ROCK2 Rabbit Polyclonal Antibody

CAT#: TA357884

ROCK2 Antibody - middle region

Product Images

ROCK2 antibody - middle region (TA357884) validated by WB using OVCAR-3 cell lysate at 1.0ug/ml.

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  WB Suggested Anti-ROCK2 Antibody Positive Control: Lane 1: 80ug mouse brain extractLane 2: 80ug rat brain extract Primary Antibody Dilution : 1:500 Secondary Antibody : IRDye 800 CW goat anti-rabbit from Li-COR Bioscience Secondry Antibody Dilution : 1:20,000 Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science

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  Rabbit Anti-ROCK2 Antibody Catalog Number: TA357884 Formalin Fixed Paraffin Embedded Tissue: Human Urinary Bladder Tissue Observed Staining: Plasma membrane, Nucleus Primary Antibody Concentration: 1:600 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec

Specifications

Product Data

• Applications: IHC, WB
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: Expected reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Zebrafish
  Homology: Cow: 100%; Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 79%; Rat: 100%; Zebrafish: 79%
• Formulation: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Predicted Protein Size: 153kDa
• Gene Name: Rho associated coiled-coil containing protein kinase 2
• Database Link:
  NP_004841
  Entrez Gene 19878 MouseEntrez Gene 9475 Human
  UniProt ID O75116
• Background: The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.
• Synonyms: KIAA0619

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, TGF-beta signaling pathway, Vascular smooth muscle contraction, Wnt signaling pathway