PKC iota (PRKCI) (NM_002740) Human Untagged Clone

CAT#: SC323672

PRKCI (untagged)-Kinase deficient mutant (K283M) of Human protein kinase C, iota (PRKCI)


  "NM_002740" in other vectors (8)

Reconstitution Protocol

USD 760.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PRKCI
Synonyms DXS1179E; nPKC-iota; PKCI
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC323672 sequence for NM_002740 edited (data generated by NextGen Sequencing)
ATGCCGACCCAGAGGGACAGCAGCACCATGTCCCACACGGTCGCAGGCGGCGGCAGCGGG
GACCATTCCCACCAGGTCCGGGTGAAAGCCTACTACCGCGGGGATATCATGATAACACAT
TTTGAACCTTCCATCTCCTTTGAGGGCCTTTGCAATGAGGTTCGAGACATGTGTTCTTTT
GACAACGAACAGCTCTTCACCATGAAATGGATAGATGAGGAAGGAGACCCGTGTACAGTA
TCATCTCAGTTGGAGTTAGAAGAAGCCTTTAGACTTTATGAGCTAAACAAGGATTCTGAA
CTCTTGATTCATGTGTTCCCTTGTGTACCAGAACGTCCTGGGATGCCTTGTCCAGGAGAA
GATAAATCCATCTACCGTAGAGGTGCACGCCGCTGGAGAAAGCTTTATTGTGCCAATGGC
CACACTTTCCAAGCCAAGCGTTTCAACAGGCGTGCTCACTGTGCCATCTGCACAGACCGA
ATATGGGGACTTGGACGCCAAGGATATAAGTGCATCAACTGCAAACTCTTGGTTCATAAG
AAGTGCCATAAACTCGTCACAATTGAATGTGGGCGGCATTCTTTGCCACAGGAACCAGTG
ATGCCCATGGATCAGTCATCCATGCATTCTGACCATGCACAGACAGTAATTCCATATAAT
CCTTCAAGTCATGAGAGTTTGGATCAAGTTGGTGAAGAAAAAGAGGCAATGAACACCAGG
GAAAGTGGCAAAGCTTCATCCAGTCTAGGTCTTCAGGATTTTGATTTGCTCCGGGTAATA
GGAAGAGGAAGTTATGCCAAAGTACTGTTGGTTCGATTAAAAAAAACAGATCGTATTTAT
GCAATGATRGTTGTGAAAAAAGAGCTTGTTAATGATGATGAGGATATTGATTGGGTACAG
ACAGAGAAGCATGTGTTTGAGCAGGCATCCAATCATCCTTTCCTTGTTGGGCTGCATTCT
TGCTTTCAGACAGAAAGCAGATTGTTCTTTGTTATAGAGTATGTAAATGGAGGAGACCTA
ATGTTTCATATGCAGCGACAAAGAAAACTTCCTGAAGAACATGCCAGATTTTACTCTGCA
GAAATCAGTCTAGCATTAAATTATCTTCATGAGCGAGGGATAATTTATAGAGATTTGAAA
CTGGACAATGTATTACTGGACTCTGAAGGCCACATTAAACTCACTGACTACGGCATGTGT
AAGGAAGGATTACGGCCAGGAGATACAACCAGCACTTTCTGTGGTACTCCTAATTACATT
GCTCCTGAAATTTTAAGAGGAGAAGATTATGGTTTCAGTGTTGACTGGTGGGCTCTTGGA
GTGCTCATGTTTGAGATGATGGCAGGAAGGTCTCCATTTGATATTGTTGGGAGCTCCGAT
AACCCTGACCAGAACACAGAGGATTATCTCTTCCAAGTTATTTTGGAAAAACAAATTCGC
ATACCACGTTCTCTGTCTGTAAAAGCTGCAAGTGTTCTGAAGAGTTTTCTTAATAAGGAC
CCTAAGGAACGATTGGGTTGTCATCCTCAAACAGGATTTGCTGATATTCAGGGACACCCG
TTCTTCCGAAATGTTGATTGGGATATGATGGAGCAAAAACAGGTGGTACCTCCCTTTAAA
CCAAATATTTCTGGGGAATTTGGTTTGGACAACTTTGATTCTCAGTTTACTAATGAACCT
GTCCAGCTCACTCCAGATGACGATGACATTGTGAGGAAGATTGATCAGTCTGAATTTGAA
GGTTTTGAGTATATCAATCCTCTTTTGATGTCTGCAGAAGAATGTGTCTGA

