PKC iota (PRKCI) (NM_002740) Human Tagged ORF Clone

CAT#: RC205379

PRKCI (Myc-DDK-tagged)-Human protein kinase C, iota (PRKCI)

Product Images

Western blot validation of overexpression lysate (Cat# LY400965) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205379 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PRKCI protein (Cat# TP305379). The protein was produced from HEK293T cells transfected with PRKCI cDNA clone (Cat# RC205379) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PRKCI
• Synonyms: DXS1179E; nPKC-iota; PKCI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205379 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCACACGGTCGCAGGCGGCGGCAGCGGGGACCATTCCCACCAGGTCCGGGTGAAAGCCTACTACC
  GCGGGGATATCATGATAACACATTTTGAACCTTCCATCTCCTTTGAGGGCCTTTGCAATGAGGTTCGAGA
  CATGTGTTCTTTTGACAACGAACAGCTCTTCACCATGAAATGGATAGATGAGGAAGGAGACCCGTGTACA
  GTATCATCTCAGTTGGAGTTAGAAGAAGCCTTTAGACTTTATGAGCTAAACAAGGATTCTGAACTCTTGA
  TTCATGTGTTCCCTTGTGTACCAGAACGTCCTGGGATGCCTTGTCCAGGAGAAGATAAATCCATCTACCG
  TAGAGGTGCACGCCGCTGGAGAAAGCTTTATTGTGCCAATGGCCACACTTTCCAAGCCAAGCGTTTCAAC
  AGGCGTGCTCACTGTGCCATCTGCACAGACCGAATATGGGGACTTGGACGCCAAGGATATAAGTGCATCA
  ACTGCAAACTCTTGGTTCATAAGAAGTGCCATAAACTCGTCACAATTGAATGTGGGCGGCATTCTTTGCC
  ACAGGAACCAGTGATGCCCATGGATCAGTCATCCATGCATTCTGACCATGCACAGACAGTAATTCCATAT
  AATCCTTCAAGTCATGAGAGTTTGGATCAAGTTGGTGAAGAAAAAGAGGCAATGAACACCAGGGAAAGTG
  GCAAAGCTTCATCCAGTCTAGGTCTTCAGGATTTTGATTTGCTCCGGGTAATAGGAAGAGGAAGTTATGC
  CAAAGTACTGTTGGTTCGATTAAAAAAAACAGATCGTATTTATGCAATGAAAGTTGTGAAAAAAGAGCTT
  GTTAATGATGATGAGGATATTGATTGGGTACAGACAGAGAAGCATGTGTTTGAGCAGGCATCCAATCATC
  CTTTCCTTGTTGGGCTGCATTCTTGCTTTCAGACAGAAAGCAGATTGTTCTTTGTTATAGAGTATGTAAA
  TGGAGGAGACCTAATGTTTCATATGCAGCGACAAAGAAAACTTCCTGAAGAACATGCCAGATTTTACTCT
  GCAGAAATCAGTCTAGCATTAAATTATCTTCATGAGCGAGGGATAATTTATAGAGATTTGAAACTGGACA
  ATGTATTACTGGACTCTGAAGGCCACATTAAACTCACTGACTACGGCATGTGTAAGGAAGGATTACGGCC
  AGGAGATACAACCAGCACTTTCTGTGGTACTCCTAATTACATTGCTCCTGAAATTTTAAGAGGAGAAGAT
  TATGGTTTCAGTGTTGACTGGTGGGCTCTTGGAGTGCTCATGTTTGAGATGATGGCAGGAAGGTCTCCAT
  TTGATATTGTTGGGAGCTCCGATAACCCTGACCAGAACACAGAGGATTATCTCTTCCAAGTTATTTTGGA
  AAAACAAATTCGCATACCACGTTCTATGTCTGTAAAAGCTGCAAGTGTTCTGAAGAGTTTTCTTAATAAG
  GACCCTAAGGAACGATTGGGTTGTCTTCCTCAAACAGGATTTGCTGATATTCAGGGACACCCGTTCTTCC
  GAAATGTTGATTGGGATATGATGGAGCAAAAACAGGTGGTACCTCCCTTTAAACCAAATATTTCTGGGGA
  ATTTGGTTTGGACAACTTTGATTCTCAGTTTACTAATGAACGTGTCCAGCTCACTCCAGATGACGATGAC
  ATTGTGAGGAAGATTGATCAGTCTGAATTTGAAGGTTTTGAGTATATCAATCCTCTTTTGATGTCTGCAG
  AAGAATGTGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205379 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSHTVAGGGSGDHSHQVRVKAYYRGDIMITHFEPSISFEGLCNEVRDMCSFDNEQLFTMKWIDEEGDPCT
  VSSQLELEEAFRLYELNKDSELLIHVFPCVPERPGMPCPGEDKSIYRRGARRWRKLYCANGHTFQAKRFN
  RRAHCAICTDRIWGLGRQGYKCINCKLLVHKKCHKLVTIECGRHSLPQEPVMPMDQSSMHSDHAQTVIPY
  NPSSHESLDQVGEEKEAMNTRESGKASSSLGLQDFDLLRVIGRGSYAKVLLVRLKKTDRIYAMKVVKKEL
  VNDDEDIDWVQTEKHVFEQASNHPFLVGLHSCFQTESRLFFVIEYVNGGDLMFHMQRQRKLPEEHARFYS
  AEISLALNYLHERGIIYRDLKLDNVLLDSEGHIKLTDYGMCKEGLRPGDTTSTFCGTPNYIAPEILRGED
  YGFSVDWWALGVLMFEMMAGRSPFDIVGSSDNPDQNTEDYLFQVILEKQIRIPRSMSVKAASVLKSFLNK
  DPKERLGCLPQTGFADIQGHPFFRNVDWDMMEQKQVVPPFKPNISGEFGLDNFDSQFTNERVQLTPDDDD
  IVRKIDQSEFEGFEYINPLLMSAEECV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002740
• ORF Size: 1761 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002740.1, NM_002740.2, NM_002740.3, NM_002740.4, NP_002731.3
• RefSeq Size: 4884 bp
• RefSeq ORF: 1791 bp
• Locus ID: 5584
• Cytogenetics: 3q26.2
• Domains: PB1, pkinase, S_TK_X, TyrKc, DAG_PE-bind, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Endocytosis, Insulin signaling pathway, Tight junction
• MW: 67.3 kDa
• Gene Summary: 'This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X. [provided by RefSeq, Jul 2008]'