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SMURF2 (Myc-DDK-tagged)-Human SMAD specific E3 ubiquitin protein ligase 2 (SMURF2)

Vector pCMV6-Entry
Tag Myc-DDK
Mammalian Cell Selection Neomycin

SMURF2 (GFP-tagged) - Human SMAD specific E3 ubiquitin protein ligase 2 (SMURF2)

Vector pCMV6-AC-GFP
Tag TurboGFP
Mammalian Cell Selection Neomycin
  • TrueORF®

SMURF2 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.

Format 2 gRNA vectors, 1 linear donor
Donor DNA EF1a-GFP-P2A-Puro
KN410866 is the updated version of KN210866.

Lenti ORF clone of Human SMAD specific E3 ubiquitin protein ligase 2 (SMURF2), Myc-DDK-tagged

Vector pLenti-C-Myc-DDK-P2A-Puro
Tag Myc-DDK
Mammalian Cell Selection Puromycin
  • LentiORF®

Lenti ORF particles, SMURF2 (Myc-DDK tagged) - Human SMAD specific E3 ubiquitin protein ligase 2 (SMURF2), 200ul, >10^7 TU/mL

Vector pLenti-C-Myc-DDK-P2A-Puro
Tag Myc-DDK
Mammalian Cell Selection Puromycin
  • LentiORF®

SMURF2 - human gene knockout kit via CRISPR, HDR mediated

Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA GFP-puro

Transient overexpression lysate of SMAD specific E3 ubiquitin protein ligase 2 (SMURF2)

Tag C-Myc/DDK
Expression Host HEK293T
Applications WB

Lenti ORF clone of Human SMAD specific E3 ubiquitin protein ligase 2 (SMURF2), Myc-DDK-tagged

Vector pLenti-C-Myc-DDK
Tag Myc-DDK
Mammalian Cell Selection None
  • LentiORF®

SMURF2 (untagged)-Human SMAD specific E3 ubiquitin protein ligase 2 (SMURF2)

Vector pCMV6-XL6
Tag Tag Free
Mammalian Cell Selection None

SMURF2 HEK293T cell transient overexpression lysate (as WB positive control)

Tag C-Myc/DDK
Expression Host HEK293T
Applications WB

SMURF2 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector

Format Retroviral plasmids
Vector pGFP-V-RS

Lenti ORF clone of Human SMAD specific E3 ubiquitin protein ligase 2 (SMURF2), mGFP tagged

Vector pLenti-C-mGFP
Tag mGFP
Mammalian Cell Selection None
  • LentiORF®

3`UTR clone of SMAD specific E3 ubiquitin protein ligase 2 (SMURF2) for miRNA target validation

Vector pMirTarget
Mammalian Cell Selection Neomycin
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase

SMURF2 - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Format Lentiviral particles
Vector pGFP-C-shLenti

SMURF2 CRISPRa kit - CRISPR gene activation of human SMAD specific E3 ubiquitin protein ligase 2

Format 3gRNAs, 1 scramble ctrl and 1 enhancer vector

qPCR primer pairs and template standards against Homo sapiens gene SMURF2

Application Plasmid of exact quantity for transcript copy number calculation

qSTAR qPCR primer pairs against Homo sapiens gene SMURF2

SMURF2 - human gene knockout kit via CRISPR, HDR mediated

Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA mBFP-Neo

SMURF2 - human gene knockout kit via CRISPR, HDR mediated

Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA Luciferase-Puro

SMURF2 - human gene knockout kit via CRISPR, HDR mediated

Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA RFP-BSD

SMURF2 MS Standard C13 and N15-labeled recombinant protein (NP_073576)

Tag C-Myc/DDK
Expression Host HEK293

Lenti ORF clone of Human SMAD specific E3 ubiquitin protein ligase 2 (SMURF2), mGFP tagged

Vector pLenti-C-mGFP-P2A-Puro
Tag mGFP
Mammalian Cell Selection Puromycin
  • LentiORF®

Transient overexpression of SMURF2 (NM_022739) in HEK293T cells paraffin embedded controls for ICC/IHC staining

SMURF2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector

Format Retroviral plasmids
Vector pRS

Transient overexpression of SMURF2 (NM_022739) in HEK293T cells paraffin embedded 4 um sections, controls for ICC/IHC staining, 5 slides per pack

Transient overexpression of SMURF2 (NM_022739) in HEK293T cells paraffin embedded 4 um sections, controls for ICC/IHC staining, 25 slides per pack