PKC iota (PRKCI) (NM_002740) Human Untagged Clone

CAT#: SC118455

PRKCI (untagged)-Human protein kinase C, iota (PRKCI)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRKCI
• Synonyms: DXS1179E; nPKC-iota; PKCI
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002740 edited
  GCGGCTGTAGAGGCGGCGGCGCCTACGGGCAGTGGGAGGAGCCGCGCGGTTCCGGCTGCT
  CCGGCGAGGCGACCCTTGGGTCGGCGCTGCGGGCGAGGTGGGCAGGTAGGTGGGCGGACG
  GCCGCGGTTCTCCGGCAAGCGCAGGCGGCGGAGTCCCCCACGGCGCCCGAAGCGCCCCCC
  CGCACCCCCGGCCTCCAGCGTTGAGGCGGGGGAGTGAGGAGATGCCGACCCAGAGGGACA
  GCAGCACCATGTCCCACACGGTCGCAGGCGGCGGCAGCGGGGACCATTCCCACCAGGTCC
  GGGTGAAAGCCTACTACCGCGGGGATATCATGATAACACATTTTGAACCTTCCATCTCCT
  TTGAGGGCCTTTGCAATGAGGTTCGAGACATGTGTTCTTTTGACAACGAACAGCTCTTCA
  CCATGAAATGGATAGATGAGGAAGGAGACCCGTGTACAGTATCATCTCAGTTGGAGTTAG
  AAGAAGCCTTTAGACTTTATGAGCTAAACAAGGATTCTGAACTCTTGATTCATGTGTTCC
  CTTGTGTACCAGAACGTCCTGGGATGCCTTGTCCAGGAGAAGATAAATCCATCTACCGTA
  GAGGTGCACGCCGCTGGAGAAAGCTTTATTGTGCCAATGGCCACACTTTCCAAGCCAAGC
  GTTTCAACAGGCGTGCTCACTGTGCCATCTGCACAGACCGAATATGGGGACTTGGACGCC
  AAGGATATAAGTGCATCAACTGCAAACTCTTGGTTCATAAGAAGTGCCATAAACTCGTCA
  CAATTGAATGTGGGCGGCATTCTTTGCCACAGGAACCAGTGATGCCCATGGATCAGTCAT
  CCATGCATTCTGACCATGCACAGACAGTAATTCCATATAATCCTTCAAGTCATGAGAGTT
  TGGATCAAGTTGGTGAAGAAAAAGAGGCAATGAACACCAGGGAAAGTGGCAAAGCTTCAT
  CCAGTCTAGGTCTTCAGGATTTTGATTTGCTCCGGGTAATAGGAAGAGGAAGTTATGCCA
  AAGTACTGTTGGTTCGATTAAAAAAAACAGATCGTATTTATGCAATGAAAGTTGTGAAAA
  AAGAGCTTGTTAATGATGATGAGGATATTGATTGGGTACAGACAGAGAAGCATGTGTTTG
  AGCAGGCATCCAATCATCCTTTCCTTGTTGGGCTGCATTCTTGCTTTCAGACAGAAAGCA
  GATTGTTCTTTGTTATAGAGTATGTAAATGGAGGAGACCTAATGTTTCATATGCAGCGAC
  AAAGAAAACTTCCTGAAGAACATGCCAGATTTTACTCTGCAGAAATCAGTCTAGCATTAA
  ATTATCTTCATGAGCGAGGGATAATTTATAGAGATTTGAAACTGGACAATGTATTACTGG
  ACTCTGAAGGCCACATTAAACTCACTGACTACGGCATGTGTAAGGAAGGATTACGGCCAG
  GAGATACAACCAGCACTTTCTGTGGTACTCCTAATTACATTGCTCCTGAAATTTTAAGAG
  GAGAAGATTATGGTTTCAGTGTTGACTGGTGGGCTCTTGGAGTGCTCATGTTTGAGATGA
  TGGCAGGAAGGTCTCCATTTGATATTGTTGGGAGCTCCGATAACCCTGACCAGAACACAG
  AGGATTATCTCTTCCAAGTTATTTTGGAAAAACAAATTCGCATACCACGTTCTCTGTCTG
  TAAAAGCTGCAAGTGTTCTGAAGAGTTTTCTTAATAAGGACCCTAAGGAACGATTGGGTT
  GTCATCCTCAAACAGGATTTGCTGATATTCAGGGACACCCGTTCTTCCGAAATGTTGATT
  GGGATATGATGGAGCAAAAACAGGTGGTACCTCCCTTTAAACCAAATATTTCTGGGGAAT
  TTGGTTTGGACAACTTTGATTCTCAGTTTACTAATGAACCTGTCCAGCTCACTCCAGATG
  ACGATGACATTGTGAGGAAGATTGATCAGTCTGAATTTGAAGGTTTTGAGTATATCAATC
  CTCTTTTGATGTCTGCAGAAGAATGTGTCTGATCCTCATTTTTCAACCATGTATTCTACT
  CATGTTGCCATTTAATGCATGGATAAACTTGCTGCAAGCCTGGATACAATTAACCATTTT
  ATATTTGCCACCTACAAAAAAACACCCAATATCTTCTCTTGTAGACTATATGAATCAATT
  ATTACATCTGTTTTACTATGAAAAAAAAATTAATACTACTAGCTTCCAGACAATCATGTC
