDMYSAVIDLTNTARYYNGRALGA
  CYHKIKCKGHNQCPSPRAVKAFIDTVVAAPGLVYVHCTYGFNRTGYLICCYLVECRKMSVHDAIRLFAEA
  RPPGMYKADYIKTLCVKYNARVLAVPSNLPSWLPEQVVVTHTASGPKAARHVLRCISVEDPVLAAEIVRV
  VATTVGYPYTDQFAGCMPVSMSKHNYASILCLKDDARATWKADGVRYMMYIDGKDRVYVIDRCAHVSRLT
  ADVFVGPDGMHLVDTLVDCEYTSNGTMYIFDAVYVRGVYVCDAPLDSRLASVQCVAQAHRGPMSVRVKCS
  VGIGDFELLYSTYADRSDCDGFIFQSNSRPYYGGRDVQIIKYKPVHKNTVDFMLVPSDAQCAGLYLHNDT
  TPFAQVSAWDPAMAGRVVECAYVNGTWSVLRVRADKSQANTRVTALRVYESIIHPVDFDLFRETISLAML
  F
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003494
• ORF Size: 1473 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003494.1, NP_612286
• RefSeq ORF: 1473
• Locus ID: 935415
• MW: 54.7 kDa