Secondary Antibodies

View as table Download

Human IgD (heavy chain) goat polyclonal antibody, Purified

Applications ELISA.
Reactivities Human
Immunogen Human IgD paraproteins.

Rabbit IgA goat polyclonal antibody, AP

Applications ELISA: 1/100-1/1000.
Reactivities Rabbit
Conjugation AP
Immunogen Purified Rabbit IgA

Rabbit IgA goat polyclonal antibody, HRP

Applications ELISA: 1/10000-1/100000.
Reactivities Rabbit
Conjugation HRP
Immunogen Purified rabbit IgA

Turkey IgG (H+L chain) goat polyclonal antibody, AP

Applications ELISA: 1/2,000-1/4,000.
Conjugation AP

Turkey IgG (H+L chain) goat polyclonal antibody, HRP

Applications ELISA: 1/4,000-1/8,000.
Conjugation HRP

Rat IgG2ab (subclass specific) goat polyclonal antibody, Serum

Applications Immunoprecipitation.
In precipitating techniques as Immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG2a and/or IgG2b in Rat serum and other body fluids or to determine its concentration.
To prepare an immunoadsorbent for the purification of Rat IgG2a/IgG2b from serum or plasma.
Recommended Working Dilutions:
Immunoelectrophoresis: 2 µl or equivalent against 120 µl antiserum.
Double radial immunodiffusion (Ouchterlony): a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre not less then 1/64 when tested against pooled normal Rat serum in agar block immunodiffusion titration.
Reactivities Rat
Immunogen Pools of purified homogenous IgG2a and IgG2b isolated from Rat serum. 
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgA + IgG + IgM (H+L chain) goat polyclonal antibody, Serum

Applications Can be used in immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins; as secondary antibody to precipitate the immunoglobulin in normal monkey serum. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
Directions for use:
In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum.
In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Reactivities Monkey
Immunogen Purified normal IgG, IgA and IgM fractions isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgG (H+L chain) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in normal monkey serum; to prepare an immunoadsorbent for the production of Ig-free monkey serum, plasma or non-Ig preparations.
Directions for use:
In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum.
In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Reactivities Monkey
Immunogen Purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

PrecipHen® (Chicken IgY) goat polyclonal antibody, Agarose

Applications Immunoprecipitation.
Reactivities Chicken
Conjugation Agarose
Immunogen Immune sera was collected from goats that were hyper-immunized with purified chicken IgY.
Production: Goat anti-Chicken IgY antibodies were affinity purified from the sera over a Chicken IgY column, and then conjugated to N-hydroxysuccinimide (NHS)-activated Agarose beads. After blocking residual NHS sites, the agarose beads were washed exhaustively with 50mM HEPES buffer (pH 7.6) with 5mM EDTA (TE buffer). Sodium Azide was then added to a final concentration of 0.02% (w/v).

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, FITC

Applications Immunocytochemistry (1/200).
In this latter test, abundant Fluorescence staining was observed in 3T3 cells after blocking with BlokHen® (1/10 dilution in PBS), incubation with Chicken anti-Vimentin antibodies (1/1000 dilution in PBS) and then incubation with GGHL-30F-2 (1/200 dilution in PBS).
Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After electrophoresis was complete, Goat anti-Chicken serum (75 μl) was placed in the lower trough and GGHL-30 (75 μl) (pre-conjugated affinity-purified antibody) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough.
Reactivities Chicken
Conjugation FITC
Immunogen Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected.

Human IgA + IgG + IgM (F(ab)2 Fragment) goat polyclonal antibody, Aff - Purified

Applications ELISA.
Reactivities Human

Mouse IgG3 goat polyclonal antibody, Purified

Applications ELISA.
Reactivities Mouse
Immunogen Mouse IgG3 paraproteins.

Mouse IgG goat polyclonal antibody, FITC, Aff - Purified

Applications Immunofluorescence: 1/500-1/1000. 
Immunohistochemistry.
Reactivities Mouse
Conjugation FITC
Immunogen Purified Mouse IgG fraction

Monkey IgA + IgG + IgM goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/500-1/4,000.
Western Blot: 1/500-1/2,500.
Immunohistochemistry: 1/200-1/1,000.
Conjugation AP
Immunogen Monkey IgG, IgA and IgM whole molecule.

Monkey IgA + IgG + IgM goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation FITC
Immunogen Monkey IgG, IgA, IgM whole molecules.

Monkey IgA + IgG + IgM goat polyclonal antibody, Aff - Purified

Applications Immunoprecipitation.
Immunodiffusion.
ELISA: 1/100000.
Western Blot: 1/2000 - 1/10000.
Immunogen Monkey IgG, IgA and IgM whole molecules

Monkey IgA + IgG + IgM goat polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Monkey IgG, IgA and IgM whole molecule.

Monkey IgA + IgG + IgM goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Monkey IgG, IgA and IgM whole molecule.