  AAAATTTAGTTGAACTGGTTTTTCAGTTTTTAAAAGGCCTACAGATGAGTAATGAAGTTA
  TCTTTTTTGTTTAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002740 unedited
  NGTCAAAATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGCGGCTGTA
  GAGGCGGCGGCGCCTACGGGCAGTGGGAGGAGCCGCGCGGTTCCGGCTGCTCCGGCGAGG
  CGACCCTTGGGTCGGCGCTGCGGGCGAGGTGGGCAGGTAGGTGGGCGGACGGCCGCGGTT
  CTCCGGCAAGCGCAGGCGGCGGAGTCCCCCACGGCGCCCGAAGCGCCCCCCCGCACCCCC
  GGCCTCCAGCGTTGAGGCGGGGGAGTGAGGAGATGCCGACCCAGAGGGACAGCAGCACCA
  TGTCCCACACGGTCGCAGGCGGCGGCAGCGGGGACCATTCCCACCAGGTCCGGGTGAAAG
  CCTACTACCGCGGGGATATCATGATAACACATTTTGAACCTTCCATCTCCTTTGAGGGCC
  TTTGCAATGAGGTTCGAGACATGTGTTCTTTTGACAACGAACAGCTCTTCACCATGAAAT
  GGATAGATGAGGAAGGAGACCCGTGTACAGTATCATCTCAGTTGGAGTTAGAAGAAGCCT
  TTAGACTTTATGAGCTAAACAAGGATTCTGAACTCTTGATTCATGTGTTCCCTTGTGTAC
  CAGAACGTCCTGGGATGCCTTGTCCAGGAGAAGATAAATCCATCTACCGTAGAGGTGCAC
  GCCGCTGGAGAAAGCTNTATTGTGCCAATGGCCACACTTTCCAAGCCAAGCGTTTCAACA
  GGCGTGCTCACTGTGCCATCTGCACAGACCGAATATGGGGACTTGGACGCCAGGATATAA
  GTGCATCAACTGCAAACTCTTGGTTCATAAAGAGTGCCATANACTCGTCACAATTGATGT
  GGGCGGCATTCTTTGCCACAGAACCAGTGATGCCATGGATCAGTCTNCATGCATTCTGAC
  ATGCCAGACC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002740 unedited
  CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAACAAAAAAGAAACTTC
  ATTACTCATCTGTAGGCCTTTTAAAAACTGAAAAACCAGTTCAACTAAATTTTGACATGA
  TTGTCTGGAAGCTAGTAGTATTAATTTTTTTTTCATAGTAAAACAGATGTAATAATTGAT
  TCATATAGTCTACAAGAGAAGATATTGGGTGTTTTTTTGTAGGTGGCAAATATAAAATGG
  TTAATTGTATCCAGGCTTGCAGCAAGTTTATCCATGCATTAAATGGCAACATGAGTAGAA
  TACATGGTTGAAAAATGAGGATCAGACACATTCTTCTGCAGACATCAAAAGAGGATTGAT
  ATACTCAAAACCTTCAAATTCAGACTGATCAATCTTCCTCACAATGTCATCGTCATCTGG
  AGTGAGCTGGACAGGTTCATTAGTAAACTGAGAATCAAAGTTGTCCAAACCAAATTCCCC
  AGAAATATTTGGTTTAAAGGGAGGTACCACCTGTTTTTGCTCCATCATATCCCAATCAAC
  ATTTCGGAAGAACGGGTGTCCCTGAATATCAGCAAATCCTGTTTGAGGATGACAACCCAA
  TCGTTCCTTAGGGTCCTTATTAAGAAAACTCTTCAGAACACTTGCAGCTTTTACAGACAG
  AGAACGTGGTATGCGAATTTGTTTTTCCAAAATAACTTGGAAGAGATAATCCTCTGTGTT
  CTGGTCAGGGTTATNCGAGCTCCCAACATATAAATGGAGACCTTNCTGCATCATCTCAAC
  ATGAGCACTNCAGAGCCCACAGTCACACTGAAACCCATATCTCTCTCTTAAATTCAGAGC
  ATGTATTTAGAGTCACAGAAGTGCTGGNTGATCTCTGGCGTAATCTCCTACCATGCGTAT
  CAGGAGTTATGTGGCTCANATCAGATACATGTCAGTTAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002740
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002740.4, NP_002731.3
• RefSeq Size: 4878 bp
• RefSeq ORF: 1764 bp
• Locus ID: 5584
• Cytogenetics: 3q26.2
• Domains: PB1, pkinase, S_TK_X, TyrKc, DAG_PE-bind, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Endocytosis, Insulin signaling pathway, Tight junction
• Gene Summary: 'This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X. [provided by RefSeq, Jul 2008]'