PKC iota (PRKCI) (NM_002740) Human Untagged Clone

CAT#: SC323672

PRKCI (untagged)-Kinase deficient mutant (K283M) of Human protein kinase C, iota (PRKCI)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRKCI
• Synonyms: DXS1179E; nPKC-iota; PKCI
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323672 sequence for NM_002740 edited (data generated by NextGen Sequencing)
  ATGCCGACCCAGAGGGACAGCAGCACCATGTCCCACACGGTCGCAGGCGGCGGCAGCGGG
  GACCATTCCCACCAGGTCCGGGTGAAAGCCTACTACCGCGGGGATATCATGATAACACAT
  TTTGAACCTTCCATCTCCTTTGAGGGCCTTTGCAATGAGGTTCGAGACATGTGTTCTTTT
  GACAACGAACAGCTCTTCACCATGAAATGGATAGATGAGGAAGGAGACCCGTGTACAGTA
  TCATCTCAGTTGGAGTTAGAAGAAGCCTTTAGACTTTATGAGCTAAACAAGGATTCTGAA
  CTCTTGATTCATGTGTTCCCTTGTGTACCAGAACGTCCTGGGATGCCTTGTCCAGGAGAA
  GATAAATCCATCTACCGTAGAGGTGCACGCCGCTGGAGAAAGCTTTATTGTGCCAATGGC
  CACACTTTCCAAGCCAAGCGTTTCAACAGGCGTGCTCACTGTGCCATCTGCACAGACCGA
  ATATGGGGACTTGGACGCCAAGGATATAAGTGCATCAACTGCAAACTCTTGGTTCATAAG
  AAGTGCCATAAACTCGTCACAATTGAATGTGGGCGGCATTCTTTGCCACAGGAACCAGTG
  ATGCCCATGGATCAGTCATCCATGCATTCTGACCATGCACAGACAGTAATTCCATATAAT
  CCTTCAAGTCATGAGAGTTTGGATCAAGTTGGTGAAGAAAAAGAGGCAATGAACACCAGG
  GAAAGTGGCAAAGCTTCATCCAGTCTAGGTCTTCAGGATTTTGATTTGCTCCGGGTAATA
  GGAAGAGGAAGTTATGCCAAAGTACTGTTGGTTCGATTAAAAAAAACAGATCGTATTTAT
  GCAATGATRGTTGTGAAAAAAGAGCTTGTTAATGATGATGAGGATATTGATTGGGTACAG
  ACAGAGAAGCATGTGTTTGAGCAGGCATCCAATCATCCTTTCCTTGTTGGGCTGCATTCT
  TGCTTTCAGACAGAAAGCAGATTGTTCTTTGTTATAGAGTATGTAAATGGAGGAGACCTA
  ATGTTTCATATGCAGCGACAAAGAAAACTTCCTGAAGAACATGCCAGATTTTACTCTGCA
  GAAATCAGTCTAGCATTAAATTATCTTCATGAGCGAGGGATAATTTATAGAGATTTGAAA
  CTGGACAATGTATTACTGGACTCTGAAGGCCACATTAAACTCACTGACTACGGCATGTGT
  AAGGAAGGATTACGGCCAGGAGATACAACCAGCACTTTCTGTGGTACTCCTAATTACATT
  GCTCCTGAAATTTTAAGAGGAGAAGATTATGGTTTCAGTGTTGACTGGTGGGCTCTTGGA
  GTGCTCATGTTTGAGATGATGGCAGGAAGGTCTCCATTTGATATTGTTGGGAGCTCCGAT
  AACCCTGACCAGAACACAGAGGATTATCTCTTCCAAGTTATTTTGGAAAAACAAATTCGC
  ATACCACGTTCTCTGTCTGTAAAAGCTGCAAGTGTTCTGAAGAGTTTTCTTAATAAGGAC
  CCTAAGGAACGATTGGGTTGTCATCCTCAAACAGGATTTGCTGATATTCAGGGACACCCG
  TTCTTCCGAAATGTTGATTGGGATATGATGGAGCAAAAACAGGTGGTACCTCCCTTTAAA
  CCAAATATTTCTGGGGAATTTGGTTTGGACAACTTTGATTCTCAGTTTACTAATGAACCT
  GTCCAGCTCACTCCAGATGACGATGACATTGTGAGGAAGATTGATCAGTCTGAATTTGAA
  GGTTTTGAGTATATCAATCCTCTTTTGATGTCTGCAGAAGAATGTGTCTGA
  