Chicken IgG / Chicken IgY (H+L chain), adsorbed goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions: This product has been assayed against 1.0 µg of Chicken IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product.
Conjugation AP
Immunogen Chicken IgG, whole molecule.

Chicken IgG / Chicken IgY (H+L chain), adsorbed goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended dilutions: This product has been assayed against 1.0 ug of Chicken IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:8,000 to 1:36,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Chicken IgG whole molecule.

Chicken IgG / Chicken IgY (H+L chain), adsorbed goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Flow cytometry: 1:2,000 - 1:10,000.
IF Microscopy: 1:500 - 1:2,500.
Conjugation FITC
Immunogen Chicken IgG whole molecule

Chicken IgG / Chicken IgY (H+L chain), adsorbed goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions:
ELISA: 1/10,000-1/50,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Conjugation HRP
Immunogen Chicken IgG whole molecule.

Chicken IgG / Chicken IgY (H+L chain), adsorbed goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Immunogen Chicken IgG whole molecule.

Chicken IgG / Chicken IgY (H+L chain), adsorbed goat polyclonal antibody, TRITC

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Chicken IgG whole molecule

Chicken IgG / Chicken IgY (H+L chain), adsorbed goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Chicken IgG whole molecule.

Guinea pig IgG (H+L chain), adsorbed goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Guinea Pig IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.
A working dilution of 1:1,000 to 1:5,000 is suggested for this product.
Conjugation AP
Immunogen Guinea Pig IgG whole molecule.

Guinea pig IgG (H+L chain), adsorbed goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Guinea Pig IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry: 1:1,000 - 1:5,000, Western blotting: 1:2,000 - 1;10,000.
Conjugation Biotin
Immunogen Guinea Pig IgG whole molecule.

Guinea pig IgG (H+L chain), adsorbed goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
A dilution of 1:50,000 is suggested for ELISA.
Immunogen Guinea Pig IgG whole molecule.

Guinea pig IgG (H+L chain), adsorbed goat polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Guinea Pig IgG whole molecule.

Guinea pig IgG (H+L chain), adsorbed goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Guinea Pig IgG whole molecule.

Hamster IgG (H+L chain), adsorbed goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Hamster IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:6,500 of the reconstitution concentration is suggested for this product.
Conjugation AP
Immunogen Hamster IgG, whole molecule.

Hamster IgG (H+L chain), adsorbed goat polyclonal antibody, Biotin

Applications Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Western blot: 1:1,000 - 1:5,000.
ELISA: 1:10,000 - 1:50,000.
Immunohistochemistry.
Conjugation Biotin
Immunogen Hamster IgG whole molecule

Hamster IgG (H+L chain), adsorbed goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation FITC
Immunogen Hamster IgG whole molecule.

Hamster IgG (H+L chain), adsorbed goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/15,000-1/75,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Conjugation HRP
Immunogen Golden Syrian Hamster IgG, whole molecule in Goat

Hamster IgG (H+L chain), adsorbed goat polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Hamster IgG whole molecule.

Hamster IgG (H+L chain), adsorbed goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Hamster IgG whole molecule.

Human IgG (H+L chain), adsorbed goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product.
Conjugation AP
Immunogen Human IgG whole molecule.

Human IgG (H+L chain), adsorbed goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Note: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6- sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/80,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Human IgG, whole molecule.

Human IgG (H+L chain), adsorbed goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Other applications not tested.
Conjugation FITC
Immunogen Human IgG, whole molecule.

Human IgG (H+L chain), adsorbed goat polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Human IgG whole molecule.

Human IgG (H+L chain), adsorbed goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Human IgG whole molecule.

Human IgM (Mu chain specific) goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting (1:2,000 - 1:10,000), ELISA (1:20,000 - 1:100,000), Immunohistochemistry (1:1,000 - 1,5,000), Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Human IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:3,000 to 1:17,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Human IgM µ chain.

Human IgM (Mu chain specific) goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation FITC
Immunogen Human IgM m mu chain.

Human IgM (Mu chain specific) goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
ELISA: 1/10,000-1/45,000.
Western blot: 1/1,000-1/5,000.
Immunogen Human IgM mu chains.

Human IgM (Mu chain specific) goat polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Human IgM mu heavy chain.

Human IgM (Mu chain specific) goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Human IgM heavy chain

Human IgA + IgG + IgM goat polyclonal antibody, AP

Applications Suitable for immunoblotting (western blot: 1/500-1/3,000, dot blot), ELISA (1/2,000-1/10,000) and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Conjugation AP
Immunogen Human IgG, IgA and IgM whole molecule.

Human IgA + IgG + IgM goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation FITC
Immunogen Human IgG, IgA and IgM whole molecules

Human IgA + IgG + IgM goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
ELISA : 1:70,000
Western Blot : 1:2,000 - 1:10,000
Immunochemistry: 1:1,000 - 1:5,000
Immunogen Human IgG, IgA and IgM whole molecules

Human IgA + IgG + IgM goat polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Human IgG, IgA and IgM whole molecules