PKC iota (PRKCI) (NM_002740) Human Untagged Clone

CAT#: SC318027

PRKCI (untagged)-Human protein kinase C, iota (PRKCI)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRKCI
• Synonyms: DXS1179E; nPKC-iota; PKCI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002740, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCGACCCAGAGGGACAGCAGCACCATGTCCCACACGGTCGCAGGCGGCGGCAGCGGGGACCATTCCC
  ACCAGGTCCGGGTGAAAGCCTACTACCGCGGGGATATCATGATAACACATTTTGAACCTTCCATCTCCTT
  TGAGGGCCTTTGCAATGAGGTTCGAGACATGTGTTCTTTTGACAACGAACAGCTCTTCACCATGAAATGG
  ATAGATGAGGAAGGAGACCCGTGTACAGTATCATCTCAGTTGGAGTTAGAAGAAGCCTTTAGACTTTATG
  AGCTAAACAAGGATTCTGAACTCTTGATTCATGTGTTCCCTTGTGTACCAGAACGTCCTGGGATGCCTTG
  TCCAGGAGAAGATAAATCCATCTACCGTAGAGGTGCACGCCGCTGGAGAAAGCTTTATTGTGCCAATGGC
  CACACTTTCCAAGCCAAGCGTTTCAACAGGCGTGCTCACTGTGCCATCTGCACAGACCGAATATGGGGAC
  TTGGACGCCAAGGATATAAGTGCATCAACTGCAAACTCTTGGTTCATAAGAAGTGCCATAAACTCGTCAC
  AATTGAATGTGGGCGGCATTCTTTGCCACAGGAACCAGTGATGCCCATGGATCAGTCATCCATGCATTCT
  GACCATGCACAGACAGTAATTCCATATAATCCTTCAAGTCATGAGAGTTTGGATCAAGTTGGTGAAGAAA
  AAGAGGCAATGAACACCAGGGAAAGTGGCAAAGCTTCATCCAGTCTAGGTCTTCAGGATTTTGATTTGCT
  CCGGGTAATAGGAAGAGGAAGTTATGCCAAAGTACTGTTGGTTCGATTAAAAAAAACAGATCGTATTTAT
  GCAATGAAAGTTGTGAAAAAAGAGCTTGTTAATGATGATGAGGATATTGATTGGGTACAGACAGAGAAGC
  ATGTGTTTGAGCAGGCATCCAATCATCCTTTCCTTGTTGGGCTGCATTCTTGCTTTCAGACAGAAAGCAG
  ATTGTTCTTTGTTATAGAGTATGTAAATGGAGGAGACCTAATGTTTCATATGCAGCGACAAAGAAAACTT
  CCTGAAGAACATGCCAGATTTTACTCTGCAGAAATCAGTCTAGCATTAAATTATCTTCATGAGCGAGGGA
  TAATTTATAGAGATTTGAAACTGGACAATGTATTACTGGACTCTGAAGGCCACATTAAACTCACTGACTA
  CGGCATGTGTAAGGAAGGATTACGGCCAGGAGATACAACCAGCACTTTCTGTGGTACTCCTAATTACATT
  GCTCCTGAAATTTTAAGAGGAGAAGATTATGGTTTCAGTGTTGACTGGTGGGCTCTTGGAGTGCTCATGT
  TTGAGATGATGGCAGGAAGGTCTCCATTTGATATTGTTGGGAGCTCCGATAACCCTGACCAGAACACAGA
  GGATTATCTCTTCCAAGTTATTTTGGAAAAACAAATTCGCATACCACGTTCTCTGTCTGTAAAAGCTGCA
  AGTGTTCTGAAGAGTTTTCTTAATAAGGACCCTAAGGAACGATTGGGTTGTCATCCTCAAACAGGATTTG
  CTGATATTCAGGGACACCCGTTCTTCCGAAATGTTGATTGGGATATGATGGAGCAAAAACAGGTGGTACC
  TCCCTTTAAACCAAATATTTCTGGGGAATTTGGTTTGGACAACTTTGATTCTCAGTTTACTAATGAACCT
  GTCCAGCTCACTCCAGATGACGATGACATTGTGAGGAAGATTGATCAGTCTGAATTTGAAGGTTTTGAGT
  ATATCAATCCTCTTTTGATGTCTGCAGAAGAATGTGTCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_002740
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002740.5, NP_002731.4
• RefSeq Size: 4884 bp
• RefSeq ORF: 1791 bp
• Locus ID: 5584
• Cytogenetics: 3q26.2
• Domains: PB1, pkinase, S_TK_X, TyrKc, DAG_PE-bind, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Endocytosis, Insulin signaling pathway, Tight junction
• Gene Summary: 'This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X. [provided by RefSeq, Jul 2008]'