HMGA1 (NM_145899) Human Mass Spec Standard

CAT#: PH302928

HMGA1 MS Standard C13 and N15-labeled recombinant protein (NP_665906)

Product Images

Coomassie blue staining of purified HMGA1 protein (Cat# TP302928). The protein was produced from HEK293T cells transfected with HMGA1 cDNA clone (Cat# RC202928) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC202928
• Predicted MW: 11.7 kDa
• Protein Sequence:
  Sequence Link

  >RC202928 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSESSSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQKEPSEVPTPKRPRGRPKGSKNKGAA
  KTRKTTTTPGRKPRGRPKKLEKEEEEGISQESSEEEQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_665906
• RefSeq Size: 2031
• RefSeq ORF: 321
• Synonyms: HMG-R; HMGA1A; HMGIY
• Locus ID: 3159
• UniProt ID: P17096
• Cytogenetics: 6p21.31
• Summary: 'This gene encodes a chromatin-associated protein involved in the regulation of gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of AT-rich regions in double-stranded DNA. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been identified on multiple chromosomes. [provided by RefSeq, Jan 2016]'
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Stem cell relevant signaling - JAK/STAT signaling pathway, Transcription Factors