S (NC_002645) Virus Tagged ORF Clone

CAT#: VC100563

Myc-DDK-tagged ORF clone of viral ORF for surface glycoprotein [Human coronavirus 229E], codon optimized for human cell expression, NP_073551

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: S
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100563 represents NCBI reference of NP_073551 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCGTTCTCCTGGTGGCCTATGCCCTGTTGCACATTGCAGGATGCCAGACAACCAACGGACTGAACA
  CCAGTTACTCTGTGTGTAACGGCTGTGTGGGTTACTCCGAGAACGTGTTCGCAGTGGAAAGTGGCGGTTA
  TATACCTTCTGACTTTGCATTTAATAACTGGTTTCTGCTCACCAATACTTCAAGCGTGGTAGATGGGGTC
  GTAAGGTCCTTCCAACCATTGCTCCTTAACTGTTTGTGGAGCGTGAGCGGGCTTAGATTCACCACAGGCT
  TTGTATACTTCAATGGGACTGGCCGGGGTGATTGCAAAGGGTTCTCAAGCGATGTGCTGTCCGATGTAAT
  TAGGTATAATCTCAATTTCGAAGAAAATCTGCGAAGAGGAACAATACTCTTTAAGACTAGCTACGGGGTG
  GTTGTCTTCTACTGCACCAATAACACTCTCGTTTCAGGGGATGCGCATATTCCATTTGGCACAGTGCTTG
  GGAATTTTTATTGCTTCGTGAATACAACGATCGGGAACGAGACCACAAGTGCCTTCGTTGGTGCACTTCC
  AAAGACGGTGCGAGAGTTTGTTATCTCCCGAACTGGGCACTTTTACATTAACGGTTACAGGTACTTTACC
  CTGGGAAACGTCGAGGCCGTAAATTTTAACGTCACGACTGCTGAGACAACCGACTTTTGTACCGTGGCCC
  TGGCTTCCTACGCCGATGTATTGGTGAACGTGAGCCAGACCTCTATCGCTAACATCATCTACTGCAACTC
  CGTAATCAACAGACTCCGGTGTGACCAGTTGTCTTTCGACGTCCCTGACGGCTTCTACTCCACGAGTCCT
  ATTCAGTCAGTCGAATTGCCGGTGAGTATTGTTAGCCTGCCCGTCTACCACAAACACACATTCATTGTGC
  TGTACGTAGATTTTAAACCTCAGTCTGGCGGAGGAAAGTGCTTTAATTGCTACCCTGCAGGTGTCAATAT
  TACCCTTGCTAACTTCAATGAAACCAAAGGTCCTTTGTGTGTCGACACATCCCATTTCACTACAAAGTAT
  GTGGCGGTCTATGCCAACGTGGGGAGGTGGTCAGCCTCAATAAATACTGGCAATTGCCCTTTTAGTTTTG
  GAAAGGTCAACAATTTCGTCAAATTTGGGAGCGTCTGCTTTAGCCTCAAAGACATTCCCGGTGGTTGCGC
  CATGCCGATTGTTGCTAATTGGGCCTATAGCAAATACTATACAATAGGAAGCTTGTACGTCAGCTGGAGT
  GATGGCGATGGGATTACGGGTGTCCCGCAGCCCGTGGAGGGGGTGTCTTCATTTATGAACGTGACCCTTG
  ACAAGTGCACCAAGTACAATATCTACGATGTGTCCGGGGTTGGCGTTATAAGAGTCTCCAACGATACATT
  CCTCAACGGTATCACCTACACCAGTACGTCTGGAAATCTTCTCGGCTTTAAGGACGTGACTAAGGGCACA
  ATATACTCTATCACCCCATGCAATCCCCCCGACCAACTTGTTGTCTACCAGCAGGCCGTGGTAGGCGCTA
  TGCTCTCTGAAAACTTTACGTCATACGGGTTCTCCAATGTCGTAGAACTGCCAAAATTCTTTTATGCCTC
  TAACGGGACTTACAACTGTACCGACGCAGTGTTGACCTACAGCAGCTTCGGTGTGTGTGCCGACGGTTCC
  ATCATTGCCGTGCAGCCTCGAAATGTGAGCTACGATAGCGTTAGCGCGATCGTAACGGCCAACTTGAGCA
  TCCCTTCAAACTGGACCACTTCAGTTCAGGTGGAATATTTGCAGATTACCTCTACACCTATTGTAGTTGA
  TTGCTCCACATATGTGTGTAACGGAAATGTGCGGTGCGTGGAACTCCTCAAGCAATATACAAGCGCCTGT
  AAGACAATCGAGGACGCCCTGCGGAATAGCGCAAGGCTGGAGTCTGCCGATGTCAGTGAAATGCTTACCT
  TCGATAAGAAGGCTTTCACGTTGGCAAATGTATCCTCATTTGGCGATTATAATCTCTCCAGTGTCATTCC
  TAGTCTCCCCACAAGTGGGAGTAGAGTGGCCGGACGAAGCGCCATTGAAGACATCCTCTTCTCCAAGCTC
  GTCACAAGTGGTCTGGGCACTGTGGACGCAGATTATAAAAAGTGTACTAAAGGCCTTAGCATCGCTGATC
  TCGCATGTGCACAGTACTACAACGGGATTATGGTGCTGCCTGGCGTGGCCGATGCTGAACGCATGGCTAT
  GTATACCGGATCCCTCATAGGTGGCATAGCTCTTGGGGGACTTACCAGTGCGGTGAGCATACCATTCTCT
  CTCGCTATCCAGGCACGGCTGAACTATGTTGCATTGCAGACAGATGTGCTGCAGGAAAACCAGAAGATCC
  TGGCCGCATCTTTCAACAAGGCAATGACAAACATCGTGGACGCCTTCACTGGCGTTAATGACGCCATCAC
  ACAGACAAGCCAAGCACTCCAGACCGTGGCCACCGCCCTGAACAAGATTCAGGATGTGGTGAACCAGCAA
  GGCAACTCCCTGAATCACCTCACCAGTCAGTTGCGCCAAAATTTTCAGGCGATCTCTTCATCCATTCAGG
  CAATATACGACCGCCTGGATACCATACAGGCGGATCAGCAAGTTGACCGCCTGATAACCGGACGACTCGC
  CGCCCTGAATGTGTTTGTTAGTCATACTCTGACAAAATATACCGAAGTTCGAGCTTCCAGGCAACTCGCA
  CAGCAGAAAGTGAACGAGTGCGTCAAGAGTCAGAGCAAGAGGTACGGCTTTTGTGGTAATGGCACCCACA
  TCTTCTCCATCGTGAACGCTGCACCTGAGGGATTGGTGTTTCTGCATACAGTACTTCTGCCCACTCAATA
  TAAAGATGTTGAGGCCTGGTCTGGGCTTTGCGTAGATGGAACCAACGGGTACGTGCTGAGACAACCCAAC
  CTCGCTCTCTATAAGGAAGGCAATTATTATCGGATAACCTCCCGGATTATGTTCGAGCCTCGCATCCCAA
  CTATGGCCGACTTCGTGCAGATTGAGAATTGTAATGTCACGTTCGTGAATATATCTCGGTCCGAACTGCA
  GACTATCGTTCCAGAATATATTGACGTAAATAAAACGCTCCAGGAGCTGAGTTACAAGTTGCCAAACTAC
  ACAGTCCCTGATCTGGTCGTGGAACAATACAATCAGACTATCCTGAACCTGACTAGCGAGATTAGCACCC
  TGGAGAATAAGTCCGCTGAACTGAACTATACGGTTCAGAAACTGCAGACGCTGATTGACAACATAAACAG
  CACCCTGGTGGACCTGAAGTGGCTGAACCGCGTTGAAACTTACATCAAGTGGCCGTGGTGGGTCTGGCTT
  TGTATTTCTGTCGTGCTGATTTTCGTGGTCTCAATGTTGCTGCTGTGTTGTTGCTCAACTGGTTGCTGTG
  GATTTTTTTCATGTTTCGCTTCCAGCATCAGAGGATGTTGCGAGAGTACCAAGCTGCCCTATTACGATGT
  GGAAAAAATCCACATACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100563 representing NP_073551
  Red=Cloning sites Green=Tags
  