  Clone variation with respect to NM_002740.5
  848 a=>t;849 a=>r
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_002740 unedited
  ACGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC
  CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGGCTGTAGAGGCG
  GCGGCGCCTACGGGCAGTGGGAGGAGCCGCGCGGTTCCGGCTGCTCCGGCGAGGCGACCCTTGGGTCGGC
  GCTGCGGGCGAGGTGGGCAGGTAGGTGGGCGGACGGCCGCGGTTCTCCGGCAAGCGCAGGCGGCGGAGTC
  CCCCACGGCGCCCGAAGCGCCCCCCCCGCACCCCCGGGCCTCCAGCGTTGAGGCGGGGGGAGTGGAGGGA
  GGATGGCCGACCCAGAAGGGACAAGCCAGCCACCCATGTTCCCACAACGGTCGCAGGCGCGGCGGGCCGG
  CGGGGGAACCATTTCCCACCCAGTTTCCGGGGTGAAAGCCCTATAACCGGGGGGGAAAATAATGATAAAC
  CCATTTGGAAACCTCCCCTCCCCCTTTAGGGGGCTTTTGAAATAGGGGTCCAAAAAATTGTGTCCTTTTT
  GAACCCAAAAACAGCCTTTTCCCCTGTAAATGGGAATAAGTGAAGAGAGGGAACCCCTGGGTCCAAGTTC
  CCTCCATTTGAGATTTAAGAAAACCCTTTAAAACTTTTAAAACCTCAACGGGGTCTCGACACCTGGTACT
  ACGGGTCCCCTGTGGGCACCAAACAGCTTCTGGAGACCCTCTCCCCAGAAAAAAAATACCCCCCTCCCCC
  TAGGTGCGCCCCCCCCGTGAAAACATCTTTATTGGCGCAGGGGGCACTTTTTAGCACACCATTTTTTCAC
  GCGTCGCGCCGTGTGCTCTTCTCGACCACATTGTGGCGTCTCTGGCCCGAGTATAAGTCCTCCGGCACCT
  GTGTGTGAACAACCACCACTACACACACATATAGTGTGAGGCGACAGTGTCGCAGAGCACGCTAGGTCTT
  GCGATGTATGTCCTCCGATCTCGTACTACGTGACAGCAGGGAGGTATACCTG
  
  Kinase domain sequence

  >SC323672 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  ATGGATTGCTCGGGTATAGGAGAGGAGTTATGCCAAAGTACTGTTGGTTCGATTAAAAAAAACAGATCGT
  ATTTATGCAATGATGGTTGTGAAAAAAGAGCTTGTTAATGATGATGAGGATATTGATTGGGTACAGACAG
  AGAAGCATGTGTTTGAGCAGGCATCCAATCATCCTTTCCTTGTTGGGCTGCATTCTTGCTTTCAGACAGA
  AAGCAGATTGTTCTTTGTTATAGAGTATGTAAATGGAGGAGACCT
  
• Restriction Sites: Please inquire
• ACCN: NM_002740
• Insert Size: 2210 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002740.4, NP_002731.3
• RefSeq Size: 4878 bp
• RefSeq ORF: 1764 bp
• Locus ID: 5584
• Cytogenetics: 3q26.2
• Domains: PB1, pkinase, S_TK_X, TyrKc, DAG_PE-bind, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Endocytosis, Insulin signaling pathway, Tight junction
• Gene Summary: 'This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X. [provided by RefSeq, Jul 2008]'