Clone variation with respect to NM_002740.5
848 a=>t;849 a=>r
>OriGene 5' read for mutant NM_002740 unedited
ACGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC
CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGGCTGTAGAGGCG
GCGGCGCCTACGGGCAGTGGGAGGAGCCGCGCGGTTCCGGCTGCTCCGGCGAGGCGACCCTTGGGTCGGC
GCTGCGGGCGAGGTGGGCAGGTAGGTGGGCGGACGGCCGCGGTTCTCCGGCAAGCGCAGGCGGCGGAGTC
CCCCACGGCGCCCGAAGCGCCCCCCCCGCACCCCCGGGCCTCCAGCGTTGAGGCGGGGGGAGTGGAGGGA
GGATGGCCGACCCAGAAGGGACAAGCCAGCCACCCATGTTCCCACAACGGTCGCAGGCGCGGCGGGCCGG
CGGGGGAACCATTTCCCACCCAGTTTCCGGGGTGAAAGCCCTATAACCGGGGGGGAAAATAATGATAAAC
CCATTTGGAAACCTCCCCTCCCCCTTTAGGGGGCTTTTGAAATAGGGGTCCAAAAAATTGTGTCCTTTTT
GAACCCAAAAACAGCCTTTTCCCCTGTAAATGGGAATAAGTGAAGAGAGGGAACCCCTGGGTCCAAGTTC
CCTCCATTTGAGATTTAAGAAAACCCTTTAAAACTTTTAAAACCTCAACGGGGTCTCGACACCTGGTACT
ACGGGTCCCCTGTGGGCACCAAACAGCTTCTGGAGACCCTCTCCCCAGAAAAAAAATACCCCCCTCCCCC
TAGGTGCGCCCCCCCCGTGAAAACATCTTTATTGGCGCAGGGGGCACTTTTTAGCACACCATTTTTTCAC
GCGTCGCGCCGTGTGCTCTTCTCGACCACATTGTGGCGTCTCTGGCCCGAGTATAAGTCCTCCGGCACCT
GTGTGTGAACAACCACCACTACACACACATATAGTGTGAGGCGACAGTGTCGCAGAGCACGCTAGGTCTT
GCGATGTATGTCCTCCGATCTCGTACTACGTGACAGCAGGGAGGTATACCTG
>SC323672 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
ATGGATTGCTCGGGTATAGGAGAGGAGTTATGCCAAAGTACTGTTGGTTCGATTAAAAAAAACAGATCGT
ATTTATGCAATGATGGTTGTGAAAAAAGAGCTTGTTAATGATGATGAGGATATTGATTGGGTACAGACAG
AGAAGCATGTGTTTGAGCAGGCATCCAATCATCCTTTCCTTGTTGGGCTGCATTCTTGCTTTCAGACAGA
AAGCAGATTGTTCTTTGTTATAGAGTATGTAAATGGAGGAGACCT
Restriction Sites Please inquire     
ACCN NM_002740
Insert Size 2210 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002740.4, NP_002731.3
RefSeq Size 4878 bp
RefSeq ORF 1764 bp
Locus ID 5584
Cytogenetics 3q26.2
Domains PB1, pkinase, S_TK_X, TyrKc, DAG_PE-bind, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Endocytosis, Insulin signaling pathway, Tight junction
Gene Summary 'This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X. [provided by RefSeq, Jul 2008]'

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