  MFVLLVAYALLHIAGCQTTNGLNTSYSVCNGCVGYSENVFAVESGGYIPSDFAFNNWFLLTNTSSVVDGV
  VRSFQPLLLNCLWSVSGLRFTTGFVYFNGTGRGDCKGFSSDVLSDVIRYNLNFEENLRRGTILFKTSYGV
  VVFYCTNNTLVSGDAHIPFGTVLGNFYCFVNTTIGNETTSAFVGALPKTVREFVISRTGHFYINGYRYFT
  LGNVEAVNFNVTTAETTDFCTVALASYADVLVNVSQTSIANIIYCNSVINRLRCDQLSFDVPDGFYSTSP
  IQSVELPVSIVSLPVYHKHTFIVLYVDFKPQSGGGKCFNCYPAGVNITLANFNETKGPLCVDTSHFTTKY
  VAVYANVGRWSASINTGNCPFSFGKVNNFVKFGSVCFSLKDIPGGCAMPIVANWAYSKYYTIGSLYVSWS
  DGDGITGVPQPVEGVSSFMNVTLDKCTKYNIYDVSGVGVIRVSNDTFLNGITYTSTSGNLLGFKDVTKGT
  IYSITPCNPPDQLVVYQQAVVGAMLSENFTSYGFSNVVELPKFFYASNGTYNCTDAVLTYSSFGVCADGS
  IIAVQPRNVSYDSVSAIVTANLSIPSNWTTSVQVEYLQITSTPIVVDCSTYVCNGNVRCVELLKQYTSAC
  KTIEDALRNSARLESADVSEMLTFDKKAFTLANVSSFGDYNLSSVIPSLPTSGSRVAGRSAIEDILFSKL
  VTSGLGTVDADYKKCTKGLSIADLACAQYYNGIMVLPGVADAERMAMYTGSLIGGIALGGLTSAVSIPFS
  LAIQARLNYVALQTDVLQENQKILAASFNKAMTNIVDAFTGVNDAITQTSQALQTVATALNKIQDVVNQQ
  GNSLNHLTSQLRQNFQAISSSIQAIYDRLDTIQADQQVDRLITGRLAALNVFVSHTLTKYTEVRASRQLA
  QQKVNECVKSQSKRYGFCGNGTHIFSIVNAAPEGLVFLHTVLLPTQYKDVEAWSGLCVDGTNGYVLRQPN
  LALYKEGNYYRITSRIMFEPRIPTMADFVQIENCNVTFVNISRSELQTIVPEYIDVNKTLQELSYKLPNY
  TVPDLVVEQYNQTILNLTSEISTLENKSAELNYTVQKLQTLIDNINSTLVDLKWLNRVETYIKWPWWVWL
  CISVVLIFVVSMLLLCCCSTGCCGFFSCFASSIRGCCESTKLPYYDVEKIHIQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_002645
• ORF Size: 3519 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_002645.1, NP_073551
• RefSeq ORF: 3519
• Locus ID: 918758
• MW: 128.6 kDa