Bovine IgG (H+L chain) goat polyclonal antibody, Biotin
Applications | Suitable for Immunoblotting (1:2,000-1:10,000), ELISA (see below), Immunohistochemistry (1:1000-1:5000), Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 µg of Bovine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:11,000 to 1:45,000 of the reconstitution concentration is suggested for this product. |
Reactivities | Bovine |
Conjugation | Biotin |
Immunogen | Bovine IgG whole molecule. |